Extensive translatome remodeling during ER stress response in mammalian cells

In this work we have described the translatome of two mammalian cell lines, NIH3T3 and Jurkat, by scoring the relative polysome association of ~10,000 mRNA under normal and ER stress conditions. We have found that translation efficiencies of mRNA correlated poorly with transcript abundance, although...

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Autores: Ventoso, Iván, Kochetov, Alex, Montaner, David, Dopazo, Joaquín, Santoyo, Javier
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/113105
Acceso en línea:http://hdl.handle.net/10261/113105
Access Level:acceso abierto
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spelling Extensive translatome remodeling during ER stress response in mammalian cellsVentoso, IvánKochetov, AlexMontaner, DavidDopazo, JoaquínSantoyo, JavierIn this work we have described the translatome of two mammalian cell lines, NIH3T3 and Jurkat, by scoring the relative polysome association of ~10,000 mRNA under normal and ER stress conditions. We have found that translation efficiencies of mRNA correlated poorly with transcript abundance, although a general tendency was observed so that the highest translation efficiencies were found in abundant mRNA. Despite the differences found between mouse (NIH3T3) and human (Jurkat) cells, both cell types share a common translatome composed by ~800-900 mRNA that encode proteins involved in basic cellular functions. Upon stress, an extensive remodeling in translatomes was observed so that translation of ~50% of mRNA was inhibited in both cell types, this effect being more dramatic for those mRNA that accounted for most of the cell translation. Interestingly, we found two subsets comprising 1000-1500 mRNA whose translation resisted or was induced by stress. Translation arrest resistant class includes many mRNA encoding aminoacyl tRNA synthetases, ATPases and enzymes involved in DNA replication and stress response such as BiP. This class of mRNA is characterized by high translation rates in both control and stress conditions. Translation inducible class includes mRNA whose translation was relieved after stress, showing a high enrichment in early response transcription factors of bZIP and zinc finger C2H2 classes. Unlike yeast, a general coordination between changes in translation and transcription upon stress (potentiation) was not observed in mammalian cells. Among the different features of mRNA analyzed, we found a relevant association of translation efficiency with the presence of upstream ATG in the 5′UTR and with the length of coding sequence of mRNA, and a looser association with other parameters such as the length and the G+C content of 5′UTR. A model for translatome remodeling during the acute phase of stress response in mammalian cells is proposed. © 2012 Ventoso et al.Fundación Mutua Madrileña; Ministerio de Ciencia e Innovación; Generalitat Valenciana-Fondo Europeo Desarrollo Regional (GVA-FEDER); The Centro de Investigaciones Biomédicas en Red (CIBER) de Enfermedades Raras is an initiative of the Instituto de Salud carlos III (ISCIII); Red Temática de Investigación Cooperativa en Cáncer (RTICC); Instituto de Salud Carlos III (ISCIII); Ministerio de Ciencia e Innovación; Programme of Russian Academy of Sciences, Russian Ministry of Science & Education; Fundación Ramón Areces Peer ReviewedPublic Library of ScienceFundación Mutua MadrileñaMinisterio de Ciencia e Innovación (España)Generalitat ValencianaCentro de Investigación Biomédica en Red Enfermedades Raras (España)Red Temática de Investigación Cooperativa en Cáncer (España)Instituto de Salud Carlos IIIRussian Academy of SciencesMinistry of Education and Science of the Russian FederationFundación Ramón Areces2015201520122015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/113105reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1131052026-05-22T06:33:51Z
dc.title.none.fl_str_mv Extensive translatome remodeling during ER stress response in mammalian cells
title Extensive translatome remodeling during ER stress response in mammalian cells
spellingShingle Extensive translatome remodeling during ER stress response in mammalian cells
Ventoso, Iván
title_short Extensive translatome remodeling during ER stress response in mammalian cells
title_full Extensive translatome remodeling during ER stress response in mammalian cells
title_fullStr Extensive translatome remodeling during ER stress response in mammalian cells
title_full_unstemmed Extensive translatome remodeling during ER stress response in mammalian cells
title_sort Extensive translatome remodeling during ER stress response in mammalian cells
dc.creator.none.fl_str_mv Ventoso, Iván
Kochetov, Alex
Montaner, David
Dopazo, Joaquín
Santoyo, Javier
author Ventoso, Iván
author_facet Ventoso, Iván
Kochetov, Alex
Montaner, David
Dopazo, Joaquín
Santoyo, Javier
author_role author
author2 Kochetov, Alex
Montaner, David
Dopazo, Joaquín
Santoyo, Javier
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fundación Mutua Madrileña
Ministerio de Ciencia e Innovación (España)
Generalitat Valenciana
Centro de Investigación Biomédica en Red Enfermedades Raras (España)
Red Temática de Investigación Cooperativa en Cáncer (España)
Instituto de Salud Carlos III
Russian Academy of Sciences
Ministry of Education and Science of the Russian Federation
Fundación Ramón Areces
description In this work we have described the translatome of two mammalian cell lines, NIH3T3 and Jurkat, by scoring the relative polysome association of ~10,000 mRNA under normal and ER stress conditions. We have found that translation efficiencies of mRNA correlated poorly with transcript abundance, although a general tendency was observed so that the highest translation efficiencies were found in abundant mRNA. Despite the differences found between mouse (NIH3T3) and human (Jurkat) cells, both cell types share a common translatome composed by ~800-900 mRNA that encode proteins involved in basic cellular functions. Upon stress, an extensive remodeling in translatomes was observed so that translation of ~50% of mRNA was inhibited in both cell types, this effect being more dramatic for those mRNA that accounted for most of the cell translation. Interestingly, we found two subsets comprising 1000-1500 mRNA whose translation resisted or was induced by stress. Translation arrest resistant class includes many mRNA encoding aminoacyl tRNA synthetases, ATPases and enzymes involved in DNA replication and stress response such as BiP. This class of mRNA is characterized by high translation rates in both control and stress conditions. Translation inducible class includes mRNA whose translation was relieved after stress, showing a high enrichment in early response transcription factors of bZIP and zinc finger C2H2 classes. Unlike yeast, a general coordination between changes in translation and transcription upon stress (potentiation) was not observed in mammalian cells. Among the different features of mRNA analyzed, we found a relevant association of translation efficiency with the presence of upstream ATG in the 5′UTR and with the length of coding sequence of mRNA, and a looser association with other parameters such as the length and the G+C content of 5′UTR. A model for translatome remodeling during the acute phase of stress response in mammalian cells is proposed. © 2012 Ventoso et al.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/113105
url http://hdl.handle.net/10261/113105
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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