Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide

13 pages. -- SI Figure 1: Characterization of rGO. (a) Raman spectra and (b) FTIR patterns of rGO. functionalized-rGO (green), rGO (red) and GO (black). -- SI Figure 2: Characterization of functionalized rGO.(a) XPS spectra of the functionalized rGO. Deconvolution of (b) C1s and (c) O1s.-- SI Figure...

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Autores: Chortarea, Savvina, Kuru, Ogul Can, Netkueakul, Woranan, Pelin, Marco, Keshavan, Sandeep, Song, Zhengmei, Ma, Baojin, Gómes, Julio, Villaro, Elvira, Visani de Luna, Luis Augusto, Loret, Thomas, Fordham, Alexander, Drummond, Matthew, Kontis, Nikolaos, Anagnostopoulos, George, Paterakis, George, Cataldi, Pietro, Tubaro, Aurelia, Galiotis, Costas, Kinloch, Ian, Fadeel, Bengt, Bussy, Cyrill, Kostarelos, Kostas, Buerki-Thurnherr, Tina, Prato, Maurizio, Bianco, Alberto, Wick, Peter
Tipo de recurso: conjunto de datos
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330216
Acceso en línea:http://hdl.handle.net/10261/330216
Access Level:acceso abierto
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dc.title.none.fl_str_mv Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
title Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
spellingShingle Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
Chortarea, Savvina
title_short Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
title_full Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
title_fullStr Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
title_full_unstemmed Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
title_sort Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene Oxide
dc.creator.none.fl_str_mv Chortarea, Savvina
Kuru, Ogul Can
Netkueakul, Woranan
Pelin, Marco
Keshavan, Sandeep
Song, Zhengmei
Ma, Baojin
Gómes, Julio
Villaro, Elvira
Visani de Luna, Luis Augusto
Loret, Thomas
Fordham, Alexander
Drummond, Matthew
Kontis, Nikolaos
Anagnostopoulos, George
Paterakis, George
Cataldi, Pietro
Tubaro, Aurelia
Galiotis, Costas
Kinloch, Ian
Fadeel, Bengt
Bussy, Cyrill
Kostarelos, Kostas
Buerki-Thurnherr, Tina
Prato, Maurizio
Bianco, Alberto
Wick, Peter
author Chortarea, Savvina
author_facet Chortarea, Savvina
Kuru, Ogul Can
Netkueakul, Woranan
Pelin, Marco
Keshavan, Sandeep
Song, Zhengmei
Ma, Baojin
Gómes, Julio
Villaro, Elvira
Visani de Luna, Luis Augusto
Loret, Thomas
Fordham, Alexander
Drummond, Matthew
Kontis, Nikolaos
Anagnostopoulos, George
Paterakis, George
Cataldi, Pietro
Tubaro, Aurelia
Galiotis, Costas
Kinloch, Ian
Fadeel, Bengt
Bussy, Cyrill
Kostarelos, Kostas
Buerki-Thurnherr, Tina
Prato, Maurizio
Bianco, Alberto
Wick, Peter
author_role author
author2 Kuru, Ogul Can
Netkueakul, Woranan
Pelin, Marco
Keshavan, Sandeep
Song, Zhengmei
Ma, Baojin
Gómes, Julio
Villaro, Elvira
Visani de Luna, Luis Augusto
Loret, Thomas
Fordham, Alexander
Drummond, Matthew
Kontis, Nikolaos
Anagnostopoulos, George
Paterakis, George
Cataldi, Pietro
Tubaro, Aurelia
Galiotis, Costas
Kinloch, Ian
Fadeel, Bengt
Bussy, Cyrill
Kostarelos, Kostas
Buerki-Thurnherr, Tina
Prato, Maurizio
Bianco, Alberto
Wick, Peter
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Wick, Peter [peter.wick@empa.ch]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 13 pages. -- SI Figure 1: Characterization of rGO. (a) Raman spectra and (b) FTIR patterns of rGO. functionalized-rGO (green), rGO (red) and GO (black). -- SI Figure 2: Characterization of functionalized rGO.(a) XPS spectra of the functionalized rGO. Deconvolution of (b) C1s and (c) O1s.-- SI Figure 3: TGA of functionalized rGO. -- SI Figure 4: TEM micrographs of rGO after the APTES functionalization. -- SI Figure 5: Physicochemical characterization of rGO and abraded composite materials. (a) AFM images of rGO and PA6-rGO . Lateral dimension and thickness distribution analysis for rGO and PA6-rGO. (b) Optical microscopy images of rGO, PA6-rGO and PA6. -- SI Figure 6: (a) EPR spectra of the powder of PA6, rGO and PA6-rGO at room temperature. (b)Assessment of ROS. Fluorescent spectra of PA6, rGO and PA6-rGO in 0.1 % BSA at 40 µg/mL in the presence of DHR123. Fenton reaction (Fe2+ + H2O2) corresponds to the positive control. -- EPR analysis of PA6, rGO and PA6-rGO. -- Fluorescence analysis of PA6, rGO and PA6-rGO. -- SI Figure 7: Particle size distributions of abraded (a) PA6-rGO and (b) PA6 particles in the nanometer range (13–573 nm) measured, measured by SMPS. The results show mean ± SD from at least three-independent measurements. -- SI Figure 8: TNF-α Expression Test (TET) for endotoxin detection in HMDMs exposed to PA6, PA6-rGO, and rGO (20 g/mL) or to bacterial LPS (0.01 µg/mL) in the presence or absence of the specific LPS inhibitor, polymyxin B (Poly-B; 10 µM) for 24h. . * indicates statistical significance compared to the negative control in absence of Poly-B (p < 0.05). # shows a statistically significant response (p < 0.05) in presence of Poly-B. -- SI Figure 9: (a) SEM images of the crack surface of the freeze-fractured samples of neat PA6 and PA6-rGO composite. Red line and red arrows indicate pulled-out rGOs. (b) TEM image of the abraded particles from the PA6-rGO composite showing the protruding rGOs from PA6 matrix marked with a dashed line. Layered structure of rGO can be observed at higher magnification. -- SI Figure 10: Pro-inflammatory response of A549 epithelial cells after treatment with the inflammogenic material DQ (100 µg/mL). (a) IL-6 and (b) IL-8 release measured after 24 h. Results are shown as mean ± standard error of the mean (SEM) from three independent experiments. * indicates statistical significance compared to the negative control at 24 h of exposure (p < 0.05). -- SI Figure 10: Pro-inflammatory response of A549 epithelial cells after treatment with the inflammogenic material DQ (100 µg/mL). (a) IL-6 and (b) IL-8 release measured after 24 h. Results are shown as mean ± standard error of the mean (SEM) from three independent experiments. * indicates statistical significance compared to the negative control at 24 h of exposure (p < 0.05). -- SI Figure 12: (a) Cytokine release by THP-1 macrophages. IFN-γ, TNF-α and IL-1β levels were determined after incubation with 20 µg/mL of PA6, PA6-rGO and rGO. Data are the mean ± SEM of 3 independent experiments. The * symbol represents p<0.05 as compared to the negative control. (b) THP-1 cells incubated with rGO (20 µg/mL) in the presence or absence of the pan-caspase inhibitor, zVAD-fmk (20 µM) or the NLRP3 inhibitor MCC950 (10 µM). * = p < 0.05, ** = p < 0.01, *** = p < 0.001. -- SI Figure 13: Cell morphology of RAW 264.7 macrophages exposed to different concentrations of rGO and abraded PA6 and PA6-rGO composites after 24 h. Scale bar: 50 μm. -- SI Figure 14: Cytokine profiling and hierarchical cluster analysis of inflammatory mediators released in differentiated HL-60 neutrophils exposed to rGO, abraded PA6-rGO and PA6. -- SI Figure 15: Interference assessment of the abraded particles (PA6 and PA6-rGO) and rGO with the MTS assay. -- SI Figure 16: : TNF-α and IL-6 measurements in the Bronchoalveolar lavage fluids.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330216
url http://hdl.handle.net/10261/330216
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chortarea, Savvina; Kuru, Ogul Can; Netkueakul, Woranan; Pelin, Marco; Keshavan, Sandeep; Song, Zhengmei; Ma, Baojin; Gómes, Julio; Villaro Abalos, Elvira; Visani de Luna, Luis Augusto; Loret, Thomas; Fordham, Alexander; Drummond, Matthew; Kontis, Nikolaos; Anagnostopoulos, George; Paterakis, George; Cataldi, Pietro; Tubaro, Aurelia; Galiotis, Costas; Kinloch, Ian; Fadeel, Bengt; Bussy, Cyrill; Kostarelos, Kostas; Buerki-Thurnherr, Tina; Prato, Maurizio; Bianco, Alberto; Wick, Peter. Hazard assessment of abraded thermoplastic composites reinforced with reduced graphene oxide. http://doi.org/10.1016/j.jhazmat.2022.129053. http://hdl.handle.net/10261/280660
http://doi.org/10.1016/j.jhazmat.2022.129053
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/msword
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418701669793792
spelling Supporting Information Hazard Assessment of Abraded Thermoplastic Composites Reinforced with Reduced Graphene OxideChortarea, SavvinaKuru, Ogul CanNetkueakul, WorananPelin, MarcoKeshavan, SandeepSong, ZhengmeiMa, BaojinGómes, JulioVillaro, ElviraVisani de Luna, Luis AugustoLoret, ThomasFordham, AlexanderDrummond, MatthewKontis, NikolaosAnagnostopoulos, GeorgePaterakis, GeorgeCataldi, PietroTubaro, AureliaGaliotis, CostasKinloch, IanFadeel, BengtBussy, CyrillKostarelos, KostasBuerki-Thurnherr, TinaPrato, MaurizioBianco, AlbertoWick, Peter13 pages. -- SI Figure 1: Characterization of rGO. (a) Raman spectra and (b) FTIR patterns of rGO. functionalized-rGO (green), rGO (red) and GO (black). -- SI Figure 2: Characterization of functionalized rGO.(a) XPS spectra of the functionalized rGO. Deconvolution of (b) C1s and (c) O1s.-- SI Figure 3: TGA of functionalized rGO. -- SI Figure 4: TEM micrographs of rGO after the APTES functionalization. -- SI Figure 5: Physicochemical characterization of rGO and abraded composite materials. (a) AFM images of rGO and PA6-rGO . Lateral dimension and thickness distribution analysis for rGO and PA6-rGO. (b) Optical microscopy images of rGO, PA6-rGO and PA6. -- SI Figure 6: (a) EPR spectra of the powder of PA6, rGO and PA6-rGO at room temperature. (b)Assessment of ROS. Fluorescent spectra of PA6, rGO and PA6-rGO in 0.1 % BSA at 40 µg/mL in the presence of DHR123. Fenton reaction (Fe2+ + H2O2) corresponds to the positive control. -- EPR analysis of PA6, rGO and PA6-rGO. -- Fluorescence analysis of PA6, rGO and PA6-rGO. -- SI Figure 7: Particle size distributions of abraded (a) PA6-rGO and (b) PA6 particles in the nanometer range (13–573 nm) measured, measured by SMPS. The results show mean ± SD from at least three-independent measurements. -- SI Figure 8: TNF-α Expression Test (TET) for endotoxin detection in HMDMs exposed to PA6, PA6-rGO, and rGO (20 g/mL) or to bacterial LPS (0.01 µg/mL) in the presence or absence of the specific LPS inhibitor, polymyxin B (Poly-B; 10 µM) for 24h. . * indicates statistical significance compared to the negative control in absence of Poly-B (p < 0.05). # shows a statistically significant response (p < 0.05) in presence of Poly-B. -- SI Figure 9: (a) SEM images of the crack surface of the freeze-fractured samples of neat PA6 and PA6-rGO composite. Red line and red arrows indicate pulled-out rGOs. (b) TEM image of the abraded particles from the PA6-rGO composite showing the protruding rGOs from PA6 matrix marked with a dashed line. Layered structure of rGO can be observed at higher magnification. -- SI Figure 10: Pro-inflammatory response of A549 epithelial cells after treatment with the inflammogenic material DQ (100 µg/mL). (a) IL-6 and (b) IL-8 release measured after 24 h. Results are shown as mean ± standard error of the mean (SEM) from three independent experiments. * indicates statistical significance compared to the negative control at 24 h of exposure (p < 0.05). -- SI Figure 10: Pro-inflammatory response of A549 epithelial cells after treatment with the inflammogenic material DQ (100 µg/mL). (a) IL-6 and (b) IL-8 release measured after 24 h. Results are shown as mean ± standard error of the mean (SEM) from three independent experiments. * indicates statistical significance compared to the negative control at 24 h of exposure (p < 0.05). -- SI Figure 12: (a) Cytokine release by THP-1 macrophages. IFN-γ, TNF-α and IL-1β levels were determined after incubation with 20 µg/mL of PA6, PA6-rGO and rGO. Data are the mean ± SEM of 3 independent experiments. The * symbol represents p<0.05 as compared to the negative control. (b) THP-1 cells incubated with rGO (20 µg/mL) in the presence or absence of the pan-caspase inhibitor, zVAD-fmk (20 µM) or the NLRP3 inhibitor MCC950 (10 µM). * = p < 0.05, ** = p < 0.01, *** = p < 0.001. -- SI Figure 13: Cell morphology of RAW 264.7 macrophages exposed to different concentrations of rGO and abraded PA6 and PA6-rGO composites after 24 h. Scale bar: 50 μm. -- SI Figure 14: Cytokine profiling and hierarchical cluster analysis of inflammatory mediators released in differentiated HL-60 neutrophils exposed to rGO, abraded PA6-rGO and PA6. -- SI Figure 15: Interference assessment of the abraded particles (PA6 and PA6-rGO) and rGO with the MTS assay. -- SI Figure 16: : TNF-α and IL-6 measurements in the Bronchoalveolar lavage fluids.Peer reviewedElsevierWick, Peter [peter.wick@empa.ch]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/mswordhttp://hdl.handle.net/10261/330216reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésChortarea, Savvina; Kuru, Ogul Can; Netkueakul, Woranan; Pelin, Marco; Keshavan, Sandeep; Song, Zhengmei; Ma, Baojin; Gómes, Julio; Villaro Abalos, Elvira; Visani de Luna, Luis Augusto; Loret, Thomas; Fordham, Alexander; Drummond, Matthew; Kontis, Nikolaos; Anagnostopoulos, George; Paterakis, George; Cataldi, Pietro; Tubaro, Aurelia; Galiotis, Costas; Kinloch, Ian; Fadeel, Bengt; Bussy, Cyrill; Kostarelos, Kostas; Buerki-Thurnherr, Tina; Prato, Maurizio; Bianco, Alberto; Wick, Peter. Hazard assessment of abraded thermoplastic composites reinforced with reduced graphene oxide. http://doi.org/10.1016/j.jhazmat.2022.129053. http://hdl.handle.net/10261/280660http://doi.org/10.1016/j.jhazmat.2022.129053Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3302162026-05-22T06:33:51Z
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