Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma

Activation of ras oncogenes is found in human cancers; overall it is observed in 15% of all neoplasms. The purpose of this study was to assess the extent of involvement of ras oncogenes in osteosarcoma. Tumor samples from a series of 49 pediatric patients diagnosed with osteosarcoma and treated at o...

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Autores: Antillon, F. (Federico)|||/items/6ca2623f-bfbb-4801-b529-06c1b4d1b212, García-Delgado, M. (Marina)|||/items/9ccbb6ed-82f7-42ef-9394-f23bef9580c0, Villa-Elizaga, I. (Ignacio)|||/items/0cce72cc-cc2a-4ffa-b85a-f2eb7c3d2f10, Sierrasesumaga, L. (Luis)|||/items/64d1f030-95e5-4055-a33a-920166ac53a3
Tipo de recurso: artículo
Fecha de publicación:1995
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/23761
Acceso en línea:https://hdl.handle.net/10171/23761
Access Level:acceso abierto
Palabra clave:Bone Neoplasms/genetics
Osteosarcoma/genetics
DNA, Neoplasm/analysis
Unidad de Genética clínica
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spelling Mutational Activation of ras Genes is Absent in Pediatric OsteosarcomaAntillon, F. (Federico)|||/items/6ca2623f-bfbb-4801-b529-06c1b4d1b212García-Delgado, M. (Marina)|||/items/9ccbb6ed-82f7-42ef-9394-f23bef9580c0Villa-Elizaga, I. (Ignacio)|||/items/0cce72cc-cc2a-4ffa-b85a-f2eb7c3d2f10Sierrasesumaga, L. (Luis)|||/items/64d1f030-95e5-4055-a33a-920166ac53a3Bone Neoplasms/geneticsOsteosarcoma/geneticsDNA, Neoplasm/analysisUnidad de Genética clínicaActivation of ras oncogenes is found in human cancers; overall it is observed in 15% of all neoplasms. The purpose of this study was to assess the extent of involvement of ras oncogenes in osteosarcoma. Tumor samples from a series of 49 pediatric patients diagnosed with osteosarcoma and treated at our institution were evaluated. Paraffin-embedded tumor samples from diagnostic biopsies, from tumor en bloc resection tissue after neoadjuvant chemotherapy, and samples from metastases were examined in search of point mutations in H, K, and N-ras genes at codons 12 and 61 by means of polymerase chain reaction (PCR), slot-blotting, and radioactive labeled specific DNA probes. A total of 92 archival samples were studied. No point mutations activating these genes were found. These findings suggest that the activation by point mutations at codons 12 and 61 of the H, K, and N-ras genes does not play a role in the pathogenesis of human osteosarcoma. Since no point mutations in codons 12 and 61 were detected, it was not possible to establish any correlation between the ras genes and clinical or histologic findingsElsevierDadun. Depósito Académico Digital Universidad de Navarra20122012-11-2219951995-01-0119951995-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/23761reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/237612026-06-21T12:47:57Z
dc.title.none.fl_str_mv Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
title Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
spellingShingle Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
Antillon, F. (Federico)|||/items/6ca2623f-bfbb-4801-b529-06c1b4d1b212
Bone Neoplasms/genetics
Osteosarcoma/genetics
DNA, Neoplasm/analysis
Unidad de Genética clínica
title_short Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
title_full Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
title_fullStr Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
title_full_unstemmed Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
title_sort Mutational Activation of ras Genes is Absent in Pediatric Osteosarcoma
dc.creator.none.fl_str_mv Antillon, F. (Federico)|||/items/6ca2623f-bfbb-4801-b529-06c1b4d1b212
García-Delgado, M. (Marina)|||/items/9ccbb6ed-82f7-42ef-9394-f23bef9580c0
Villa-Elizaga, I. (Ignacio)|||/items/0cce72cc-cc2a-4ffa-b85a-f2eb7c3d2f10
Sierrasesumaga, L. (Luis)|||/items/64d1f030-95e5-4055-a33a-920166ac53a3
author Antillon, F. (Federico)|||/items/6ca2623f-bfbb-4801-b529-06c1b4d1b212
author_facet Antillon, F. (Federico)|||/items/6ca2623f-bfbb-4801-b529-06c1b4d1b212
García-Delgado, M. (Marina)|||/items/9ccbb6ed-82f7-42ef-9394-f23bef9580c0
Villa-Elizaga, I. (Ignacio)|||/items/0cce72cc-cc2a-4ffa-b85a-f2eb7c3d2f10
Sierrasesumaga, L. (Luis)|||/items/64d1f030-95e5-4055-a33a-920166ac53a3
author_role author
author2 García-Delgado, M. (Marina)|||/items/9ccbb6ed-82f7-42ef-9394-f23bef9580c0
Villa-Elizaga, I. (Ignacio)|||/items/0cce72cc-cc2a-4ffa-b85a-f2eb7c3d2f10
Sierrasesumaga, L. (Luis)|||/items/64d1f030-95e5-4055-a33a-920166ac53a3
author2_role author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Bone Neoplasms/genetics
Osteosarcoma/genetics
DNA, Neoplasm/analysis
Unidad de Genética clínica
topic Bone Neoplasms/genetics
Osteosarcoma/genetics
DNA, Neoplasm/analysis
Unidad de Genética clínica
description Activation of ras oncogenes is found in human cancers; overall it is observed in 15% of all neoplasms. The purpose of this study was to assess the extent of involvement of ras oncogenes in osteosarcoma. Tumor samples from a series of 49 pediatric patients diagnosed with osteosarcoma and treated at our institution were evaluated. Paraffin-embedded tumor samples from diagnostic biopsies, from tumor en bloc resection tissue after neoadjuvant chemotherapy, and samples from metastases were examined in search of point mutations in H, K, and N-ras genes at codons 12 and 61 by means of polymerase chain reaction (PCR), slot-blotting, and radioactive labeled specific DNA probes. A total of 92 archival samples were studied. No point mutations activating these genes were found. These findings suggest that the activation by point mutations at codons 12 and 61 of the H, K, and N-ras genes does not play a role in the pathogenesis of human osteosarcoma. Since no point mutations in codons 12 and 61 were detected, it was not possible to establish any correlation between the ras genes and clinical or histologic findings
publishDate 1995
dc.date.none.fl_str_mv 1995
1995-01-01
1995
1995-01-01
2012
2012-11-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/23761
url https://hdl.handle.net/10171/23761
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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