Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line

Objective : Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S.aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S.aureus interaction within a m...

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Autores: Lacoma, Alicia|||0000-0002-2049-3872, Cano, Victoria|||0000-0002-2537-4063, Moranta, D.|||0000-0001-9528-4920, Regueiro, Verónica, Domínguez Villanueva, Dídac, Laabei, Maisem|||0000-0002-8425-3704, González-Nicolau, M., Ausina, Vicente|||0000-0002-1798-9869, Prat, C., Bengoechea, J. A.
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:187835
Acceso en línea:https://ddd.uab.cat/record/187835
https://dx.doi.org/urn:doi:10.1080/21505594.2017.1361089
Access Level:acceso abierto
Palabra clave:Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
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spelling Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell lineLacoma, Alicia|||0000-0002-2049-3872Cano, Victoria|||0000-0002-2537-4063Moranta, D.|||0000-0001-9528-4920Regueiro, VerónicaDomínguez Villanueva, DídacLaabei, Maisem|||0000-0002-8425-3704González-Nicolau, M.Ausina, Vicente|||0000-0002-1798-9869Prat, C.Bengoechea, J. A.InvasionMacrophagePersistencePhagocytosisStaphylococcus aureusObjective : Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S.aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S.aureus interaction within a murine alveolar macrophage cell line. Methods : Cell line MH-S was infected with Newman strain. Molecular mechanisms involved in phagocytosis were explored. To assess whether S.aureus survives intracellularly quantitative (gentamicin protection assays and bacterial plating) and qualitative analysis (immunofluorescence microscopy) were performed. Bacterial colocalization with different markers of the endocytic pathway was examined to characterize its intracellular trafficking. Results : We found that S.aureus uptake requires host actin polymerization, microtubule assembly and activation of phosphatidylinositol 3-kinase signaling. Time course experiments showed that Newman strain was able to persist within macrophages at least until 28.5 h post infection. We observed that intracellular bacteria are located inside an acidic subcellular compartment, which co-localizes with the late endosome/lysosome markers Lamp-1, Rab7 and RILP. Colocalization counts with TMR-dextran might reflect a balance between bacterial killing and intracellular survival. Conclusions : This study indicates that S.aureus persists and replicates inside murine alveolar macrophages, representing a privileged niche that can potentially offer protection from antimicrobial activity and immunological host defense mechanisms.Universitat Autònoma de Barcelona 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/187835https://dx.doi.org/urn:doi:10.1080/21505594.2017.1361089reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI13-01418Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 SEPAR054-2011open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1878352026-06-06T12:50:31Z
dc.title.none.fl_str_mv Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
spellingShingle Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
Lacoma, Alicia|||0000-0002-2049-3872
Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
title_short Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_full Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_fullStr Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_full_unstemmed Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_sort Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
dc.creator.none.fl_str_mv Lacoma, Alicia|||0000-0002-2049-3872
Cano, Victoria|||0000-0002-2537-4063
Moranta, D.|||0000-0001-9528-4920
Regueiro, Verónica
Domínguez Villanueva, Dídac
Laabei, Maisem|||0000-0002-8425-3704
González-Nicolau, M.
Ausina, Vicente|||0000-0002-1798-9869
Prat, C.
Bengoechea, J. A.
author Lacoma, Alicia|||0000-0002-2049-3872
author_facet Lacoma, Alicia|||0000-0002-2049-3872
Cano, Victoria|||0000-0002-2537-4063
Moranta, D.|||0000-0001-9528-4920
Regueiro, Verónica
Domínguez Villanueva, Dídac
Laabei, Maisem|||0000-0002-8425-3704
González-Nicolau, M.
Ausina, Vicente|||0000-0002-1798-9869
Prat, C.
Bengoechea, J. A.
author_role author
author2 Cano, Victoria|||0000-0002-2537-4063
Moranta, D.|||0000-0001-9528-4920
Regueiro, Verónica
Domínguez Villanueva, Dídac
Laabei, Maisem|||0000-0002-8425-3704
González-Nicolau, M.
Ausina, Vicente|||0000-0002-1798-9869
Prat, C.
Bengoechea, J. A.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
topic Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
description Objective : Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S.aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S.aureus interaction within a murine alveolar macrophage cell line. Methods : Cell line MH-S was infected with Newman strain. Molecular mechanisms involved in phagocytosis were explored. To assess whether S.aureus survives intracellularly quantitative (gentamicin protection assays and bacterial plating) and qualitative analysis (immunofluorescence microscopy) were performed. Bacterial colocalization with different markers of the endocytic pathway was examined to characterize its intracellular trafficking. Results : We found that S.aureus uptake requires host actin polymerization, microtubule assembly and activation of phosphatidylinositol 3-kinase signaling. Time course experiments showed that Newman strain was able to persist within macrophages at least until 28.5 h post infection. We observed that intracellular bacteria are located inside an acidic subcellular compartment, which co-localizes with the late endosome/lysosome markers Lamp-1, Rab7 and RILP. Colocalization counts with TMR-dextran might reflect a balance between bacterial killing and intracellular survival. Conclusions : This study indicates that S.aureus persists and replicates inside murine alveolar macrophages, representing a privileged niche that can potentially offer protection from antimicrobial activity and immunological host defense mechanisms.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/187835
https://dx.doi.org/urn:doi:10.1080/21505594.2017.1361089
url https://ddd.uab.cat/record/187835
https://dx.doi.org/urn:doi:10.1080/21505594.2017.1361089
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI13-01418
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 SEPAR054-2011
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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