Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients

This work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, in addition to increasing the amount of body fat, will also modify the lipid composition of membranes in cells other than adipocytes. Forty-n...

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Autores: Sot, Jesús, García-Arribas, Aritz B., Abad, Beatriz, Arranz, Sara, Portune, Kevin, Andrade, Fernando, Martín-Nieto, Alicia, Velasco, Olaia, Arana, Eunate, Tueros, Itziar, Ferreri, Carla, Gaztambide, Sonia, Goñi, Félix M., Castaño, Luis, Alonso, Alicia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/296798
Acesso em linha:http://hdl.handle.net/10261/296798
Access Level:acceso abierto
Palavra-chave:Obesity
Cell membrane physical properties
Membrane fluidity
Fluorescence polarization
Atomic force microscopy
Membrane breakthrough force
Lipidomics
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spelling Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese PatientsSot, JesúsGarcía-Arribas, Aritz B.Abad, BeatrizArranz, SaraPortune, KevinAndrade, FernandoMartín-Nieto, AliciaVelasco, OlaiaArana, EunateTueros, ItziarFerreri, CarlaGaztambide, SoniaGoñi, Félix M.Castaño, LuisAlonso, AliciaObesityCell membrane physical propertiesMembrane fluidityFluorescence polarizationAtomic force microscopyMembrane breakthrough forceLipidomicsThis work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, in addition to increasing the amount of body fat, will also modify the lipid composition of membranes in cells other than adipocytes. Forty-nine control volunteers (16 male, 33 female, BMI 21.8 ± 5.6 and 21.5 ± 4.2 kg/m2, respectively) and 52 obese subjects (16 male and 36 female, BMI 38.2± 11.0 and 40.7 ± 8.7 kg/m2, respectively) were examined. The two physical techniques applied were atomic force microscopy (AFM) in the force spectroscopy mode, which allows the micromechanical measurement of penetration forces, and fluorescence anisotropy of trimethylammonium diphenylhexatriene (TMA-DPH), which provides information on lipid order at the membrane polar–nonpolar interface. These techniques, in combination with lipidomic studies, revealed a decreased rigidity in the interfacial region of the RBC membranes of obese as compared to control patients, related to parallel changes in lipid composition. Lipidomic data show an increase in the cholesterol/phospholipid mole ratio and a decrease in sphingomyelin contents in obese membranes. ω-3 fatty acids (e.g., docosahexaenoic acid) appear to be less prevalent in obese patient RBCs, and this is the case for both the global fatty acid distribution and for the individual major lipids in the membrane phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS). Moreover, some ω-6 fatty acids (e.g., arachidonic acid) are increased in obese patient RBCs. The switch from ω-3 to ω-6 lipids in obese subjects could be a major factor explaining the higher interfacial fluidity in obese patient RBC membranes.This work was supported in part by the Basque Government Department of Economic Development, grant No. KK-2019/00028 (OBINTER); the Basque Government Department of Education, grants No. IT1264-19, IT1281-19, IT1270-19, and IT1625-22; the Basque Government Department of Health, grants No. 2019-222030, 2020-333023; Fundación Ramón Areces; and by Centre for the Development of Industrial Technology (CDTI) of the Spanish Ministry of Science and Innovation under the grant agreement: TECNOMIFOOD project (CER-20191010) and Basque Government: IT1625-22.Peer reviewedMultidisciplinary Digital Publishing InstituteEusko JaurlaritzaFundación Ramón ArecesMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Centro para el Desarrollo Tecnológico Industrial (España)Sot, Jesús [0000-0001-9508-6596]García Arribas, Aritz B. [0000-0003-1866-6647]Arranz, Sara [0000-0003-2397-5145]Tueros, Itziar [0000-0002-7609-5435]Alonso, Alicia [0000-0002-2730-7470]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/296798reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at 10.3390/ijms23031920https://doi.org/10.3390/ijms23031920Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2967982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
title Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
spellingShingle Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
Sot, Jesús
Obesity
Cell membrane physical properties
Membrane fluidity
Fluorescence polarization
Atomic force microscopy
Membrane breakthrough force
Lipidomics
title_short Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
title_full Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
title_fullStr Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
title_full_unstemmed Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
title_sort Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
dc.creator.none.fl_str_mv Sot, Jesús
García-Arribas, Aritz B.
Abad, Beatriz
Arranz, Sara
Portune, Kevin
Andrade, Fernando
Martín-Nieto, Alicia
Velasco, Olaia
Arana, Eunate
Tueros, Itziar
Ferreri, Carla
Gaztambide, Sonia
Goñi, Félix M.
Castaño, Luis
Alonso, Alicia
author Sot, Jesús
author_facet Sot, Jesús
García-Arribas, Aritz B.
Abad, Beatriz
Arranz, Sara
Portune, Kevin
Andrade, Fernando
Martín-Nieto, Alicia
Velasco, Olaia
Arana, Eunate
Tueros, Itziar
Ferreri, Carla
Gaztambide, Sonia
Goñi, Félix M.
Castaño, Luis
Alonso, Alicia
author_role author
author2 García-Arribas, Aritz B.
Abad, Beatriz
Arranz, Sara
Portune, Kevin
Andrade, Fernando
Martín-Nieto, Alicia
Velasco, Olaia
Arana, Eunate
Tueros, Itziar
Ferreri, Carla
Gaztambide, Sonia
Goñi, Félix M.
Castaño, Luis
Alonso, Alicia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Eusko Jaurlaritza
Fundación Ramón Areces
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Centro para el Desarrollo Tecnológico Industrial (España)
Sot, Jesús [0000-0001-9508-6596]
García Arribas, Aritz B. [0000-0003-1866-6647]
Arranz, Sara [0000-0003-2397-5145]
Tueros, Itziar [0000-0002-7609-5435]
Alonso, Alicia [0000-0002-2730-7470]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Obesity
Cell membrane physical properties
Membrane fluidity
Fluorescence polarization
Atomic force microscopy
Membrane breakthrough force
Lipidomics
topic Obesity
Cell membrane physical properties
Membrane fluidity
Fluorescence polarization
Atomic force microscopy
Membrane breakthrough force
Lipidomics
description This work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, in addition to increasing the amount of body fat, will also modify the lipid composition of membranes in cells other than adipocytes. Forty-nine control volunteers (16 male, 33 female, BMI 21.8 ± 5.6 and 21.5 ± 4.2 kg/m2, respectively) and 52 obese subjects (16 male and 36 female, BMI 38.2± 11.0 and 40.7 ± 8.7 kg/m2, respectively) were examined. The two physical techniques applied were atomic force microscopy (AFM) in the force spectroscopy mode, which allows the micromechanical measurement of penetration forces, and fluorescence anisotropy of trimethylammonium diphenylhexatriene (TMA-DPH), which provides information on lipid order at the membrane polar–nonpolar interface. These techniques, in combination with lipidomic studies, revealed a decreased rigidity in the interfacial region of the RBC membranes of obese as compared to control patients, related to parallel changes in lipid composition. Lipidomic data show an increase in the cholesterol/phospholipid mole ratio and a decrease in sphingomyelin contents in obese membranes. ω-3 fatty acids (e.g., docosahexaenoic acid) appear to be less prevalent in obese patient RBCs, and this is the case for both the global fatty acid distribution and for the individual major lipids in the membrane phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS). Moreover, some ω-6 fatty acids (e.g., arachidonic acid) are increased in obese patient RBCs. The switch from ω-3 to ω-6 lipids in obese subjects could be a major factor explaining the higher interfacial fluidity in obese patient RBC membranes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/296798
url http://hdl.handle.net/10261/296798
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.3390/ijms23031920
https://doi.org/10.3390/ijms23031920
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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