Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity

Carps, barbels and other cyprinids are the major contributors to freshwater aquaculture at global scale. Nevertheless, freshwater fish aquaculture needs to diversify their production in order to offer consumers new species. Tench (Tinca tinca) is a freshwater species with great interest for the dive...

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Autores: Garrido, Diego, Monroig, Óscar, Galindo, A., Betancor, Mónica B., Pérez, José A., Kabeya, Naoki, Marrero, M., Rodríguez, Covadonga
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202227
Acceso en línea:http://hdl.handle.net/10261/202227
Access Level:acceso abierto
Palabra clave:Biosynthesis
Fatty acyl desaturases
Fatty acyl elongases
Long-chain polyunsaturated fatty acids
Radiolabelled fatty acid
Tinca tinca
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spelling Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacityGarrido, DiegoMonroig, ÓscarGalindo, A.Betancor, Mónica B.Pérez, José A.Kabeya, NaokiMarrero, M.Rodríguez, CovadongaBiosynthesisFatty acyl desaturasesFatty acyl elongasesLong-chain polyunsaturated fatty acidsRadiolabelled fatty acidTinca tincaCarps, barbels and other cyprinids are the major contributors to freshwater aquaculture at global scale. Nevertheless, freshwater fish aquaculture needs to diversify their production in order to offer consumers new species. Tench (Tinca tinca) is a freshwater species with great interest for the diversification of continental aquaculture. However, up to date, no commercial formulated diet exists for this species in order to optimize their nutritional requirements and the quality of its final product. Using multiple methodological approaches, the aim of this study was to evaluate the long chain polyunsaturated fatty acid (LC-PUFA) metabolism of T. tinca. Firstly, the molecular cloning and functional characterisation by heterologous expression in yeast of a desaturase (Fads2) and two elongases (Elovl2 and Elovl5) involved in LC-PUFA biosynthesis, and the analysis of gene expression among tissues were performed. Secondly, in order to confirm the LC-PUFA biosynthesis capacity of isolated hepatocytes and enterocytes, cells were incubated with [1-14C] labelled linoleic acid (18:2n-6, LA), linolenic acid (18:3n-3, ALA) and eicosapentaenoic acid (20:5n-3, EPA). In yeast, Fads2 showed a Δ6/Δ5 bifunctional activity. Elovl2 was more active over C20 and C22 substrates, whereas Elovl5 was over C18 and C20. Liver displayed the highest expression for the three target genes (fads2, elovl2 and elovl5). Incubated cells also showed Fads2 bifunctional activity as well as elongation products in concordance with yeast heterologous expression results. Importantly, our results demonstrated that tench is able to biosynthesise docosahexaenoic acid (DHA) from 18:3n-3 in both hepatocytes and enterocytes, a capacity that seems to explain in part the surprisingly high levels of DHA found in the fish flesh compared to its dietary supply. Tench is a promising freshwater species with a potential capacity to endogenously increase its flesh DHA contents, reducing the impact that the usage of fish oils from forage fisheries may have on the aquaculture industry.This study was funded by Ministerio de Economía y Competitividad (AGL2015-70994-R).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Monroig, Óscar [0000-0001-8712-0440]Kabeya, Naoki [0000-0002-2055-6554]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/202227reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70994-Rhttp://dx.doi.org/10.1016/j.aquaculture.2020.735147Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2022272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
title Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
spellingShingle Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
Garrido, Diego
Biosynthesis
Fatty acyl desaturases
Fatty acyl elongases
Long-chain polyunsaturated fatty acids
Radiolabelled fatty acid
Tinca tinca
title_short Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
title_full Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
title_fullStr Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
title_full_unstemmed Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
title_sort Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity
dc.creator.none.fl_str_mv Garrido, Diego
Monroig, Óscar
Galindo, A.
Betancor, Mónica B.
Pérez, José A.
Kabeya, Naoki
Marrero, M.
Rodríguez, Covadonga
author Garrido, Diego
author_facet Garrido, Diego
Monroig, Óscar
Galindo, A.
Betancor, Mónica B.
Pérez, José A.
Kabeya, Naoki
Marrero, M.
Rodríguez, Covadonga
author_role author
author2 Monroig, Óscar
Galindo, A.
Betancor, Mónica B.
Pérez, José A.
Kabeya, Naoki
Marrero, M.
Rodríguez, Covadonga
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Monroig, Óscar [0000-0001-8712-0440]
Kabeya, Naoki [0000-0002-2055-6554]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biosynthesis
Fatty acyl desaturases
Fatty acyl elongases
Long-chain polyunsaturated fatty acids
Radiolabelled fatty acid
Tinca tinca
topic Biosynthesis
Fatty acyl desaturases
Fatty acyl elongases
Long-chain polyunsaturated fatty acids
Radiolabelled fatty acid
Tinca tinca
description Carps, barbels and other cyprinids are the major contributors to freshwater aquaculture at global scale. Nevertheless, freshwater fish aquaculture needs to diversify their production in order to offer consumers new species. Tench (Tinca tinca) is a freshwater species with great interest for the diversification of continental aquaculture. However, up to date, no commercial formulated diet exists for this species in order to optimize their nutritional requirements and the quality of its final product. Using multiple methodological approaches, the aim of this study was to evaluate the long chain polyunsaturated fatty acid (LC-PUFA) metabolism of T. tinca. Firstly, the molecular cloning and functional characterisation by heterologous expression in yeast of a desaturase (Fads2) and two elongases (Elovl2 and Elovl5) involved in LC-PUFA biosynthesis, and the analysis of gene expression among tissues were performed. Secondly, in order to confirm the LC-PUFA biosynthesis capacity of isolated hepatocytes and enterocytes, cells were incubated with [1-14C] labelled linoleic acid (18:2n-6, LA), linolenic acid (18:3n-3, ALA) and eicosapentaenoic acid (20:5n-3, EPA). In yeast, Fads2 showed a Δ6/Δ5 bifunctional activity. Elovl2 was more active over C20 and C22 substrates, whereas Elovl5 was over C18 and C20. Liver displayed the highest expression for the three target genes (fads2, elovl2 and elovl5). Incubated cells also showed Fads2 bifunctional activity as well as elongation products in concordance with yeast heterologous expression results. Importantly, our results demonstrated that tench is able to biosynthesise docosahexaenoic acid (DHA) from 18:3n-3 in both hepatocytes and enterocytes, a capacity that seems to explain in part the surprisingly high levels of DHA found in the fish flesh compared to its dietary supply. Tench is a promising freshwater species with a potential capacity to endogenously increase its flesh DHA contents, reducing the impact that the usage of fish oils from forage fisheries may have on the aquaculture industry.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202227
url http://hdl.handle.net/10261/202227
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70994-R
http://dx.doi.org/10.1016/j.aquaculture.2020.735147
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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