Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid
Antioxidant-based treatments have emerged as novel and interesting approaches to counteract fat accumulation in obesity and associated metabolic disturbances. Adipocytes from rats that were fed on chow or high-fat diet (HFD) for 50 d were isolated (primary adipocytes) and incubated (72 h) on low (LG...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/23063 |
| Acceso en línea: | https://hdl.handle.net/10171/23063 |
| Access Level: | acceso abierto |
| Palabra clave: | Vitamin C Diet-induced obesity Adipose tissue Cell culture Adipokines |
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Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acidGarcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817cArellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31bMilagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebMoreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Vitamin CDiet-induced obesityAdipose tissueCell cultureAdipokinesAntioxidant-based treatments have emerged as novel and interesting approaches to counteract fat accumulation in obesity and associated metabolic disturbances. Adipocytes from rats that were fed on chow or high-fat diet (HFD) for 50 d were isolated (primary adipocytes) and incubated (72 h) on low (LG; 5.6 mmol/L) or high (HG; 25 mmol/L) glucose levels, in the presence or absence of 1.6 nmol/L insulin and 200 μmol/L vitamin C (VC). Adipocytes from HFD-fed animals presented lower insulin-induced glucose uptake, lower lactate and glycerol release, and lower insulin-induced secretion of some adipokines as compared with controls. HG treatment restored the blunted response to insulin regarding apelin secretion in adipocytes from HFD-fed rats. VC treatment inhibited the levels of nearly all variables, irrespective of the adipocytes' dietary origin. The HG treatment reduced adipocyte viability, and VC protected from this toxic effect, although more drastically in control adipocytes. Summing up, in vivo chow or HFD intake determines a differential response to insulin and glucose treatments that appears to be dependent on the insulin-resistance status of the adipocytes, while VC modifies some responses from adipocytes independently of the previous dietary intake of the animals.Royal Society of Medicine PressDadun. Depósito Académico Digital Universidad de Navarra20122012-08-2220122012-01-0120122012-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/23063reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/230632026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| title |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| spellingShingle |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3 Vitamin C Diet-induced obesity Adipose tissue Cell culture Adipokines |
| title_short |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| title_full |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| title_fullStr |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| title_full_unstemmed |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| title_sort |
Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid |
| dc.creator.none.fl_str_mv |
Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3 Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82 Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 |
| author |
Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3 |
| author_facet |
Garcia-Diaz, D.F. (Diego F.)|||/items/920c7c68-4cd2-42ba-970a-e2592a210df3 Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82 Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 |
| author_role |
author |
| author2 |
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c Arellano, A.V. (A.V.)|||/items/a1578bd4-69bb-4670-987a-eff5b1b6a31b Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82 Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131 |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Vitamin C Diet-induced obesity Adipose tissue Cell culture Adipokines |
| topic |
Vitamin C Diet-induced obesity Adipose tissue Cell culture Adipokines |
| description |
Antioxidant-based treatments have emerged as novel and interesting approaches to counteract fat accumulation in obesity and associated metabolic disturbances. Adipocytes from rats that were fed on chow or high-fat diet (HFD) for 50 d were isolated (primary adipocytes) and incubated (72 h) on low (LG; 5.6 mmol/L) or high (HG; 25 mmol/L) glucose levels, in the presence or absence of 1.6 nmol/L insulin and 200 μmol/L vitamin C (VC). Adipocytes from HFD-fed animals presented lower insulin-induced glucose uptake, lower lactate and glycerol release, and lower insulin-induced secretion of some adipokines as compared with controls. HG treatment restored the blunted response to insulin regarding apelin secretion in adipocytes from HFD-fed rats. VC treatment inhibited the levels of nearly all variables, irrespective of the adipocytes' dietary origin. The HG treatment reduced adipocyte viability, and VC protected from this toxic effect, although more drastically in control adipocytes. Summing up, in vivo chow or HFD intake determines a differential response to insulin and glucose treatments that appears to be dependent on the insulin-resistance status of the adipocytes, while VC modifies some responses from adipocytes independently of the previous dietary intake of the animals. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 2012-08-22 2012 2012-01-01 2012 2012-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/23063 |
| url |
https://hdl.handle.net/10171/23063 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Royal Society of Medicine Press |
| publisher.none.fl_str_mv |
Royal Society of Medicine Press |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15.300719 |