Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors

Background: Small RNAs (sRNAs), including small interfering RNAs (siRNAs) and microRNAs (miRNAs), have emerged as important regulators of eukaryotic gene expression. In plants, miRNAs play critical roles in development, nutrient homeostasis and abiotic stress responses. Accumulating evidence also re...

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Autores: Baldrich, Patricia|||0000-0003-4669-6632, Kakar, Klementina, Siré, Christelle, Moreno, Ana Beatriz|||0000-0002-1522-8118, Berger, Angélique, Garcia i Chapa, Meritxell, Lopez-Moya, Juan Jose|||0000-0002-3176-9651, Riechmann, José Luis|||0000-0003-1108-1945, San Segundo, Blanca|||0000-0001-7409-3172
Tipo de documento: artigo
Data de publicação:2014
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:179933
Acesso em linha:https://ddd.uab.cat/record/179933
https://dx.doi.org/urn:doi:10.1186/1471-2164-15-1083
Access Level:Acceso aberto
Palavra-chave:Arabidopsis
Microarray analysis
MicroRNA
Fungal elicitors
Hc-siRNA
MiR168 sensor
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spelling Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitorsBaldrich, Patricia|||0000-0003-4669-6632Kakar, KlementinaSiré, ChristelleMoreno, Ana Beatriz|||0000-0002-1522-8118Berger, AngéliqueGarcia i Chapa, MeritxellLopez-Moya, Juan Jose|||0000-0002-3176-9651Riechmann, José Luis|||0000-0003-1108-1945San Segundo, Blanca|||0000-0001-7409-3172ArabidopsisMicroarray analysisMicroRNAFungal elicitorsHc-siRNAMiR168 sensorBackground: Small RNAs (sRNAs), including small interfering RNAs (siRNAs) and microRNAs (miRNAs), have emerged as important regulators of eukaryotic gene expression. In plants, miRNAs play critical roles in development, nutrient homeostasis and abiotic stress responses. Accumulating evidence also reveals that sRNAs are involved in plant immunity. Most studies on pathogen-regulated sRNAs have been conducted in Arabidopsis plants infected with the bacterial pathogen Pseudomonas syringae, or treated with the flagelin-derived elicitor peptide flg22 from P. syringae. This work investigates sRNAs that are regulated by elicitors from the fungus Fusarium oxysporum in Arabidopsis. - Results: Microarray analysis revealed alterations on the accumulation of a set of sRNAs in response to elicitor treatment, including miRNAs and small RNA sequences derived from massively parallel signature sequencing. Among the elicitor-regulated miRNAs was miR168 which regulates ARGONAUTE1, the core component of the RNA-induced silencing complex involved in miRNA functioning. Promoter analysis in transgenic Arabidopsis plants revealed transcriptional activation of MIR168 by fungal elicitors. Furthermore, transgenic plants expressing a GFP-miR168 sensor gene confirmed that the elicitor-induced miR168 is active. MiR823, targeting Chromomethylase3 (CMT3) involved in RNA-directed DNA methylation (RdDM) was also found to be regulated by fungal elicitors. In addition to known miRNAs, microarray analysis allowed the identification of an elicitor-inducible small RNA that was incorrectly annotated as a miRNA. Studies on Arabidopsis mutants impaired in small RNA biogenesis demonstrated that this sRNA, is a heterochromatic-siRNA (hc-siRNA) named as siRNA415. Hc-siRNAs are known to be involved in RNA-directed DNA methylation (RdDM). SiRNA415 is detected in several plant species. - Conclusion: Results here presented support a transcriptional regulatory mechanism underlying MIR168 expression. This finding highlights the importance of miRNA functioning in adaptive processes of Arabidopsis plants to fungal infection. The results of this study also lay a foundation for the involvement of RdDM processes through the activity of siRNA415 and miR823 in mediating regulation of immune responses in Arabidopsis plants. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/179933https://dx.doi.org/urn:doi:10.1186/1471-2164-15-1083reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2012-32838Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BES-2010-032879Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2010-14949Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BIO2009-08719Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BFU2008-04251open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1799332026-06-06T12:50:31Z
dc.title.none.fl_str_mv Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
title Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
spellingShingle Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
Baldrich, Patricia|||0000-0003-4669-6632
Arabidopsis
Microarray analysis
MicroRNA
Fungal elicitors
Hc-siRNA
MiR168 sensor
title_short Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
title_full Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
title_fullStr Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
title_full_unstemmed Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
title_sort Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors
dc.creator.none.fl_str_mv Baldrich, Patricia|||0000-0003-4669-6632
Kakar, Klementina
Siré, Christelle
Moreno, Ana Beatriz|||0000-0002-1522-8118
Berger, Angélique
Garcia i Chapa, Meritxell
Lopez-Moya, Juan Jose|||0000-0002-3176-9651
Riechmann, José Luis|||0000-0003-1108-1945
San Segundo, Blanca|||0000-0001-7409-3172
author Baldrich, Patricia|||0000-0003-4669-6632
author_facet Baldrich, Patricia|||0000-0003-4669-6632
Kakar, Klementina
Siré, Christelle
Moreno, Ana Beatriz|||0000-0002-1522-8118
Berger, Angélique
Garcia i Chapa, Meritxell
Lopez-Moya, Juan Jose|||0000-0002-3176-9651
Riechmann, José Luis|||0000-0003-1108-1945
San Segundo, Blanca|||0000-0001-7409-3172
author_role author
author2 Kakar, Klementina
Siré, Christelle
Moreno, Ana Beatriz|||0000-0002-1522-8118
Berger, Angélique
Garcia i Chapa, Meritxell
Lopez-Moya, Juan Jose|||0000-0002-3176-9651
Riechmann, José Luis|||0000-0003-1108-1945
San Segundo, Blanca|||0000-0001-7409-3172
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
Microarray analysis
MicroRNA
Fungal elicitors
Hc-siRNA
MiR168 sensor
topic Arabidopsis
Microarray analysis
MicroRNA
Fungal elicitors
Hc-siRNA
MiR168 sensor
description Background: Small RNAs (sRNAs), including small interfering RNAs (siRNAs) and microRNAs (miRNAs), have emerged as important regulators of eukaryotic gene expression. In plants, miRNAs play critical roles in development, nutrient homeostasis and abiotic stress responses. Accumulating evidence also reveals that sRNAs are involved in plant immunity. Most studies on pathogen-regulated sRNAs have been conducted in Arabidopsis plants infected with the bacterial pathogen Pseudomonas syringae, or treated with the flagelin-derived elicitor peptide flg22 from P. syringae. This work investigates sRNAs that are regulated by elicitors from the fungus Fusarium oxysporum in Arabidopsis. - Results: Microarray analysis revealed alterations on the accumulation of a set of sRNAs in response to elicitor treatment, including miRNAs and small RNA sequences derived from massively parallel signature sequencing. Among the elicitor-regulated miRNAs was miR168 which regulates ARGONAUTE1, the core component of the RNA-induced silencing complex involved in miRNA functioning. Promoter analysis in transgenic Arabidopsis plants revealed transcriptional activation of MIR168 by fungal elicitors. Furthermore, transgenic plants expressing a GFP-miR168 sensor gene confirmed that the elicitor-induced miR168 is active. MiR823, targeting Chromomethylase3 (CMT3) involved in RNA-directed DNA methylation (RdDM) was also found to be regulated by fungal elicitors. In addition to known miRNAs, microarray analysis allowed the identification of an elicitor-inducible small RNA that was incorrectly annotated as a miRNA. Studies on Arabidopsis mutants impaired in small RNA biogenesis demonstrated that this sRNA, is a heterochromatic-siRNA (hc-siRNA) named as siRNA415. Hc-siRNAs are known to be involved in RNA-directed DNA methylation (RdDM). SiRNA415 is detected in several plant species. - Conclusion: Results here presented support a transcriptional regulatory mechanism underlying MIR168 expression. This finding highlights the importance of miRNA functioning in adaptive processes of Arabidopsis plants to fungal infection. The results of this study also lay a foundation for the involvement of RdDM processes through the activity of siRNA415 and miR823 in mediating regulation of immune responses in Arabidopsis plants.
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/179933
https://dx.doi.org/urn:doi:10.1186/1471-2164-15-1083
url https://ddd.uab.cat/record/179933
https://dx.doi.org/urn:doi:10.1186/1471-2164-15-1083
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2012-32838
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BES-2010-032879
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2010-14949
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BIO2009-08719
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BFU2008-04251
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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