Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20

During conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance. Conjugative elements can contain exclusion system(s) that inhibit its transfer to a cell already harbori...

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Autores: Gago-Córdoba, César, Val-Calvo, Jorge, Miguel-Arribas, Andrés, Serrano, Ester, Singh, Praveen K., Abia, David, Wu, Ling Juan, Meijer, Wilfried J. J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/216151
Acceso en línea:http://hdl.handle.net/10261/216151
Access Level:acceso abierto
Palabra clave:Surface exclusion
Antibiotic resistance
Firmicutes
Horizontal gene transfer
Conjugation
Grampositive bacteria
Gene expression
Surface protein
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spelling Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20Gago-Córdoba, CésarVal-Calvo, JorgeMiguel-Arribas, AndrésSerrano, EsterSingh, Praveen K.Abia, DavidWu, Ling JuanMeijer, Wilfried J. J.Surface exclusionAntibiotic resistanceFirmicutesHorizontal gene transferConjugationGrampositive bacteriaGene expressionSurface proteinDuring conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance. Conjugative elements can contain exclusion system(s) that inhibit its transfer to a cell already harboring the element. Our limited knowledge on exclusion systems is mainly based on plasmids of Gram-negative bacteria. Here we studied the conjugative plasmid pLS20 of the Gram-positive Bacillus subtilis. We demonstrate that pLS20 contains an exclusion system and identified the single gene responsible for exclusion, named sespLS20, which is embedded in the conjugation operon. SespLS20 is the founding member of a novel family of surface exclusion proteins encoded by conjugative elements of Gram-positive origin. We show that the extent of surface exclusion correlates with the level of sespLS20 expression, and that sespLS20 is expressed at basal low-levels in all donor cells but becomes highly expressed in conjugating cells. Accordingly, the transfer of pLS20 from a conjugation-primed donor cell to an un-primed or conjugation-primed donor is inhibited moderately and very efficiently, respectively. The consequences of this differential regulation, which appears to be a conserved feature of surface exclusion systems of Gram-positive and Gram-negative origin, are discussed.Ministry of Economy and Competitiveness; “Agencia Estatal de Investigación (AEI)” and “Fondo Europeo de Desarrollo Regional (FEDER).” This research was also supported by institutional grants from the “Fundación Ramón Areces” and “Banco de Santander” to the Centro de Biología Molecular “Severo OchoaMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Fundación Ramón ArecesBanco SantanderConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/216151reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fmicb.2019.01502Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2161512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
title Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
spellingShingle Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
Gago-Córdoba, César
Surface exclusion
Antibiotic resistance
Firmicutes
Horizontal gene transfer
Conjugation
Grampositive bacteria
Gene expression
Surface protein
title_short Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
title_full Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
title_fullStr Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
title_full_unstemmed Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
title_sort Surface exclusion revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
dc.creator.none.fl_str_mv Gago-Córdoba, César
Val-Calvo, Jorge
Miguel-Arribas, Andrés
Serrano, Ester
Singh, Praveen K.
Abia, David
Wu, Ling Juan
Meijer, Wilfried J. J.
author Gago-Córdoba, César
author_facet Gago-Córdoba, César
Val-Calvo, Jorge
Miguel-Arribas, Andrés
Serrano, Ester
Singh, Praveen K.
Abia, David
Wu, Ling Juan
Meijer, Wilfried J. J.
author_role author
author2 Val-Calvo, Jorge
Miguel-Arribas, Andrés
Serrano, Ester
Singh, Praveen K.
Abia, David
Wu, Ling Juan
Meijer, Wilfried J. J.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Fundación Ramón Areces
Banco Santander
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Surface exclusion
Antibiotic resistance
Firmicutes
Horizontal gene transfer
Conjugation
Grampositive bacteria
Gene expression
Surface protein
topic Surface exclusion
Antibiotic resistance
Firmicutes
Horizontal gene transfer
Conjugation
Grampositive bacteria
Gene expression
Surface protein
description During conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance. Conjugative elements can contain exclusion system(s) that inhibit its transfer to a cell already harboring the element. Our limited knowledge on exclusion systems is mainly based on plasmids of Gram-negative bacteria. Here we studied the conjugative plasmid pLS20 of the Gram-positive Bacillus subtilis. We demonstrate that pLS20 contains an exclusion system and identified the single gene responsible for exclusion, named sespLS20, which is embedded in the conjugation operon. SespLS20 is the founding member of a novel family of surface exclusion proteins encoded by conjugative elements of Gram-positive origin. We show that the extent of surface exclusion correlates with the level of sespLS20 expression, and that sespLS20 is expressed at basal low-levels in all donor cells but becomes highly expressed in conjugating cells. Accordingly, the transfer of pLS20 from a conjugation-primed donor cell to an un-primed or conjugation-primed donor is inhibited moderately and very efficiently, respectively. The consequences of this differential regulation, which appears to be a conserved feature of surface exclusion systems of Gram-positive and Gram-negative origin, are discussed.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/216151
url http://hdl.handle.net/10261/216151
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3389/fmicb.2019.01502
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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