When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225

Molluscs, like other invertebrates, have primitive defense systems. These are based on chemotaxis, recognition and facultative phagocytosis of foreign elements. Previously, we have described one of these systems: a cellular reaction involving infiltrated granulocytes against Perkinsus sp. parasitizi...

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Detalles Bibliográficos
Autores: Montes Castillo, Juan Francisco, Durfort i Coll, Mercè, Garcia Valero, Josep
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:1996
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183544
Acceso en línea:https://hdl.handle.net/2445/183544
Access Level:acceso abierto
Palabra clave:Parasitisme
Cloïsses
Parasitism
Clams
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spelling When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225Montes Castillo, Juan FranciscoDurfort i Coll, MercèGarcia Valero, JosepParasitismeCloïssesParasitismClamsMolluscs, like other invertebrates, have primitive defense systems. These are based on chemotaxis, recognition and facultative phagocytosis of foreign elements. Previously, we have described one of these systems: a cellular reaction involving infiltrated granulocytes against Perkinsus sp. parasitizing the Manila clam Tapes semidecussatus, in which the parasites are encapsulated by a defensive host product, the polypeptide p225. The aim of this study is to determine the similarities between the defense mechanisms of 2 venerid clams, T. semidecussatus and T. decussatus, when they are infected by Perkinsus sp. The hemocytes of both species infiltrate the connective tissue, redifferentiate, and ultimately, express and secrete the polypeptide which constitutes the main product of the capsule that surrounds the parasites. The main secretion product of T. decussatus shows a high degree of homology to that of T. semidecussatus, since it has a similar electrophoretic mobility and the polypeptide is recognized by the polyclonal serrum against p225 from T. semidecussatus, as confirmed by Western blotting and immunocytochemistry. In conclusion, we demonstrate the existence of 2 polypeptides that are closely related at the molecular and functional level, and are specific in the defense of some molluscs against infection by these protozoan parasites.Inter-Research2022202219962022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion9 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/183544Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.3354/dao026149Diseases of Aquatic Organisms, 1996, vol. 26, num. 2, p. 149-157https://doi.org/10.3354/dao026149(c) Inter-Research, 1996info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1835442026-05-29T05:05:01Z
dc.title.none.fl_str_mv When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
title When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
spellingShingle When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
Montes Castillo, Juan Francisco
Parasitisme
Cloïsses
Parasitism
Clams
title_short When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
title_full When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
title_fullStr When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
title_full_unstemmed When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
title_sort When the venerid clam Tapes decussatus is parasitized by the protozoan Perkinsus sp. it synthesizes a defensive polypeptide that is closely related to p225
dc.creator.none.fl_str_mv Montes Castillo, Juan Francisco
Durfort i Coll, Mercè
Garcia Valero, Josep
author Montes Castillo, Juan Francisco
author_facet Montes Castillo, Juan Francisco
Durfort i Coll, Mercè
Garcia Valero, Josep
author_role author
author2 Durfort i Coll, Mercè
Garcia Valero, Josep
author2_role author
author
dc.subject.none.fl_str_mv Parasitisme
Cloïsses
Parasitism
Clams
topic Parasitisme
Cloïsses
Parasitism
Clams
description Molluscs, like other invertebrates, have primitive defense systems. These are based on chemotaxis, recognition and facultative phagocytosis of foreign elements. Previously, we have described one of these systems: a cellular reaction involving infiltrated granulocytes against Perkinsus sp. parasitizing the Manila clam Tapes semidecussatus, in which the parasites are encapsulated by a defensive host product, the polypeptide p225. The aim of this study is to determine the similarities between the defense mechanisms of 2 venerid clams, T. semidecussatus and T. decussatus, when they are infected by Perkinsus sp. The hemocytes of both species infiltrate the connective tissue, redifferentiate, and ultimately, express and secrete the polypeptide which constitutes the main product of the capsule that surrounds the parasites. The main secretion product of T. decussatus shows a high degree of homology to that of T. semidecussatus, since it has a similar electrophoretic mobility and the polypeptide is recognized by the polyclonal serrum against p225 from T. semidecussatus, as confirmed by Western blotting and immunocytochemistry. In conclusion, we demonstrate the existence of 2 polypeptides that are closely related at the molecular and functional level, and are specific in the defense of some molluscs against infection by these protozoan parasites.
publishDate 1996
dc.date.none.fl_str_mv 1996
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183544
url https://hdl.handle.net/2445/183544
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.3354/dao026149
Diseases of Aquatic Organisms, 1996, vol. 26, num. 2, p. 149-157
https://doi.org/10.3354/dao026149
dc.rights.none.fl_str_mv (c) Inter-Research, 1996
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Inter-Research, 1996
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inter-Research
publisher.none.fl_str_mv Inter-Research
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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