Hypoxia-inducible factor-1 modulates the expression of vascular endothelial growth factor and endothelial nitric oxide synthase induced by eccentric exercise
[EN] The present study investigated the effects of acute and chronic eccentric exercise on the hypoxia-inducible factor (HIF)-1 activation response and the con- comitant modulation of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) expression in rat skeletal mu...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Recursos: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:dnet:buleria_____::5de917061b65a5e5832ac6ec8d33fc03 |
| Acesso em linha: | https://journals.physiology.org/doi/full/10.1152/japplphysiol.00780.2014 https://hdl.handle.net/10612/28240 |
| Access Level: | acceso abierto |
| Palavra-chave: | Educación Física Fisiología Veterinaria Eccentric exercise Hypoxia-inducible factor-1 Vascular endothelial growth factor Endothelial nitric oxide synthase 3109 Ciencias Veterinarias 2411.10 Fisiología del Músculo |
| Resumo: | [EN] The present study investigated the effects of acute and chronic eccentric exercise on the hypoxia-inducible factor (HIF)-1 activation response and the con- comitant modulation of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) expression in rat skeletal muscle. Twenty-four male Wistar rats were randomly assigned to three experimental groups: rested control group, acutely exercised group after an intermittent downhill protocol for 90 min, and acutely exercise group with a previous eccentric training of 8 wk. HIF-1 activation, VEGF and eNOS gene expression, protein content, and promoter activation were assessed in vastus lateralis muscle biopsies. Acute eccentric exercise induced a marked activation of HIF-1 and resulted in increased VEGF and eNOS mRNA level and protein concentration. The binding of HIF-1 to the VEGF and eNOS promoters, measured by a chromatin immunoprecipitation assay, was undetectable in rested rats, whereas it was evident in acutely exercised animals. Acute exercise also increased myeloperoxidase, toll-like receptor-4, tumor necrosis factor- , and interleukin-1 protein con- tent, suggesting a contribution of proinflammatory stimuli to HIF-1 activation and VEGF overexpression. All of these effects were par- tially abolished by training. Moreover, training resulted in an in- creased capillary density. In summary, our findings indicate that eccentric exercise prompts an HIF-1 response in untrained skeletal muscle that contributes to the upregulation of VEGF and eNOS gene expression and is attenuated after an eccentric training program |
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