Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo

The ectoenzymes CD39 and CD73 regulate the purinergic signaling through the hydrolysis of adenosine triphosphate (ATP)/ADP to AMP and to adenosine (Ado), respectively. This shifts the pro-inflammatory milieu induced by extracellular ATP to the anti- inflammatory regulation by Ado. Mesenchymal stem c...

Descripción completa

Detalles Bibliográficos
Autores: Monguió-Tortajada, Marta|||0000-0003-2125-0810, Rudilla, F.., Gálvez-Montón, Carolina|||0000-0003-2254-9371, Franquesa, Marcella|||0000-0002-1287-8908, Bayés-Genís, Antoni|||0000-0002-3044-197X, Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:196945
Acceso en línea:https://ddd.uab.cat/record/196945
https://dx.doi.org/urn:doi:10.3389/fimmu.2017.01577
Access Level:acceso abierto
Palabra clave:CD73
Adenosine
Immunomodulation
Mesenchymal stem cell
Ectonucleotidase
Myocardial infarction
Purigernic signaling
Regeneration
id ES_c3d9f00a9360ec68d0a8edd34c19da8f
oai_identifier_str oai:ddd.uab.cat:196945
network_acronym_str ES
network_name_str España
repository_id_str
spelling Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivoMonguió-Tortajada, Marta|||0000-0003-2125-0810Rudilla, F..Gálvez-Montón, Carolina|||0000-0003-2254-9371Franquesa, Marcella|||0000-0002-1287-8908Bayés-Genís, Antoni|||0000-0002-3044-197XBorràs i Serres, Francesc Enric|||0000-0003-4038-1912CD73AdenosineImmunomodulationMesenchymal stem cellEctonucleotidaseMyocardial infarctionPurigernic signalingRegenerationThe ectoenzymes CD39 and CD73 regulate the purinergic signaling through the hydrolysis of adenosine triphosphate (ATP)/ADP to AMP and to adenosine (Ado), respectively. This shifts the pro-inflammatory milieu induced by extracellular ATP to the anti- inflammatory regulation by Ado. Mesenchymal stem cells (MSCs) have potent immunomodulatory capabilities, including monocyte modulation toward an anti-inflammatory phenotype aiding tissue repair. In vitro, we observed that human cardiac adipose tissue-derived MSCs (cATMSCs) and umbilical cord MSCs similarly polarize monocytes toward a regulatory M2 phenotype, which maintained the expression of CD39 and induced expression of CD73 in a cell contact dependent fashion, correlating with increased functional activity. In addition, the local treatment with porcine cATMSCs using an engineered bioactive graft promoted the in vivo CD73 expression on host monocytes in a swine model of myocardial infarction. Our results suggest the upregulation of ectonucleotidases on MSC-conditioned monocytes as an effective mechanism to amplify the long-lasting immunomodulatory and healing effects of MSCs delivery. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/196945https://dx.doi.org/urn:doi:10.3389/fimmu.2017.01577reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI14/01682Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-804Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-699Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 REDinREN/16/0009Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2014-59892-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00403Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 RD16/00111/0006Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014FIB00649open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1969452026-06-06T12:50:31Z
dc.title.none.fl_str_mv Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
title Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
spellingShingle Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
Monguió-Tortajada, Marta|||0000-0003-2125-0810
CD73
Adenosine
Immunomodulation
Mesenchymal stem cell
Ectonucleotidase
Myocardial infarction
Purigernic signaling
Regeneration
title_short Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
title_full Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
title_fullStr Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
title_full_unstemmed Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
title_sort Mesenchymal stem cells induce expression of cD73 in human monocytes in vitro and in a swine model of myocardial infarction in vivo
dc.creator.none.fl_str_mv Monguió-Tortajada, Marta|||0000-0003-2125-0810
Rudilla, F..
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Franquesa, Marcella|||0000-0002-1287-8908
Bayés-Genís, Antoni|||0000-0002-3044-197X
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
author Monguió-Tortajada, Marta|||0000-0003-2125-0810
author_facet Monguió-Tortajada, Marta|||0000-0003-2125-0810
Rudilla, F..
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Franquesa, Marcella|||0000-0002-1287-8908
Bayés-Genís, Antoni|||0000-0002-3044-197X
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
author_role author
author2 Rudilla, F..
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Franquesa, Marcella|||0000-0002-1287-8908
Bayés-Genís, Antoni|||0000-0002-3044-197X
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CD73
Adenosine
Immunomodulation
Mesenchymal stem cell
Ectonucleotidase
Myocardial infarction
Purigernic signaling
Regeneration
topic CD73
Adenosine
Immunomodulation
Mesenchymal stem cell
Ectonucleotidase
Myocardial infarction
Purigernic signaling
Regeneration
description The ectoenzymes CD39 and CD73 regulate the purinergic signaling through the hydrolysis of adenosine triphosphate (ATP)/ADP to AMP and to adenosine (Ado), respectively. This shifts the pro-inflammatory milieu induced by extracellular ATP to the anti- inflammatory regulation by Ado. Mesenchymal stem cells (MSCs) have potent immunomodulatory capabilities, including monocyte modulation toward an anti-inflammatory phenotype aiding tissue repair. In vitro, we observed that human cardiac adipose tissue-derived MSCs (cATMSCs) and umbilical cord MSCs similarly polarize monocytes toward a regulatory M2 phenotype, which maintained the expression of CD39 and induced expression of CD73 in a cell contact dependent fashion, correlating with increased functional activity. In addition, the local treatment with porcine cATMSCs using an engineered bioactive graft promoted the in vivo CD73 expression on host monocytes in a swine model of myocardial infarction. Our results suggest the upregulation of ectonucleotidases on MSC-conditioned monocytes as an effective mechanism to amplify the long-lasting immunomodulatory and healing effects of MSCs delivery.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/196945
https://dx.doi.org/urn:doi:10.3389/fimmu.2017.01577
url https://ddd.uab.cat/record/196945
https://dx.doi.org/urn:doi:10.3389/fimmu.2017.01577
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI14/01682
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-804
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-699
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 REDinREN/16/0009
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2014-59892-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00403
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 RD16/00111/0006
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014FIB00649
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418831387033600
score 15,300719

Similares en LA Referencia