An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer

The current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 microm deep pillars of 15.3 microm diameter with...

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Autores: Illa, Xavi, De Malsche, Wim, Bomer, Johan, Gardeniers, Han, Eijkel, Jan, Morante, Joan Ramon, Romano-Rodríguez, Albert, Desmet, Gert
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/377279
Acceso en línea:http://hdl.handle.net/10261/377279
https://api.elsevier.com/content/abstract/scopus_id/66149143037
Access Level:acceso abierto
Palabra clave:Cyclo olefin polymer
Diffraction
Fluorescence microscope
Liquid chromatography
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymerIlla, XaviDe Malsche, WimBomer, JohanGardeniers, HanEijkel, JanMorante, Joan RamonRomano-Rodríguez, AlbertDesmet, GertCyclo olefin polymerDiffractionFluorescence microscopeLiquid chromatographyhttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesThe current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 microm deep pillars of 15.3 microm diameter with an external porosity of 43 % and a well designed sidewall region to avoid side wall induced band broadening. A closed channel configuration was obtained by pressure assisted thermal bonding to a non-processed COP lid. Injection of coumarin dye plugs and detection with a fluorescence microscope showed very close agreement of this channel configuration to theoretical expectations in terms of band broadening. This agreement is due to the low taper, the optimized sidewall region and the excellent bonding quality between the two polymer sheets, even at the pillar area. Under non-retained conditions (pure methanol as mobile phase), plate heights as low as 4 microm were obtained. Under retained conditions, using the native hydrophobic properties of the COP channel (in 70/30 v/v water/methanol mixture as mobile phase), a minimum plate height of 6 microm was obtained. A 4 component separation was successfully achieved, demonstrating that COP is a cheap and efficient alternative for silicon and silica based liquid chromatography formats.This work has been partially supported by the Spanish Ministry of Education and Science through the projects CROMINA (ref. TEC2004-06854-C03-01/MIC) and ISIS (ref. TEC2007-67962-C04-04/MIC) and through the FPI grant of one of the authors (X.I.). The autors appreciate access and use of the Nanolithography Laboratory at CNM-IMB and the Nanotechnology Platform at PCB-UB where part of the chip fabrication was also done.NoRoyal Society of Chemistry (UK)Ministerio de Educación y Ciencia (España)202520252009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/377279https://api.elsevier.com/content/abstract/scopus_id/66149143037reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MIC/TEC2004-06854-C03-01/info:eu-repo/grantAgreement/MIC/TEC2007-67962-C04-04/Lab on a chiphttps://doi.org/10.1039/B818918HNoinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3772792026-05-22T06:33:51Z
dc.title.none.fl_str_mv An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
title An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
spellingShingle An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
Illa, Xavi
Cyclo olefin polymer
Diffraction
Fluorescence microscope
Liquid chromatography
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
title_full An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
title_fullStr An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
title_full_unstemmed An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
title_sort An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer
dc.creator.none.fl_str_mv Illa, Xavi
De Malsche, Wim
Bomer, Johan
Gardeniers, Han
Eijkel, Jan
Morante, Joan Ramon
Romano-Rodríguez, Albert
Desmet, Gert
author Illa, Xavi
author_facet Illa, Xavi
De Malsche, Wim
Bomer, Johan
Gardeniers, Han
Eijkel, Jan
Morante, Joan Ramon
Romano-Rodríguez, Albert
Desmet, Gert
author_role author
author2 De Malsche, Wim
Bomer, Johan
Gardeniers, Han
Eijkel, Jan
Morante, Joan Ramon
Romano-Rodríguez, Albert
Desmet, Gert
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
dc.subject.none.fl_str_mv Cyclo olefin polymer
Diffraction
Fluorescence microscope
Liquid chromatography
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic Cyclo olefin polymer
Diffraction
Fluorescence microscope
Liquid chromatography
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description The current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 microm deep pillars of 15.3 microm diameter with an external porosity of 43 % and a well designed sidewall region to avoid side wall induced band broadening. A closed channel configuration was obtained by pressure assisted thermal bonding to a non-processed COP lid. Injection of coumarin dye plugs and detection with a fluorescence microscope showed very close agreement of this channel configuration to theoretical expectations in terms of band broadening. This agreement is due to the low taper, the optimized sidewall region and the excellent bonding quality between the two polymer sheets, even at the pillar area. Under non-retained conditions (pure methanol as mobile phase), plate heights as low as 4 microm were obtained. Under retained conditions, using the native hydrophobic properties of the COP channel (in 70/30 v/v water/methanol mixture as mobile phase), a minimum plate height of 6 microm was obtained. A 4 component separation was successfully achieved, demonstrating that COP is a cheap and efficient alternative for silicon and silica based liquid chromatography formats.
publishDate 2009
dc.date.none.fl_str_mv 2009
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/377279
https://api.elsevier.com/content/abstract/scopus_id/66149143037
url http://hdl.handle.net/10261/377279
https://api.elsevier.com/content/abstract/scopus_id/66149143037
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MIC/TEC2004-06854-C03-01/
info:eu-repo/grantAgreement/MIC/TEC2007-67962-C04-04/
Lab on a chip
https://doi.org/10.1039/B818918H
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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