2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium

Structure-function relationships of a novel 20-deoxyribosyltransferase from the psychrotolerant bacterium Bacillus psychrosaccharolyticus (BpNDT) have been exhaustively studied by biochemical and high resolution crystallographic analyses. Despite BpNDT exhibiting some structural features characteris...

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Detalles Bibliográficos
Autores: Fresco Taboada, Alba, Fernández Lucas, Jesús, Acebal Sarabia, Carmen, Arroyo Sánchez, Miguel, Ramón, Fernando, De La Mata Riesco, Mª Isabel, Mancheño, José
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/12486
Acceso en línea:https://hdl.handle.net/20.500.14352/12486
Access Level:acceso abierto
Palabra clave:577.15
20-deoxyribosyltransferase
enzymatic synthesis
oligomeric assembly
protein crystallography
nucleoside analogues
therapeutic nucleosides
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/12486
network_acronym_str ES
network_name_str España
repository_id_str
spelling 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant BacteriumFresco Taboada, AlbaFernández Lucas, JesúsAcebal Sarabia, CarmenArroyo Sánchez, MiguelRamón, FernandoDe La Mata Riesco, Mª IsabelMancheño, José577.1520-deoxyribosyltransferaseenzymatic synthesisoligomeric assemblyprotein crystallographynucleoside analoguestherapeutic nucleosidesBiología molecular (Biología)Bioquímica (Biología)2415 Biología Molecular2302 BioquímicaStructure-function relationships of a novel 20-deoxyribosyltransferase from the psychrotolerant bacterium Bacillus psychrosaccharolyticus (BpNDT) have been exhaustively studied by biochemical and high resolution crystallographic analyses. Despite BpNDT exhibiting some structural features characteristic of cold-adapted enzymes such as localized flexibility in critical loops, its biochemical properties are typical of mesophilic enzymes. BpNDT is a highly symmetrical homohexamer with tightly associated subunits that possesses flexible and short loops bordering the active sites. The catalytic center is essentially identical to that of other mesophilic homologues. Moreover, BpNDT shows that it is a mesophilic-like enzyme since it is not heat-labile and exhibits an apparent unfolding temperature (Tm) of 49 ◦C, being active during 96 h at 40 and 50 ◦C. Finally, BpNDT synthesizes natural and modified nucleosides, with preference for purines as acceptors and pyrimidine nucleosides as donors. Remarkably, the synthesis of several therapeutic nucleosides has been efficiently carried out. In this sense, 5-hydroxymethyl-20 -deoxyuridine (5-HMdUrd), 7-deaza-6-hydroxypurine-20 -deoxyriboside (7-DHPdRib) and theophylline-20 -deoxyriboside were synthesized for the first time by an NDT enzyme, showing the biotechnological interest of BpNDT.MDPIUniversidad Complutense de Madrid20182018-01-0320182018-01-03journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12486reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/124862026-06-02T12:44:21Z
dc.title.none.fl_str_mv 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
title 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
spellingShingle 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
Fresco Taboada, Alba
577.15
20-deoxyribosyltransferase
enzymatic synthesis
oligomeric assembly
protein crystallography
nucleoside analogues
therapeutic nucleosides
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
title_short 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
title_full 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
title_fullStr 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
title_full_unstemmed 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
title_sort 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
dc.creator.none.fl_str_mv Fresco Taboada, Alba
Fernández Lucas, Jesús
Acebal Sarabia, Carmen
Arroyo Sánchez, Miguel
Ramón, Fernando
De La Mata Riesco, Mª Isabel
Mancheño, José
author Fresco Taboada, Alba
author_facet Fresco Taboada, Alba
Fernández Lucas, Jesús
Acebal Sarabia, Carmen
Arroyo Sánchez, Miguel
Ramón, Fernando
De La Mata Riesco, Mª Isabel
Mancheño, José
author_role author
author2 Fernández Lucas, Jesús
Acebal Sarabia, Carmen
Arroyo Sánchez, Miguel
Ramón, Fernando
De La Mata Riesco, Mª Isabel
Mancheño, José
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.15
20-deoxyribosyltransferase
enzymatic synthesis
oligomeric assembly
protein crystallography
nucleoside analogues
therapeutic nucleosides
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
topic 577.15
20-deoxyribosyltransferase
enzymatic synthesis
oligomeric assembly
protein crystallography
nucleoside analogues
therapeutic nucleosides
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
description Structure-function relationships of a novel 20-deoxyribosyltransferase from the psychrotolerant bacterium Bacillus psychrosaccharolyticus (BpNDT) have been exhaustively studied by biochemical and high resolution crystallographic analyses. Despite BpNDT exhibiting some structural features characteristic of cold-adapted enzymes such as localized flexibility in critical loops, its biochemical properties are typical of mesophilic enzymes. BpNDT is a highly symmetrical homohexamer with tightly associated subunits that possesses flexible and short loops bordering the active sites. The catalytic center is essentially identical to that of other mesophilic homologues. Moreover, BpNDT shows that it is a mesophilic-like enzyme since it is not heat-labile and exhibits an apparent unfolding temperature (Tm) of 49 ◦C, being active during 96 h at 40 and 50 ◦C. Finally, BpNDT synthesizes natural and modified nucleosides, with preference for purines as acceptors and pyrimidine nucleosides as donors. Remarkably, the synthesis of several therapeutic nucleosides has been efficiently carried out. In this sense, 5-hydroxymethyl-20 -deoxyuridine (5-HMdUrd), 7-deaza-6-hydroxypurine-20 -deoxyriboside (7-DHPdRib) and theophylline-20 -deoxyriboside were synthesized for the first time by an NDT enzyme, showing the biotechnological interest of BpNDT.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-03
2018
2018-01-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12486
url https://hdl.handle.net/20.500.14352/12486
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418792723939328
score 15,301603

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