In vitro and in vivo anti-Candida activity of citral in combination with fluconazole

[EN] Background The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. Aim The aim of this study was to evaluate the antifungal activity of citral, a phyt...

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Autores: Miranda Cadena, Katherine, Marcos Arias, Cristina, Pérez Rodríguez, Aitzol, Cabello Beitia, Iván, Mateo Alesanco, Estibaliz, Sevillano Peña, Elena, Madariaga Torres, Lucila, Quindós Andrés, Guillermo, Eraso Barrio, María Elena
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/56734
Acceso en línea:http://hdl.handle.net/10810/56734
Access Level:acceso abierto
Palabra clave:candida
biofilm
citral
azole resistance
caenorhabditis elegans
gene expression
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spelling In vitro and in vivo anti-Candida activity of citral in combination with fluconazoleMiranda Cadena, KatherineMarcos Arias, CristinaPérez Rodríguez, AitzolCabello Beitia, IvánMateo Alesanco, EstibalizSevillano Peña, ElenaMadariaga Torres, LucilaQuindós Andrés, GuillermoEraso Barrio, María Elenacandidabiofilmcitralazole resistancecaenorhabditis elegansgene expression[EN] Background The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. Aim The aim of this study was to evaluate the antifungal activity of citral, a phytocompound present in lemongrass essential oil, in monotherapy and combined with fluconazole against azole-resistant Candida planktonic cells and biofilms. The effect of citral combined with fluconazole was also analysed with regard to the expression of fluconazole resistance-associated genes in Candida albicans and the effectiveness of the combination therapy in a Caenorhabditis elegans model of candidiasis. Results Citral reduced biofilm formation at initial stages and the metabolic activity of the mature biofilm. The combination of citral with fluconazole was synergistic, with a significant increase in the survival of C. elegans infected with Candida. RNA analysis revealed a reduction of the expression of the efflux pump encoded by MDR1, leading to a greater effect of fluconazole. Conclusion Citral in monotherapy and in combination with fluconazole could represent an interesting therapy to treat recalcitrant Candida infections associated to biofilms.This research was supported by Gobierno Vasco-Eusko Jaurlaritza [Eusko Jaurlaritza GIC15/78 IT-990-16] and by Fundacion ONCE "Oportunidad al Talento" and Fondo Social Europeo CM-A [C.M.-A.]; Ministerio de Economia, Industria y Competitividad, Gobierno de Espan [PID2020-117983RB-I00].Taylor & Francis202220222022info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/56734reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/PID2020-117983RB-I00/https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2045813info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/es/© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Atribución-NoComercial 3.0 Españaoai:addi.ehu.eus:10810/567342026-06-18T09:23:17Z
dc.title.none.fl_str_mv In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
spellingShingle In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
Miranda Cadena, Katherine
candida
biofilm
citral
azole resistance
caenorhabditis elegans
gene expression
title_short In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_full In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_fullStr In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_full_unstemmed In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_sort In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
dc.creator.none.fl_str_mv Miranda Cadena, Katherine
Marcos Arias, Cristina
Pérez Rodríguez, Aitzol
Cabello Beitia, Iván
Mateo Alesanco, Estibaliz
Sevillano Peña, Elena
Madariaga Torres, Lucila
Quindós Andrés, Guillermo
Eraso Barrio, María Elena
author Miranda Cadena, Katherine
author_facet Miranda Cadena, Katherine
Marcos Arias, Cristina
Pérez Rodríguez, Aitzol
Cabello Beitia, Iván
Mateo Alesanco, Estibaliz
Sevillano Peña, Elena
Madariaga Torres, Lucila
Quindós Andrés, Guillermo
Eraso Barrio, María Elena
author_role author
author2 Marcos Arias, Cristina
Pérez Rodríguez, Aitzol
Cabello Beitia, Iván
Mateo Alesanco, Estibaliz
Sevillano Peña, Elena
Madariaga Torres, Lucila
Quindós Andrés, Guillermo
Eraso Barrio, María Elena
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv candida
biofilm
citral
azole resistance
caenorhabditis elegans
gene expression
topic candida
biofilm
citral
azole resistance
caenorhabditis elegans
gene expression
description [EN] Background The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. Aim The aim of this study was to evaluate the antifungal activity of citral, a phytocompound present in lemongrass essential oil, in monotherapy and combined with fluconazole against azole-resistant Candida planktonic cells and biofilms. The effect of citral combined with fluconazole was also analysed with regard to the expression of fluconazole resistance-associated genes in Candida albicans and the effectiveness of the combination therapy in a Caenorhabditis elegans model of candidiasis. Results Citral reduced biofilm formation at initial stages and the metabolic activity of the mature biofilm. The combination of citral with fluconazole was synergistic, with a significant increase in the survival of C. elegans infected with Candida. RNA analysis revealed a reduction of the expression of the efflux pump encoded by MDR1, leading to a greater effect of fluconazole. Conclusion Citral in monotherapy and in combination with fluconazole could represent an interesting therapy to treat recalcitrant Candida infections associated to biofilms.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/56734
url http://hdl.handle.net/10810/56734
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/PID2020-117983RB-I00/
https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2045813
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/es/
Atribución-NoComercial 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/3.0/es/
Atribución-NoComercial 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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