Polymeric nanocapsules for the ocular delivery of siRNA
The development of siRNA-based therapies for the treatment of severe ocular diseases is being proposed as a promising therapeutic option for the treatment of severe ocular diseases. Unfortunately, the capacity of siRNA molecules to overcome the multiple protective barriers of the eye is very limited...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universidad de Santiago de Compostela (USC) |
| Repositorio: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Idioma: | inglés |
| OAI Identifier: | oai:minerva.usc.gal:10347/20532 |
| Acceso en línea: | http://hdl.handle.net/10347/20532 |
| Access Level: | acceso abierto |
| Palabra clave: | Materias::Investigación::32 Ciencias médicas::3209 Farmacología::320908 Preparación de medicamentos Materias::Investigación::32 Ciencias médicas::3209 Farmacología::320990 Farmacología experimental Materias::Investigación::23 Química::2302 Bioquímica::230223 Ácidos nucleicos |
| Sumario: | The development of siRNA-based therapies for the treatment of severe ocular diseases is being proposed as a promising therapeutic option for the treatment of severe ocular diseases. Unfortunately, the capacity of siRNA molecules to overcome the multiple protective barriers of the eye is very limited. The specific objective of this work was to develop multiplayer protamine NCs loading high amounts of siRNA (up to 5 mg/mL) for ocular topical administration. Different preparation methods and compositions were studied for the development of NCs complying with the requirements of drug loading and release and also in terms of stability. Among the multiple formulations developed, one of them led to acceptable silencing results in vitro and showed the ability to interact efficiently with the rabbits’ corneal epithelium. |
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