Expression of endothelial nitric oxide synthase in human peritoneal tissue: regulation by escherichia coli lipopolysaccharide

Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflammation. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 3′-untranslated...

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Detalles Bibliográficos
Autores: Arriero, María M., Rodríguez-Feo, Juan A., Celdrán, Ángel, Sánchez de Miguel, Lourdes, González Fernández, Fernando, Fortes, José, Reyero, Ana, Frieyro, Octavio, Pinta, Juan C de la, Franco, Ángeles, Casado, Santos, López Farre, Antonio José, Pastor Vargas, Carlos
Tipo de recurso: artículo
Fecha de publicación:2000
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/105612
Acceso en línea:https://hdl.handle.net/20.500.14352/105612
Access Level:acceso abierto
Palabra clave:577.1
Escherichia coli
Lipopolysaccharides
Nitric Oxide Synthase
Peritoneum
Ciencias Biomédicas
3205.06 Nefrología
Descripción
Sumario:Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflammation. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 3′-untranslated region (3′-UTR) of eNOS mRNA and could be implicated in eNOS mRNA stabilization. The present work demonstrates that eNOS protein is expressed in human endothelial and mesothelial peritoneal cells. <jats:italic toggle="yes">Escherichia coli</jats:italic> lipopolysaccharide shortened the half-life of eNOS message, reducing eNOS protein expression in peritoneal mesothelial and endothelial cells. Moreover, under basal conditions, human peritoneal samples expressed cytosolic proteins that bind to the 3′-UTR of eNOS mRNA. The cytosolic proteins that directly bind to 3′-UTR were identified as a 60-kD protein. After incubation of human peritoneal samples with lipopolysaccharide, the binding activity of the cytosolic 60-kD protein increased in a time-dependent manner. Studies are now necessary to determine the involvement of this 60-kD protein in the regulation of eNOS expression in peritoneal cells and particularly its involvement in the peritoneal dysfunction associated with inflammatory reactions.

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