The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium
[EN] The four NGATHA genes (NGA) form a small subfamily within the large family of B3-domain transcription factors of Arabidopsis thaliana. NGA genes act redundantly to direct the development of the apical tissues of the gynoecium, the style, and the stigma. Previous studies indicate that NGA genes...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Data de publicação: | 2014 |
| País: | España |
| Recursos: | Universitat Politècnica de València (UPV) |
| Repositório: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglês |
| OAI Identifier: | oai:riunet.upv.es:10251/67957 |
| Acesso em linha: | https://riunet.upv.es/handle/10251/67957 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Gynoecium development Ngatha Auxin synthesis Auxin transport Auxin response factors |
| id |
ES_c4b6dc09c9ca1a653d7cfbbf1238c06a |
|---|---|
| oai_identifier_str |
oai:riunet.upv.es:10251/67957 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoeciumMartínez Fernández, IreneSanchis, SofíaMarini, NacieleNavarrete Gomez, Maria LuisaOliveira, Antonio C.Colombo, LuciaBalanzà, Vicente|||0000-0002-1761-1524Ballester Fuentes, PatriciaFERRANDIZ MAESTRE, CRISTINA|||0000-0002-2460-1068Gynoecium developmentNgathaAuxin synthesisAuxin transportAuxin response factors[EN] The four NGATHA genes (NGA) form a small subfamily within the large family of B3-domain transcription factors of Arabidopsis thaliana. NGA genes act redundantly to direct the development of the apical tissues of the gynoecium, the style, and the stigma. Previous studies indicate that NGA genes could exert this function at least partially by directing the synthesis of auxin at the distal end of the developing gynoecium through the upregulation of two different YUCCA genes, which encode flavin monooxygenases involved in auxin biosynthesis. We have compared three developing pistil transcriptome data sets from wildtype, nga quadruple mutants, and a 35S::NGA3 line. The differentially expressed genes showed a significant enrichment for auxin-related genes, supporting the idea of NGA genes as major regulators of auxin accumulation and distribution within the developing gynoecium. We have introduced reporter lines for several of these differentially expressed genes involved in synthesis, transport and response to auxin in NGA gain- and loss-of-function backgrounds. We present here a detailed map of the response of these reporters to NGA misregulation that could help to clarify the role of NGA in auxin-mediated gynoecium morphogenesis. Our data point to a very reduced auxin synthesis in the developing apical gynoecium of nga mutants, likely responsible for the lack of DR5rev::GFP reporter activity observed in these mutants. In addition, NGA altered activity affects the expression of protein kinases that regulate the cellular localization of auxin efflux regulators, and thus likely impact auxin transport. Finally, protein accumulation in pistils of several ARFs was differentially affected by nga mutations or NGA overexpression, suggesting that these accumulation patterns depend not only on auxin distribution but could be also regulated by transcriptional networks involving NGA factors.This work was supported by the Spanish Ministerio de Ciencia e Innovacion (grant no. BIO2009–09920 to Cristina Ferrándiz), the Fondazione Cariplo (grant “Fruitalia” to Lucia Colombo and Cristina Ferrándiz), and the European Union (grant no. FP7–PEOPLE–PIRSES–2009–247589 to Lucia Colombo, Cristina Ferrándiz, Antonio C. Oliveira).Frontiers MediaInstituto Universitario Mixto de Biología Molecular y Celular de PlantasEuropean CommissionMinisterio de Ciencia e InnovaciónFondazione CariploRepositorio Institucional de la Universitat Politècnica de València Riunet20142014-05-21journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/67957reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 FP7 247589Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2009-09920 Conservacion De Los Modelos Geneticos Que Explican La Morfogenesis Del Carpelo Y El Fruto En Las Eudicotiledoneasopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/679572026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| title |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| spellingShingle |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium Martínez Fernández, Irene Gynoecium development Ngatha Auxin synthesis Auxin transport Auxin response factors |
| title_short |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| title_full |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| title_fullStr |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| title_full_unstemmed |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| title_sort |
The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium |
| dc.creator.none.fl_str_mv |
Martínez Fernández, Irene Sanchis, Sofía Marini, Naciele Navarrete Gomez, Maria Luisa Oliveira, Antonio C. Colombo, Lucia Balanzà, Vicente|||0000-0002-1761-1524 Ballester Fuentes, Patricia FERRANDIZ MAESTRE, CRISTINA|||0000-0002-2460-1068 |
| author |
Martínez Fernández, Irene |
| author_facet |
Martínez Fernández, Irene Sanchis, Sofía Marini, Naciele Navarrete Gomez, Maria Luisa Oliveira, Antonio C. Colombo, Lucia Balanzà, Vicente|||0000-0002-1761-1524 Ballester Fuentes, Patricia FERRANDIZ MAESTRE, CRISTINA|||0000-0002-2460-1068 |
| author_role |
author |
| author2 |
Sanchis, Sofía Marini, Naciele Navarrete Gomez, Maria Luisa Oliveira, Antonio C. Colombo, Lucia Balanzà, Vicente|||0000-0002-1761-1524 Ballester Fuentes, Patricia FERRANDIZ MAESTRE, CRISTINA|||0000-0002-2460-1068 |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto Universitario Mixto de Biología Molecular y Celular de Plantas European Commission Ministerio de Ciencia e Innovación Fondazione Cariplo Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Gynoecium development Ngatha Auxin synthesis Auxin transport Auxin response factors |
| topic |
Gynoecium development Ngatha Auxin synthesis Auxin transport Auxin response factors |
| description |
[EN] The four NGATHA genes (NGA) form a small subfamily within the large family of B3-domain transcription factors of Arabidopsis thaliana. NGA genes act redundantly to direct the development of the apical tissues of the gynoecium, the style, and the stigma. Previous studies indicate that NGA genes could exert this function at least partially by directing the synthesis of auxin at the distal end of the developing gynoecium through the upregulation of two different YUCCA genes, which encode flavin monooxygenases involved in auxin biosynthesis. We have compared three developing pistil transcriptome data sets from wildtype, nga quadruple mutants, and a 35S::NGA3 line. The differentially expressed genes showed a significant enrichment for auxin-related genes, supporting the idea of NGA genes as major regulators of auxin accumulation and distribution within the developing gynoecium. We have introduced reporter lines for several of these differentially expressed genes involved in synthesis, transport and response to auxin in NGA gain- and loss-of-function backgrounds. We present here a detailed map of the response of these reporters to NGA misregulation that could help to clarify the role of NGA in auxin-mediated gynoecium morphogenesis. Our data point to a very reduced auxin synthesis in the developing apical gynoecium of nga mutants, likely responsible for the lack of DR5rev::GFP reporter activity observed in these mutants. In addition, NGA altered activity affects the expression of protein kinases that regulate the cellular localization of auxin efflux regulators, and thus likely impact auxin transport. Finally, protein accumulation in pistils of several ARFs was differentially affected by nga mutations or NGA overexpression, suggesting that these accumulation patterns depend not only on auxin distribution but could be also regulated by transcriptional networks involving NGA factors. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2014-05-21 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/67957 |
| url |
https://riunet.upv.es/handle/10251/67957 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 FP7 247589 Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2009-09920 Conservacion De Los Modelos Geneticos Que Explican La Morfogenesis Del Carpelo Y El Fruto En Las Eudicotiledoneas |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
| instname_str |
Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869418925929791488 |
| score |
15,301603 |