Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands

The attachment of a variety of Lactobacilli to the mucosal surfaces is accomplished through the interaction of OppA, a superficial bacterial protein also involved in oligopeptide internalization, and the glycosaminoglycan moiety of the proteoglycans that form the epithelial cell glycocalyx. Upon the...

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Autores: Martín, Carla, Fernández Vega, Iván|||0000-0002-3141-6493, Suárez, Juan E, Quirós Fernández, Luis Manuel|||0000-0003-3505-3762
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad de Oviedo (UNIOVI)
Repositorio:RUO. Repositorio Institucional de la Universidad de Oviedo
Idioma:inglés
OAI Identifier:oai:digibuo.uniovi.es:10651/76209
Acesso em linha:https://hdl.handle.net/10651/76209
https://dx.doi.org/10.3389/fimmu.2019.03019
Access Level:acceso abierto
Palavra-chave:Lactobacillus salivarius
glycosaminoglycan
heparan sulfate
proteoglycan
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spelling Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their LigandsMartín, CarlaFernández Vega, Iván|||0000-0002-3141-6493Suárez, Juan EQuirós Fernández, Luis Manuel|||0000-0003-3505-3762Lactobacillus salivariusglycosaminoglycanheparan sulfateproteoglycanThe attachment of a variety of Lactobacilli to the mucosal surfaces is accomplished through the interaction of OppA, a superficial bacterial protein also involved in oligopeptide internalization, and the glycosaminoglycan moiety of the proteoglycans that form the epithelial cell glycocalyx. Upon the interaction of the vaginal isolate Lactobacillus salivarius Lv72 and HeLa cell cultures, the expression of oppA increased more than 50-fold over the following 30 min, with the overexpression enduring, albeit at a lower rate, for up to 24 h. Conversely, transcriptional analysis of 62 genes involved in proteoglycan biosynthesis revealed generalized repression of genes whose products catalyze different steps of the whole pathway. This led to decreases in the superficial concentration of heparan (60%) and chondroitin sulfate (40%), although the molecular masses of these glycosaminoglycans were higher than those of the control cultures. Despite this lowering in the concentration of the receptor, attachment of the Lactobacilli proceeded, and completely overlaid the underlying HeLa cell culture.This work was supported by CICYT grant AGL2010-15097 from the Ministry of Science and Technology (Spain), and the Programa de Ciencia, Tecnología e Innovación of the Principado de Asturias for 2013–2017 (GRUPIN 14-139 to JS and GRUPIN 14-141 to LQ). CM held a scholarship within this project.20202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttps://hdl.handle.net/10651/76209https://dx.doi.org/10.3389/fimmu.2019.03019reponame:RUO. Repositorio Institucional de la Universidad de Oviedoinstname:Universidad de Oviedo (UNIOVI)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:digibuo.uniovi.es:10651/762092026-06-07T06:38:51Z
dc.title.none.fl_str_mv Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
title Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
spellingShingle Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
Martín, Carla
Lactobacillus salivarius
glycosaminoglycan
heparan sulfate
proteoglycan
title_short Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
title_full Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
title_fullStr Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
title_full_unstemmed Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
title_sort Adherence of Lactobacillus salivarius to HeLa Cells Promotes Changes in the Expression of the Genes Involved in Biosynthesis of Their Ligands
dc.creator.none.fl_str_mv Martín, Carla
Fernández Vega, Iván|||0000-0002-3141-6493
Suárez, Juan E
Quirós Fernández, Luis Manuel|||0000-0003-3505-3762
author Martín, Carla
author_facet Martín, Carla
Fernández Vega, Iván|||0000-0002-3141-6493
Suárez, Juan E
Quirós Fernández, Luis Manuel|||0000-0003-3505-3762
author_role author
author2 Fernández Vega, Iván|||0000-0002-3141-6493
Suárez, Juan E
Quirós Fernández, Luis Manuel|||0000-0003-3505-3762
author2_role author
author
author
dc.subject.none.fl_str_mv Lactobacillus salivarius
glycosaminoglycan
heparan sulfate
proteoglycan
topic Lactobacillus salivarius
glycosaminoglycan
heparan sulfate
proteoglycan
description The attachment of a variety of Lactobacilli to the mucosal surfaces is accomplished through the interaction of OppA, a superficial bacterial protein also involved in oligopeptide internalization, and the glycosaminoglycan moiety of the proteoglycans that form the epithelial cell glycocalyx. Upon the interaction of the vaginal isolate Lactobacillus salivarius Lv72 and HeLa cell cultures, the expression of oppA increased more than 50-fold over the following 30 min, with the overexpression enduring, albeit at a lower rate, for up to 24 h. Conversely, transcriptional analysis of 62 genes involved in proteoglycan biosynthesis revealed generalized repression of genes whose products catalyze different steps of the whole pathway. This led to decreases in the superficial concentration of heparan (60%) and chondroitin sulfate (40%), although the molecular masses of these glycosaminoglycans were higher than those of the control cultures. Despite this lowering in the concentration of the receptor, attachment of the Lactobacilli proceeded, and completely overlaid the underlying HeLa cell culture.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10651/76209
https://dx.doi.org/10.3389/fimmu.2019.03019
url https://hdl.handle.net/10651/76209
https://dx.doi.org/10.3389/fimmu.2019.03019
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUO. Repositorio Institucional de la Universidad de Oviedo
instname:Universidad de Oviedo (UNIOVI)
instname_str Universidad de Oviedo (UNIOVI)
reponame_str RUO. Repositorio Institucional de la Universidad de Oviedo
collection RUO. Repositorio Institucional de la Universidad de Oviedo
repository.name.fl_str_mv
repository.mail.fl_str_mv
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