Engineering the halophilic bacterium Halomonas elongata to produce β-carotene
Engineering halophilic bacteria to produce carotenoids is a subject of great scientific and commercial interest, as carotenoids are desirable products used as additives and colorants in the food industry, with β-carotene the most prominent. With this target, we expressed the β-carotene biosynthetic...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2007 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/165140 |
| Acceso en línea: | https://hdl.handle.net/11441/165140 https://doi.org/10.1007/s00253-007-1195-2 |
| Access Level: | acceso abierto |
| Palabra clave: | Carotene Carotenoid Engineering Halomonas Halophilic |
| Sumario: | Engineering halophilic bacteria to produce carotenoids is a subject of great scientific and commercial interest, as carotenoids are desirable products used as additives and colorants in the food industry, with β-carotene the most prominent. With this target, we expressed the β-carotene biosynthetic genes crtE, crtY, crtI, and crtB from Pantoea agglomerans and the cDNA encoding isopentenyl pyrophosphate isomerase from Haematococcus pluvialis in the halophilic bacterium Halomonas elongata obtaining a strain able to produce practically pure β-carotene. Reverse transcription-polymerase chain reaction analysis showed crtY, crtI, and crtB heterologous expression in a selected exconjugant of H. elongata. Biosynthesis of β-carotene was dependent on NaCl concentration in the culture medium, with the highest production (560 μg per g of dry weight) in 2% NaCl. On the contrary, no β-carotene was detected in 15% NaCl. Successful construction of the β-carotene biosynthetic pathway in H. elongata opens the possibility of engineering halophilic bacteria for carotenoid production. |
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