GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage

Idiopathic pulmonary fibrosis is a lethal lung fibrotic disease, associated with aging with a mean survival of 2-5 years and no curative treatment. The GSE4 peptide is able to rescue cells from senescence, DNA and oxidative damage, inflammation, and induces telomerase activity. Here, we investigated...

ver descrição completa

Detalhes bibliográficos
Autores: Pintado-Berninches, Laura, Montes-Worboys, Ana, Manguan-García, Cristina, Arias-Salgado, Elena G., Serrano, Adela, Fernandez-Varas, Beatriz, Guerrero-López, Rosa, Iarriccio, Laura, Planas, Lurdes, Guenechea, Guillermo, Cortijo, Julio, Sastre, Leandro, Molina-Molina, Maria, Perona, Rosario
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
Repositorio:Docu-menta. Repositorio Institucional del CIEMAT
Idioma:inglés
OAI Identifier:oai:dnet:documenta___::08b5d3fd5893bc700a5feba4bad42e78
Acesso em linha:https://hdl.handle.net/20.500.14855/4404
Access Level:acceso abierto
id ES_c4b4435d552ff545dba8a5149b7be374
oai_identifier_str oai:dnet:documenta___::08b5d3fd5893bc700a5feba4bad42e78
network_acronym_str ES
network_name_str España
repository_id_str
spelling GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damagePintado-Berninches, LauraMontes-Worboys, AnaManguan-García, CristinaArias-Salgado, Elena G.Serrano, AdelaFernandez-Varas, BeatrizGuerrero-López, RosaIarriccio, LauraPlanas, LurdesGuenechea, GuillermoCortijo, JulioSastre, LeandroMolina-Molina, MariaPerona, RosarioIdiopathic pulmonary fibrosis is a lethal lung fibrotic disease, associated with aging with a mean survival of 2-5 years and no curative treatment. The GSE4 peptide is able to rescue cells from senescence, DNA and oxidative damage, inflammation, and induces telomerase activity. Here, we investigated the protective effect of GSE4 expression in vitro in rat alveolar epithelial cells (AECs), and in vivo in a bleomycin model of lung fibrosis. Bleomycin-injured rat AECs, expressing GSE4 or treated with GSE4-PLGA/PEI nanoparticles showed an increase of telomerase activity, decreased DNA damage, and decreased expression of IL6 and cleaved-caspase 3. In addition, these cells showed an inhibition in expression of fibrotic markers induced by TGF-βsuch as collagen-I and III among others. Furthermore, treatment with GSE4-PLGA/PEI nanoparticles in a rat model of bleomycin-induced fibrosis, increased telomerase activity and decreased DNA damage in proSP-C cells. Both in preventive and therapeutic protocols GSE4-PLGA/ PEI nanoparticles prevented and attenuated lung damage monitored by SPECT-CT and inhibited collagen deposition. Lungs of rats treated with bleomycin and GSE4-PLGA/PEI nanoparticles showed reduced expression of α-SMA and pro-inflammatory cytokines, increased number of pro-SPC- Multicellular structures and increased DNA synthesis in proSP-C cells, indicating therapeutic efficacy of GSE4-nanoparticles in experimental lung fibrosis and a possible curative treatment for lung fibrotic patients.20252025-01-2820252025-01-2820252025-01-28journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14855/4404reponame:Docu-menta. Repositorio Institucional del CIEMATinstname:Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dnet:documenta___::08b5d3fd5893bc700a5feba4bad42e782026-06-18T12:40:47Z
dc.title.none.fl_str_mv GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
title GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
spellingShingle GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
Pintado-Berninches, Laura
title_short GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
title_full GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
title_fullStr GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
title_full_unstemmed GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
title_sort GSE4-loaded nanoparticles a potential therapy for lung fibrosis that enhances pneumocyte growth, reduces apoptosis and DNA damage
dc.creator.none.fl_str_mv Pintado-Berninches, Laura
Montes-Worboys, Ana
Manguan-García, Cristina
Arias-Salgado, Elena G.
Serrano, Adela
Fernandez-Varas, Beatriz
Guerrero-López, Rosa
Iarriccio, Laura
Planas, Lurdes
Guenechea, Guillermo
Cortijo, Julio
Sastre, Leandro
Molina-Molina, Maria
Perona, Rosario
author Pintado-Berninches, Laura
author_facet Pintado-Berninches, Laura
Montes-Worboys, Ana
Manguan-García, Cristina
Arias-Salgado, Elena G.
Serrano, Adela
Fernandez-Varas, Beatriz
Guerrero-López, Rosa
Iarriccio, Laura
Planas, Lurdes
Guenechea, Guillermo
Cortijo, Julio
Sastre, Leandro
Molina-Molina, Maria
Perona, Rosario
author_role author
author2 Montes-Worboys, Ana
Manguan-García, Cristina
Arias-Salgado, Elena G.
Serrano, Adela
Fernandez-Varas, Beatriz
Guerrero-López, Rosa
Iarriccio, Laura
Planas, Lurdes
Guenechea, Guillermo
Cortijo, Julio
Sastre, Leandro
Molina-Molina, Maria
Perona, Rosario
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
description Idiopathic pulmonary fibrosis is a lethal lung fibrotic disease, associated with aging with a mean survival of 2-5 years and no curative treatment. The GSE4 peptide is able to rescue cells from senescence, DNA and oxidative damage, inflammation, and induces telomerase activity. Here, we investigated the protective effect of GSE4 expression in vitro in rat alveolar epithelial cells (AECs), and in vivo in a bleomycin model of lung fibrosis. Bleomycin-injured rat AECs, expressing GSE4 or treated with GSE4-PLGA/PEI nanoparticles showed an increase of telomerase activity, decreased DNA damage, and decreased expression of IL6 and cleaved-caspase 3. In addition, these cells showed an inhibition in expression of fibrotic markers induced by TGF-βsuch as collagen-I and III among others. Furthermore, treatment with GSE4-PLGA/PEI nanoparticles in a rat model of bleomycin-induced fibrosis, increased telomerase activity and decreased DNA damage in proSP-C cells. Both in preventive and therapeutic protocols GSE4-PLGA/ PEI nanoparticles prevented and attenuated lung damage monitored by SPECT-CT and inhibited collagen deposition. Lungs of rats treated with bleomycin and GSE4-PLGA/PEI nanoparticles showed reduced expression of α-SMA and pro-inflammatory cytokines, increased number of pro-SPC- Multicellular structures and increased DNA synthesis in proSP-C cells, indicating therapeutic efficacy of GSE4-nanoparticles in experimental lung fibrosis and a possible curative treatment for lung fibrotic patients.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-28
2025
2025-01-28
2025
2025-01-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14855/4404
url https://hdl.handle.net/20.500.14855/4404
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docu-menta. Repositorio Institucional del CIEMAT
instname:Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
instname_str Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
reponame_str Docu-menta. Repositorio Institucional del CIEMAT
collection Docu-menta. Repositorio Institucional del CIEMAT
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418924823543808
score 15,811543

Registos relacionados