Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]

Autonomous oscillatory dynamics are ubiquitous at every level in Biology. At the cellular level, one of the most relevant and well characterized examples of periodic behavior is the cyclic assembly and disassembly of actomyosin networks. In Drosophila, these oscillations induce the robust contractio...

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Detalhes bibliográficos
Autores: Hernández del Valle, Miguel, Valencia-Expósito, Andrea, Gorfinkiel, Nicole, Martín-Bermudo, María D., Míguez, David G.
Formato: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/331827
Acesso em linha:http://hdl.handle.net/10261/331827
Access Level:acceso abierto
Palavra-chave:Actomyosin
Coarse-grained
Proteins
Oscillations
Cytoskeleton
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spelling Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]Hernández del Valle, MiguelValencia-Expósito, AndreaGorfinkiel, NicoleMartín-Bermudo, María D.Míguez, David G.ActomyosinCoarse-grainedProteinsOscillationsCytoskeletonAutonomous oscillatory dynamics are ubiquitous at every level in Biology. At the cellular level, one of the most relevant and well characterized examples of periodic behavior is the cyclic assembly and disassembly of actomyosin networks. In Drosophila, these oscillations induce the robust contraction and expansion of individual cells required for correct dorsal closure, while in the follicular epithelium that surrounds the germline, periodic contractions of the basal actomyosin network are required for proper elongation of the egg chamber. While some studies suggest that actomyosin oscillations are driven by upstream signaling or mechanochemical features, we have recently proposed that they arise as a systems property from the competition between two well characterized features of the actomyosin machinery: 1) cooperative assembly of actin networks mediated by Actin crosslinker proteins and 2) tension-induced disassembly of actin networks mediated by myosin motors. Here, we perform experiments in amnioserosa and in the follicle cells of drosophila and simulations using a coarse-grained model of the actomyosin cortex to characterize the properties of the oscillations and how they depend on different features of the system. We also compare model and experiments to study the dynamics of actomyosin flows and the effect of mechanical coupling between cells in the tissue. In conclusion, our model is a powerful tool to study key features of actomyosin oscillations, from the effect of the individual components to network properties and finally supra-cellular organization of the oscillations at the tissue level.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionavihttp://hdl.handle.net/10261/331827reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésHernández del Valle, Miguel; Valencia-Expósito, Andrea; Gorfinkiel, Nicole; Martín-Bermudo, María D.; Míguez, David G. Analysis of actomyosin oscillatory dynamics using a coarse-grained model. http://dx.doi.org/10.3389/fphy.2022.881384 . http://hdl.handle.net/10261/281257https://doi.org/10.3389/fphy.2022.881384.s005Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3318272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
title Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
spellingShingle Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
Hernández del Valle, Miguel
Actomyosin
Coarse-grained
Proteins
Oscillations
Cytoskeleton
title_short Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
title_full Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
title_fullStr Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
title_full_unstemmed Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
title_sort Video5_Analysis of Actomyosin Oscillatory Dynamics Using a Coarse-Grained Model.AVI [Dataset]
dc.creator.none.fl_str_mv Hernández del Valle, Miguel
Valencia-Expósito, Andrea
Gorfinkiel, Nicole
Martín-Bermudo, María D.
Míguez, David G.
author Hernández del Valle, Miguel
author_facet Hernández del Valle, Miguel
Valencia-Expósito, Andrea
Gorfinkiel, Nicole
Martín-Bermudo, María D.
Míguez, David G.
author_role author
author2 Valencia-Expósito, Andrea
Gorfinkiel, Nicole
Martín-Bermudo, María D.
Míguez, David G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Actomyosin
Coarse-grained
Proteins
Oscillations
Cytoskeleton
topic Actomyosin
Coarse-grained
Proteins
Oscillations
Cytoskeleton
description Autonomous oscillatory dynamics are ubiquitous at every level in Biology. At the cellular level, one of the most relevant and well characterized examples of periodic behavior is the cyclic assembly and disassembly of actomyosin networks. In Drosophila, these oscillations induce the robust contraction and expansion of individual cells required for correct dorsal closure, while in the follicular epithelium that surrounds the germline, periodic contractions of the basal actomyosin network are required for proper elongation of the egg chamber. While some studies suggest that actomyosin oscillations are driven by upstream signaling or mechanochemical features, we have recently proposed that they arise as a systems property from the competition between two well characterized features of the actomyosin machinery: 1) cooperative assembly of actin networks mediated by Actin crosslinker proteins and 2) tension-induced disassembly of actin networks mediated by myosin motors. Here, we perform experiments in amnioserosa and in the follicle cells of drosophila and simulations using a coarse-grained model of the actomyosin cortex to characterize the properties of the oscillations and how they depend on different features of the system. We also compare model and experiments to study the dynamics of actomyosin flows and the effect of mechanical coupling between cells in the tissue. In conclusion, our model is a powerful tool to study key features of actomyosin oscillations, from the effect of the individual components to network properties and finally supra-cellular organization of the oscillations at the tissue level.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/331827
url http://hdl.handle.net/10261/331827
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Hernández del Valle, Miguel; Valencia-Expósito, Andrea; Gorfinkiel, Nicole; Martín-Bermudo, María D.; Míguez, David G. Analysis of actomyosin oscillatory dynamics using a coarse-grained model. http://dx.doi.org/10.3389/fphy.2022.881384 . http://hdl.handle.net/10261/281257
https://doi.org/10.3389/fphy.2022.881384.s005
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv avi
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418927063302144
score 15,812429

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