Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response

Methyl jasmonate (MeJa) is a plant chemical elicitor which has been used to artificially induce chemical defensive responses and resistance against herbivores in many plant species in recent decades. In this paper, we studied the effect of exogenous MeJa application at different concentrations (0, 5...

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Autores: Moreira Tomé, Xoaquín, Sampedro Pérez, Luis, Zas Arregui, Rafael
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24099
Acceso en línea:http://hdl.handle.net/10261/24099
Access Level:acceso abierto
Palabra clave:Constitutive defences
Induced defense
Large pine weevil
Resin content
Phenolic compounds
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spelling Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic responseMoreira Tomé, XoaquínSampedro Pérez, LuisZas Arregui, RafaelConstitutive defencesInduced defenseLarge pine weevilResin contentPhenolic compoundsMethyl jasmonate (MeJa) is a plant chemical elicitor which has been used to artificially induce chemical defensive responses and resistance against herbivores in many plant species in recent decades. In this paper, we studied the effect of exogenous MeJa application at different concentrations (0, 5, 50, and 100 mM) on growth, chemical defenses and resistance against the large pine weevil Hylobius abietis L in one year old Pinus pinaster Ait. seedlings. We also investigated whether the local application of MeJa on a basal branch would distally elicit defensive responses or growth reductions on the opposite branch and on the upper main stem. Exogenous application of MeJa induced resin accumulation in the stems, and enhanced resistance against H. abietis. The resin content in plants treated with 100 mM was nearly two-fold greater, and the pine weevil consumed 80% less phloem than in control plants. However, concentration of total phenolics in needles was lower in plants treated with the higher MeJa dose, probably because of a local toxic effect. We did not detect any effect of MeJa application on plant growth traits. In addition, the application of MeJa on the experimental branches did not significantly affect the resin content, nor growth rates of the opposite untreated control branches. However, the application of MeJa on the experimental branches significantly increased the resin content in the stem and total phenolics in the needles in the apical stem section of the seedling. These results suggest that the systemic effect of the MeJa induction is restricted to the most valuable parts of the seedlings, and should be considered in further studies aimed to understand the systemic resistance of conifers.Research was supported by the INIA RTA05-173 and INIA RTA07-100 projects.Peer reviewedElsevier201020102009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501185394 bytesapplication/pdfhttp://hdl.handle.net/10261/24099reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.envexpbot.2009.05.015info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/240992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
title Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
spellingShingle Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
Moreira Tomé, Xoaquín
Constitutive defences
Induced defense
Large pine weevil
Resin content
Phenolic compounds
title_short Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
title_full Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
title_fullStr Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
title_full_unstemmed Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
title_sort Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response
dc.creator.none.fl_str_mv Moreira Tomé, Xoaquín
Sampedro Pérez, Luis
Zas Arregui, Rafael
author Moreira Tomé, Xoaquín
author_facet Moreira Tomé, Xoaquín
Sampedro Pérez, Luis
Zas Arregui, Rafael
author_role author
author2 Sampedro Pérez, Luis
Zas Arregui, Rafael
author2_role author
author
dc.subject.none.fl_str_mv Constitutive defences
Induced defense
Large pine weevil
Resin content
Phenolic compounds
topic Constitutive defences
Induced defense
Large pine weevil
Resin content
Phenolic compounds
description Methyl jasmonate (MeJa) is a plant chemical elicitor which has been used to artificially induce chemical defensive responses and resistance against herbivores in many plant species in recent decades. In this paper, we studied the effect of exogenous MeJa application at different concentrations (0, 5, 50, and 100 mM) on growth, chemical defenses and resistance against the large pine weevil Hylobius abietis L in one year old Pinus pinaster Ait. seedlings. We also investigated whether the local application of MeJa on a basal branch would distally elicit defensive responses or growth reductions on the opposite branch and on the upper main stem. Exogenous application of MeJa induced resin accumulation in the stems, and enhanced resistance against H. abietis. The resin content in plants treated with 100 mM was nearly two-fold greater, and the pine weevil consumed 80% less phloem than in control plants. However, concentration of total phenolics in needles was lower in plants treated with the higher MeJa dose, probably because of a local toxic effect. We did not detect any effect of MeJa application on plant growth traits. In addition, the application of MeJa on the experimental branches did not significantly affect the resin content, nor growth rates of the opposite untreated control branches. However, the application of MeJa on the experimental branches significantly increased the resin content in the stem and total phenolics in the needles in the apical stem section of the seedling. These results suggest that the systemic effect of the MeJa induction is restricted to the most valuable parts of the seedlings, and should be considered in further studies aimed to understand the systemic resistance of conifers.
publishDate 2009
dc.date.none.fl_str_mv 2009
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24099
url http://hdl.handle.net/10261/24099
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.envexpbot.2009.05.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 185394 bytes
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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