Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 29-10-2014

Detalles Bibliográficos
Autor: García Galán, Cristina
Tipo de recurso: tesis doctoral
Fecha de publicación:2014
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:español
OAI Identifier:oai:repositorio.uam.es:10486/663592
Acceso en línea:http://hdl.handle.net/10486/663592
Access Level:acceso abierto
Palabra clave:Enzimas - Tesis doctorales
Lipasa - Tesis doctorales
Biología y Biomedicina / Biología
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spelling Diseño de biocatalizadores de lipasas y su aplicación en bioprocesosGarcía Galán, CristinaEnzimas - Tesis doctoralesLipasa - Tesis doctoralesBiología y Biomedicina / BiologíaTesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 29-10-2014The need for updating the existing processes, or finding new methods to meet environmental and legislation requirements, is an imminent task for all Chemical Industries. The path towards sustainability encourages the interest to find alternatives to traditional methods; this way, biocatalysis appears as a potential alternative to cope with the basis of a ‘greener’ chemistry. The use of enzymes as catalysts includes important advantages: mild operational conditions (temperature, pressure and pH), the possibility of shortening the steps of synthesis using high efficient, and selective transformations, as well as, obtaining safer and purer chiral products. Nevertheless, to call the attention of industrial transformations, enzymes must gather a set of improvements before their application. In this concern, in the last decades, a wide research in different enzimatical, biological and engineering fields, has been developed and definitely, contributed to the emergence of the first biotransformations in the Industry. Immobilization is a powerful tool that when carefully designed, could deal with some of the drawbacks of enzymes as biocatalists for industrial applications, and improve interesting properties; this tool is the simplest way for to reduce the cost of the enzymatic biocatalists, making them reusable; but it also may stabilize the enzyme or change its specificity. In this thesis, a group of lipases were immobilized in different hydrophobic supports. This is an easy-­‐to-­‐perform method that gathers immobilization and purification. The pool of immobilized lipases prepared, and other commercial available biocatalists, were studied and compared in terms of stability against several inactivating media and activity using different substrates. Two important biotransformations were also studied using these biocatalysts; esterifications, employed for the production of aroma and flavours under natural label, and transesterification, for the production of biodiesel. Albeit, results obtained showed some negative effects regarding thermal stability, thanks to this simple way for the immobilization of lipases, it could be possible to partition hydrophilic and deleterious substances away from the hydrophobic microenvironment of the immobilized enzyme. Thus, the immobilized lipases could be used in very heterogeneous media were several phases exist and even mechanical agitation is not capable to promote mass transfer through the different phases. Finally, the use of agitation under ultrasound technology was analyzed as an approach to improve mass transfer in esterification; results showed improvements especially in the reactions carried out by commercial biocatalysts immobilized in hydrophilic supportsFernández Lafuente, RobertoCosta Rodrigues, RafaelDepartamento de Biología MolecularFacultad de Ciencias20142014-10-29doctoral thesishttp://purl.org/coar/resource_type/c_db06NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10486/663592reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridEspañolspaopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6635922026-06-23T12:46:27Z
dc.title.none.fl_str_mv Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
title Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
spellingShingle Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
García Galán, Cristina
Enzimas - Tesis doctorales
Lipasa - Tesis doctorales
Biología y Biomedicina / Biología
title_short Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
title_full Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
title_fullStr Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
title_full_unstemmed Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
title_sort Diseño de biocatalizadores de lipasas y su aplicación en bioprocesos
dc.creator.none.fl_str_mv García Galán, Cristina
author García Galán, Cristina
author_facet García Galán, Cristina
author_role author
dc.contributor.none.fl_str_mv Fernández Lafuente, Roberto
Costa Rodrigues, Rafael
Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Enzimas - Tesis doctorales
Lipasa - Tesis doctorales
Biología y Biomedicina / Biología
topic Enzimas - Tesis doctorales
Lipasa - Tesis doctorales
Biología y Biomedicina / Biología
description Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 29-10-2014
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-10-29
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/663592
url http://hdl.handle.net/10486/663592
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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