Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma
Despite its relative low incidence, PDAC is one of the most aggressive and lethal types of cancer, being currently the seventh leading cause of cancer death worldwide, with a 5-year survival rate of 10.8%. Taking into consideration the necessity to improve the prognosis of these patients, this resea...
| Autores: | , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de Cantabria (UC) |
| Repositorio: | UCrea Repositorio Abierto de la Universidad de Cantabria |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unican.es:10902/27860 |
| Acceso en línea: | https://hdl.handle.net/10902/27860 |
| Access Level: | acceso abierto |
| Palabra clave: | Pancreatic ductal adenocarcinoma (PDAC) Biomarkers Resectable disease Borderline disease Cytokines and growth factors T lymphocytes B lymphocytes Protein arrays Flow cytometry and immunohistochemistry |
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| dc.title.none.fl_str_mv |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| title |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| spellingShingle |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma González-Borja, Iranzu Pancreatic ductal adenocarcinoma (PDAC) Biomarkers Resectable disease Borderline disease Cytokines and growth factors T lymphocytes B lymphocytes Protein arrays Flow cytometry and immunohistochemistry |
| title_short |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| title_full |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| title_fullStr |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| title_full_unstemmed |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| title_sort |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinoma |
| dc.creator.none.fl_str_mv |
González-Borja, Iranzu Viúdez, Antonio Alors-Pérez, Emilia Goñi, Saioa Amat, Irene Ghanem, Ismael Pazo-Cid, Roberto Feliu, Jaimen Alonso, Laura López López, Carlos|||0000-0002-8901-741X Arrazubi, Virginia Gallego, Javier Pérez-Sanz, Jairo Hernández-García, Irene Vera, Ruth Castaño, Justo P. Fernández-Irigoyen, Joaquín López López, Carlos|||0000-0002-8901-741X |
| author |
González-Borja, Iranzu |
| author_facet |
González-Borja, Iranzu Viúdez, Antonio Alors-Pérez, Emilia Goñi, Saioa Amat, Irene Ghanem, Ismael Pazo-Cid, Roberto Feliu, Jaimen Alonso, Laura López López, Carlos|||0000-0002-8901-741X Arrazubi, Virginia Gallego, Javier Pérez-Sanz, Jairo Hernández-García, Irene Vera, Ruth Castaño, Justo P. Fernández-Irigoyen, Joaquín |
| author_role |
author |
| author2 |
Viúdez, Antonio Alors-Pérez, Emilia Goñi, Saioa Amat, Irene Ghanem, Ismael Pazo-Cid, Roberto Feliu, Jaimen Alonso, Laura López López, Carlos|||0000-0002-8901-741X Arrazubi, Virginia Gallego, Javier Pérez-Sanz, Jairo Hernández-García, Irene Vera, Ruth Castaño, Justo P. Fernández-Irigoyen, Joaquín |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad de Cantabria |
| dc.subject.none.fl_str_mv |
Pancreatic ductal adenocarcinoma (PDAC) Biomarkers Resectable disease Borderline disease Cytokines and growth factors T lymphocytes B lymphocytes Protein arrays Flow cytometry and immunohistochemistry |
| topic |
Pancreatic ductal adenocarcinoma (PDAC) Biomarkers Resectable disease Borderline disease Cytokines and growth factors T lymphocytes B lymphocytes Protein arrays Flow cytometry and immunohistochemistry |
| description |
Despite its relative low incidence, PDAC is one of the most aggressive and lethal types of cancer, being currently the seventh leading cause of cancer death worldwide, with a 5-year survival rate of 10.8%. Taking into consideration the necessity to improve the prognosis of these patients, this research has been focused on the discovery of new biomarkers. For this purpose, patients with BL and resectable disease were recruited. Serum cytokines and growth factors were monitored at different time points using protein arrays. Immune cell populations were determined by flow cytometry in peripheral blood as well as by immunohistochemistry (IHC) in tumor tissues. Several cytokines were found to be differentially expressed between the study subgroups. In the BL disease setting, two different scores were proven to be independent prognostic factors for progression-free survival (PFS) (based on IL-10, MDC, MIF, and eotaxin-3) and OS (based on eotaxin-3, NT-3, FGF-9, and IP10). In the same context, CA19-9 was found to play a role as independent prognostic factor for OS. Eotaxin-3 and MDC cytokines for PFS, and eotaxin-3, NT-3, and CKβ8-1 for OS, were shown to be predictive biomarkers for nab-paclitaxel and gemcitabine regimen. Similarly, oncostatin, BDNF, and IP10 cytokines were proven to act as predictive biomarkers regarding PFS, for FOLFIRINOX regimen. In the resectable cohort, RANTES, TIMP-1, FGF-4, and IL-10 individually differentiated patients according to their cancer-associated survival. Regarding immune cell populations, baseline high levels of circulating B lymphocytes were related to a significantly longer OS, while these levels significantly decreased as progression occurred. Similarly, baseline high levels of helper lymphocytes (CD4+), low levels of cytotoxic lymphocytes (CD8+), and a high CD4/CD8 ratio, were related to a significantly longer PFS. Finally, high levels of CD4+ and CD8+ intratumoural infiltration was associated with significantly longer PFS. In conclusion, in this study we were able to identify several prognostic and predictive biomarker candidates in patients diagnosed of resectable or BL PDAC. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-12-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10902/27860 |
| url |
https://hdl.handle.net/10902/27860 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
Cancers, 2022, 14, 5993 reponame:UCrea Repositorio Abierto de la Universidad de Cantabria instname:Universidad de Cantabria (UC) |
| instname_str |
Universidad de Cantabria (UC) |
| reponame_str |
UCrea Repositorio Abierto de la Universidad de Cantabria |
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UCrea Repositorio Abierto de la Universidad de Cantabria |
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|
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|
| _version_ |
1869418936431280128 |
| spelling |
Cytokines and lymphoid populations as potential biomarkers in locally and borderline pancreatic adenocarcinomaGonzález-Borja, IranzuViúdez, AntonioAlors-Pérez, EmiliaGoñi, SaioaAmat, IreneGhanem, IsmaelPazo-Cid, RobertoFeliu, JaimenAlonso, LauraLópez López, Carlos|||0000-0002-8901-741XArrazubi, VirginiaGallego, JavierPérez-Sanz, JairoHernández-García, IreneVera, RuthCastaño, Justo P.Fernández-Irigoyen, JoaquínLópez López, Carlos|||0000-0002-8901-741XPancreatic ductal adenocarcinoma (PDAC)BiomarkersResectable diseaseBorderline diseaseCytokines and growth factorsT lymphocytesB lymphocytesProtein arraysFlow cytometry and immunohistochemistryDespite its relative low incidence, PDAC is one of the most aggressive and lethal types of cancer, being currently the seventh leading cause of cancer death worldwide, with a 5-year survival rate of 10.8%. Taking into consideration the necessity to improve the prognosis of these patients, this research has been focused on the discovery of new biomarkers. For this purpose, patients with BL and resectable disease were recruited. Serum cytokines and growth factors were monitored at different time points using protein arrays. Immune cell populations were determined by flow cytometry in peripheral blood as well as by immunohistochemistry (IHC) in tumor tissues. Several cytokines were found to be differentially expressed between the study subgroups. In the BL disease setting, two different scores were proven to be independent prognostic factors for progression-free survival (PFS) (based on IL-10, MDC, MIF, and eotaxin-3) and OS (based on eotaxin-3, NT-3, FGF-9, and IP10). In the same context, CA19-9 was found to play a role as independent prognostic factor for OS. Eotaxin-3 and MDC cytokines for PFS, and eotaxin-3, NT-3, and CKβ8-1 for OS, were shown to be predictive biomarkers for nab-paclitaxel and gemcitabine regimen. Similarly, oncostatin, BDNF, and IP10 cytokines were proven to act as predictive biomarkers regarding PFS, for FOLFIRINOX regimen. In the resectable cohort, RANTES, TIMP-1, FGF-4, and IL-10 individually differentiated patients according to their cancer-associated survival. Regarding immune cell populations, baseline high levels of circulating B lymphocytes were related to a significantly longer OS, while these levels significantly decreased as progression occurred. Similarly, baseline high levels of helper lymphocytes (CD4+), low levels of cytotoxic lymphocytes (CD8+), and a high CD4/CD8 ratio, were related to a significantly longer PFS. Finally, high levels of CD4+ and CD8+ intratumoural infiltration was associated with significantly longer PFS. In conclusion, in this study we were able to identify several prognostic and predictive biomarker candidates in patients diagnosed of resectable or BL PDAC.This work was funded by grants from the Department of Health from the Government of Navarra (Ref. 008-2018), REFBIO II Pyrenees Biomedical Network from Programa INTERREG V-A España-Francia-Andorra (Ref. BMK_PANC) and Sociedad Española de Oncología Médica (SEOM) to A.V. I.G.B was supported by a predoctoral fellowship from the Department of Economic Development Government of Navarre Ayudas para la contratación de doctorandos y doctorandas por empresas y organismos de investigación y difusión de conocimientos: doctorados industriales 2018–2020. Intensification Programme Navarrabiomed 2017–2021 Obra Social La Caixa Fundación Caja Navarra. Work by JPC received funds from Spanish Ministry of Economy (MINECO; BFU2016–80360-R) and Ministry of Science and Innovation (MICINN; PID2019-105201RB-I00), Junta de Andalucía (BIO- 0139), Universidad de Córdoba-FEDER (UCO-202099901918904), GETNE2019 Research grant; and CIBERobn Fisiopatología de la Obesidad y Nutrición (CIBER is an initiative of Instituto de Salud Carlos III, co-funded by European Union: ERDF/ESF, “Investing in your future”). EAP was funded by a Predoctoral contract FI17/00282 Instituto de Salud Carlos III.MDPIUniversidad de Cantabria20222022-12-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttps://hdl.handle.net/10902/27860Cancers, 2022, 14, 5993reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/278602026-06-02T12:39:31Z |
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15,300724 |