Two-dimensional gel analysis of proteins in the Drosophila wing imaginal disc mutants fat and lethal (2) giant discs
High-resolution two-dimensional (2D) gel electrophoresis coupled with computer analysis has been used to construct a quantitative protein database ofDrosophilamature wing imaginal discs. The level of expression for all of the detected proteins has been quantitatively determined. This database has be...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 1998 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/48213 |
| Acceso en línea: | http://hdl.handle.net/10261/48213 |
| Access Level: | acceso embargado |
| Palabra clave: | Wing imaginal discs Drosophila Tumor suppressor 2D gels Database |
| Sumario: | High-resolution two-dimensional (2D) gel electrophoresis coupled with computer analysis has been used to construct a quantitative protein database ofDrosophilamature wing imaginal discs. The level of expression for all of the detected proteins has been quantitatively determined. This database has been used to evaluate changes in the patterns of protein synthesis in wing imaginal discs from twoDrosophila melanogastermutants with abnormal wing disc development:fat(ft) and two different alleles oflethal (2) giant disc(l(2)gd). Patterns of pulse-labeled proteins of the different mutants show variations in both qualitative and quantitative parameters of synthesis. In this comparison we have detected specific sets of protein changes characteristic of both alleles of the same locus and a set of protein changes common to both loci. How the abnormal expression of these proteins relates to the abnormal process of mutant hyperplasia is discussed. |
|---|