Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus
A replication-defective recombinant adenovirus (RAd), RAdCMV-CE1, containing core and E1 genes of hepatitis C virus (HCV) was constructed. RAdCMV-CE1 was able to express core and E1 proteins both in mice and human cells. Immunization of BALB/c mice with RAdCMV-CE1 induced a specific cytotoxic T-cell...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 1997 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/21662 |
| Acceso en línea: | https://hdl.handle.net/10171/21662 |
| Access Level: | acceso abierto |
| Palabra clave: | Defective Viruses/immunology Hepacivirus/immunology T-Lymphocytes, Cytotoxic/immunology Viral Core Proteins/immunology Viral Envelope Proteins/immunology |
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Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirusBruña-Romero, O. (Oscar)|||/items/a10bef04-a282-4ea4-a58e-7b570690c04fLasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0cWilkinson, G. (Gavin)|||/items/ff263d1b-b59c-4dfd-9696-ad75c56803edGrace, K. (Ken)|||/items/c37e648b-dc8c-44b0-a2dd-54e40106f0ecKlarke, B. (Berwyn)|||/items/ca3574c1-0a97-4cd4-91d0-f0752dc4931bBorras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8ccPrieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71Defective Viruses/immunologyHepacivirus/immunologyT-Lymphocytes, Cytotoxic/immunologyViral Core Proteins/immunologyViral Envelope Proteins/immunologyA replication-defective recombinant adenovirus (RAd), RAdCMV-CE1, containing core and E1 genes of hepatitis C virus (HCV) was constructed. RAdCMV-CE1 was able to express core and E1 proteins both in mice and human cells. Immunization of BALB/c mice with RAdCMV-CE1 induced a specific cytotoxic T-cell response against the two HCV proteins. This response was characterized using a panel of 60 synthetic 14- or 15-mer overlapping peptides (10 amino-acid overlap) spanning the entire sequence of these proteins. Five main epitopes were found in the core protein, four of which had been previously described either in mice or humans. One single novel epitope was found in E1. Fine mapping of this E1 determinant, showed that octamer GHRMAWDM is the minimal epitope recognized by cytotoxic T lymphocytes (CTL). The cytotoxic T-cell response was H-2d restricted, lasted for at least 100 days, and was mediated by T cells with the classic CD4-CD8+ phenotype. This work demonstrates that replication-defective recombinant adenoviruses can efficiently express HCV proteins and are able to induce an in vivo cytotoxic T-cell response against a diversity of epitopes from HCV antigens. These vectors should be taken into consideration in the design of vaccines and also as a means to stimulate specific T-cell responses in chronic HCV carriers.Wiley-BlackwellDadun. Depósito Académico Digital Universidad de Navarra20122012-04-1719971997-01-0119971997-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/21662reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/216622026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| title |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| spellingShingle |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus Bruña-Romero, O. (Oscar)|||/items/a10bef04-a282-4ea4-a58e-7b570690c04f Defective Viruses/immunology Hepacivirus/immunology T-Lymphocytes, Cytotoxic/immunology Viral Core Proteins/immunology Viral Envelope Proteins/immunology |
| title_short |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| title_full |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| title_fullStr |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| title_full_unstemmed |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| title_sort |
Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus |
| dc.creator.none.fl_str_mv |
Bruña-Romero, O. (Oscar)|||/items/a10bef04-a282-4ea4-a58e-7b570690c04f Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c Wilkinson, G. (Gavin)|||/items/ff263d1b-b59c-4dfd-9696-ad75c56803ed Grace, K. (Ken)|||/items/c37e648b-dc8c-44b0-a2dd-54e40106f0ec Klarke, B. (Berwyn)|||/items/ca3574c1-0a97-4cd4-91d0-f0752dc4931b Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8cc Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 |
| author |
Bruña-Romero, O. (Oscar)|||/items/a10bef04-a282-4ea4-a58e-7b570690c04f |
| author_facet |
Bruña-Romero, O. (Oscar)|||/items/a10bef04-a282-4ea4-a58e-7b570690c04f Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c Wilkinson, G. (Gavin)|||/items/ff263d1b-b59c-4dfd-9696-ad75c56803ed Grace, K. (Ken)|||/items/c37e648b-dc8c-44b0-a2dd-54e40106f0ec Klarke, B. (Berwyn)|||/items/ca3574c1-0a97-4cd4-91d0-f0752dc4931b Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8cc Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 |
| author_role |
author |
| author2 |
Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c Wilkinson, G. (Gavin)|||/items/ff263d1b-b59c-4dfd-9696-ad75c56803ed Grace, K. (Ken)|||/items/c37e648b-dc8c-44b0-a2dd-54e40106f0ec Klarke, B. (Berwyn)|||/items/ca3574c1-0a97-4cd4-91d0-f0752dc4931b Borras-Cuesta, F. (Francisco)|||/items/9f3719bd-4cf6-4e5b-b672-39179b54e8cc Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Defective Viruses/immunology Hepacivirus/immunology T-Lymphocytes, Cytotoxic/immunology Viral Core Proteins/immunology Viral Envelope Proteins/immunology |
| topic |
Defective Viruses/immunology Hepacivirus/immunology T-Lymphocytes, Cytotoxic/immunology Viral Core Proteins/immunology Viral Envelope Proteins/immunology |
| description |
A replication-defective recombinant adenovirus (RAd), RAdCMV-CE1, containing core and E1 genes of hepatitis C virus (HCV) was constructed. RAdCMV-CE1 was able to express core and E1 proteins both in mice and human cells. Immunization of BALB/c mice with RAdCMV-CE1 induced a specific cytotoxic T-cell response against the two HCV proteins. This response was characterized using a panel of 60 synthetic 14- or 15-mer overlapping peptides (10 amino-acid overlap) spanning the entire sequence of these proteins. Five main epitopes were found in the core protein, four of which had been previously described either in mice or humans. One single novel epitope was found in E1. Fine mapping of this E1 determinant, showed that octamer GHRMAWDM is the minimal epitope recognized by cytotoxic T lymphocytes (CTL). The cytotoxic T-cell response was H-2d restricted, lasted for at least 100 days, and was mediated by T cells with the classic CD4-CD8+ phenotype. This work demonstrates that replication-defective recombinant adenoviruses can efficiently express HCV proteins and are able to induce an in vivo cytotoxic T-cell response against a diversity of epitopes from HCV antigens. These vectors should be taken into consideration in the design of vaccines and also as a means to stimulate specific T-cell responses in chronic HCV carriers. |
| publishDate |
1997 |
| dc.date.none.fl_str_mv |
1997 1997-01-01 1997 1997-01-01 2012 2012-04-17 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/21662 |
| url |
https://hdl.handle.net/10171/21662 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
| publisher.none.fl_str_mv |
Wiley-Blackwell |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869418939053768704 |
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15,300724 |