Direct and label-free detection of the human growth hormone in urine by an ultrasensitive bimodal waveguide biosensor

A label-free interferometric transducer showing a theoretical detection limit for homogeneous sensing of 5 × 10⁻⁸ RIU, being equivalent to a protein mass coverage resolution of 2.8 fg mm⁻², is used to develop a high sensitive biosensor for protein detection. The extreme sensitivity of this transduce...

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Detalles Bibliográficos
Autores: González-Guerrero, Ana Belén|||0000-0002-4871-9642, Maldonado, Jesús, Dante, Stefania|||0000-0003-0215-6896, Grajales García, Daniel|||0000-0003-0898-4975, Lechuga, Laura M.|||0000-0001-5187-5358
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:195672
Acceso en línea:https://ddd.uab.cat/record/195672
https://dx.doi.org/urn:doi:10.1002/jbio.201600154
Access Level:acceso abierto
Palabra clave:BiMW interferometer
Silicon nanowaveguides
Label-free biosensing
Hgh detection
Pg/ml detection range
Descripción
Sumario:A label-free interferometric transducer showing a theoretical detection limit for homogeneous sensing of 5 × 10⁻⁸ RIU, being equivalent to a protein mass coverage resolution of 2.8 fg mm⁻², is used to develop a high sensitive biosensor for protein detection. The extreme sensitivity of this transducer combined with a selective bioreceptor layer enables the direct evaluation of the human growth hormone (hGH) in undiluted urine matrix in the 10 pg mL⁻¹ range. (Figure presented.).