Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance

Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds – commercial examples include compounds such as paclitaxel. The major con...

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Autores: Häkkinen, Suvi T., Moyano Claramunt, Elisabet, Cusidó Vidal, Rosa M., Oksman-Caldentey, Kirsi-Marja
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/28255
Acesso em linha:http://hdl.handle.net/10230/28255
http://dx.doi.org/10.3389/fpls.2016.01486
Access Level:acceso abierto
Palavra-chave:Plant cell culture
Hairy roots
Hyoscyamine 6β-hydroxylase
Scopolamine
Stability
Cryopreservation
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spelling Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years MaintenanceHäkkinen, Suvi T.Moyano Claramunt, ElisabetCusidó Vidal, Rosa M.Oksman-Caldentey, Kirsi-MarjaPlant cell cultureHairy rootsHyoscyamine 6β-hydroxylaseScopolamineStabilityCryopreservationPlants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds – commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells. Although genetic and metabolic stability has often been connected to transgenic hairy roots, there are only few reports on how a very long-term subculturing effects on the production capacity of hairy roots. In this study, hairy roots producing high tropane alkaloid levels were subjected to 16-year follow-up in relation to genetic and metabolic stability. Cryopreservation method for hairy roots of Hyoscyamus muticus was developed to replace laborious subculturing, and although the post-thaw recovery rates remained low, the expression of transgene remained unaltered in cryopreserved roots. It was shown that although displaying some fluctuation in the metabolite yields, even an exceedingly long-term subculturing was successfully applied without significant loss of metabolic activity.Frontiers201720172016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/28255http://dx.doi.org/10.3389/fpls.2016.01486reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in Plant Science. 2016;7:1486© 2016 Häkkinen, Moyano, Cusidó and Oksman-Caldentey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/282552026-05-29T05:05:01Z
dc.title.none.fl_str_mv Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
title Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
spellingShingle Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
Häkkinen, Suvi T.
Plant cell culture
Hairy roots
Hyoscyamine 6β-hydroxylase
Scopolamine
Stability
Cryopreservation
title_short Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
title_full Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
title_fullStr Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
title_full_unstemmed Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
title_sort Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance
dc.creator.none.fl_str_mv Häkkinen, Suvi T.
Moyano Claramunt, Elisabet
Cusidó Vidal, Rosa M.
Oksman-Caldentey, Kirsi-Marja
author Häkkinen, Suvi T.
author_facet Häkkinen, Suvi T.
Moyano Claramunt, Elisabet
Cusidó Vidal, Rosa M.
Oksman-Caldentey, Kirsi-Marja
author_role author
author2 Moyano Claramunt, Elisabet
Cusidó Vidal, Rosa M.
Oksman-Caldentey, Kirsi-Marja
author2_role author
author
author
dc.subject.none.fl_str_mv Plant cell culture
Hairy roots
Hyoscyamine 6β-hydroxylase
Scopolamine
Stability
Cryopreservation
topic Plant cell culture
Hairy roots
Hyoscyamine 6β-hydroxylase
Scopolamine
Stability
Cryopreservation
description Plants remain a major source of new drugs, leads and fine chemicals. Cell cultures deriving from plants offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds – commercial examples include compounds such as paclitaxel. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells. Although genetic and metabolic stability has often been connected to transgenic hairy roots, there are only few reports on how a very long-term subculturing effects on the production capacity of hairy roots. In this study, hairy roots producing high tropane alkaloid levels were subjected to 16-year follow-up in relation to genetic and metabolic stability. Cryopreservation method for hairy roots of Hyoscyamus muticus was developed to replace laborious subculturing, and although the post-thaw recovery rates remained low, the expression of transgene remained unaltered in cryopreserved roots. It was shown that although displaying some fluctuation in the metabolite yields, even an exceedingly long-term subculturing was successfully applied without significant loss of metabolic activity.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/28255
http://dx.doi.org/10.3389/fpls.2016.01486
url http://hdl.handle.net/10230/28255
http://dx.doi.org/10.3389/fpls.2016.01486
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Plant Science. 2016;7:1486
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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