Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice

Pregnancy is associated with hyperphagia, increased adiposity and multiple neuroendocrine adaptations. Maternal adipose tissue secretes rising amounts of interleukin 6 (IL6), which acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. To e...

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Autores: Pazos Mendoza, Patricia, Lima Rodríguez, Luis, Casanueva Freijo, Felipe, Diéguez González, Carlos, García García, María del Carmen
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/3685
Acceso en línea:http://hdl.handle.net/20.500.11940/3685
Access Level:acceso abierto
Palabra clave:Gene Expression Regulation
Hypothalamus
Interleukin-6
Thyrotropin-Releasing Hormone
Pro-Opiomelanocortin
Hipotálamo
Interleucina-6
Hormona Liberadora de Tirotropina
Regulación de la Expresión Génica
Proopiomelanocortina
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oai_identifier_str oai:runa.sergas.gal:20.500.11940/3685
network_acronym_str ES
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repository_id_str
spelling Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in MicePazos Mendoza, PatriciaLima Rodríguez, LuisCasanueva Freijo, FelipeDiéguez González, CarlosGarcía García, María del CarmenGene Expression RegulationHypothalamusInterleukin-6Thyrotropin-Releasing HormonePro-OpiomelanocortinHipotálamoInterleucina-6Hormona Liberadora de TirotropinaRegulación de la Expresión GénicaProopiomelanocortinaPregnancy is associated with hyperphagia, increased adiposity and multiple neuroendocrine adaptations. Maternal adipose tissue secretes rising amounts of interleukin 6 (IL6), which acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. To explore the role of IL6 in the central mechanisms governing dam's energy homeostasis, early, mid and late pregnant (gestational days 7, 13 and 18) wild-type (WT) and Il6 knockout mice (Il6-KO) were compared with virgin controls at diestrus. Food intake, body weight and composition as well as indirect calorimetry measurements were performed in vivo. Anabolic and orexigenic peptides: neuropeptide Y (Npy) and agouti-related peptide (Agrp); and catabolic and anorectic neuropeptides: proopiomelanocortin (Pomc), corticotrophin and thyrotropin-releasing hormone (Crh and Trh) mRNA levels were determined by in situ hybridization. Real time-PCR and western-blot were used for additional tissue gene expression and protein studies. Non-pregnant Il6-KO mice were leaner than WT mice due to a decrease in fat but not in lean body mass. Pregnant Il6-KO mice had higher fat accretion despite similar body weight gain than WT controls. A decreased fat utilization in absence of Il6 might explain this effect, as shown by increased respiratory exchange ratio (RER) in virgin Il6-KO mice. Il6 mRNA levels were markedly enhanced in adipose tissue but reduced in hypothalamus of mid and late pregnant WT mice. Trh expression was also stimulated at gestational day 13 and lack of Il6 blunted this effect. Conversely, in late pregnant mice lessened hypothalamic Il6 receptor alpha (Il6ra), Pomc and Crh mRNA were observed. Il6 deficiency during this stage up-regulated Npy and Agrp expression, while restoring Pomc mRNA levels to virgin values. Together these results demonstrate that IL6/IL6Ra system modulates Npy/Agrp, Pomc and Trh expression during mouse pregnancy, supporting a role of IL6 in the central regulation of body fat in this physiological state.2013info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.11940/3685reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/36852026-06-12T08:40:47Z
dc.title.none.fl_str_mv Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
title Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
spellingShingle Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
Pazos Mendoza, Patricia
Gene Expression Regulation
Hypothalamus
Interleukin-6
Thyrotropin-Releasing Hormone
Pro-Opiomelanocortin
Hipotálamo
Interleucina-6
Hormona Liberadora de Tirotropina
Regulación de la Expresión Génica
Proopiomelanocortina
title_short Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
title_full Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
title_fullStr Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
title_full_unstemmed Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
title_sort Interleukin 6 Deficiency Modulates the Hypothalamic Expression of Energy Balance Regulating Peptides during Pregnancy in Mice
dc.creator.none.fl_str_mv Pazos Mendoza, Patricia
Lima Rodríguez, Luis
Casanueva Freijo, Felipe
Diéguez González, Carlos
García García, María del Carmen
author Pazos Mendoza, Patricia
author_facet Pazos Mendoza, Patricia
Lima Rodríguez, Luis
Casanueva Freijo, Felipe
Diéguez González, Carlos
García García, María del Carmen
author_role author
author2 Lima Rodríguez, Luis
Casanueva Freijo, Felipe
Diéguez González, Carlos
García García, María del Carmen
author2_role author
author
author
author
dc.subject.none.fl_str_mv Gene Expression Regulation
Hypothalamus
Interleukin-6
Thyrotropin-Releasing Hormone
Pro-Opiomelanocortin
Hipotálamo
Interleucina-6
Hormona Liberadora de Tirotropina
Regulación de la Expresión Génica
Proopiomelanocortina
topic Gene Expression Regulation
Hypothalamus
Interleukin-6
Thyrotropin-Releasing Hormone
Pro-Opiomelanocortin
Hipotálamo
Interleucina-6
Hormona Liberadora de Tirotropina
Regulación de la Expresión Génica
Proopiomelanocortina
description Pregnancy is associated with hyperphagia, increased adiposity and multiple neuroendocrine adaptations. Maternal adipose tissue secretes rising amounts of interleukin 6 (IL6), which acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. To explore the role of IL6 in the central mechanisms governing dam's energy homeostasis, early, mid and late pregnant (gestational days 7, 13 and 18) wild-type (WT) and Il6 knockout mice (Il6-KO) were compared with virgin controls at diestrus. Food intake, body weight and composition as well as indirect calorimetry measurements were performed in vivo. Anabolic and orexigenic peptides: neuropeptide Y (Npy) and agouti-related peptide (Agrp); and catabolic and anorectic neuropeptides: proopiomelanocortin (Pomc), corticotrophin and thyrotropin-releasing hormone (Crh and Trh) mRNA levels were determined by in situ hybridization. Real time-PCR and western-blot were used for additional tissue gene expression and protein studies. Non-pregnant Il6-KO mice were leaner than WT mice due to a decrease in fat but not in lean body mass. Pregnant Il6-KO mice had higher fat accretion despite similar body weight gain than WT controls. A decreased fat utilization in absence of Il6 might explain this effect, as shown by increased respiratory exchange ratio (RER) in virgin Il6-KO mice. Il6 mRNA levels were markedly enhanced in adipose tissue but reduced in hypothalamus of mid and late pregnant WT mice. Trh expression was also stimulated at gestational day 13 and lack of Il6 blunted this effect. Conversely, in late pregnant mice lessened hypothalamic Il6 receptor alpha (Il6ra), Pomc and Crh mRNA were observed. Il6 deficiency during this stage up-regulated Npy and Agrp expression, while restoring Pomc mRNA levels to virgin values. Together these results demonstrate that IL6/IL6Ra system modulates Npy/Agrp, Pomc and Trh expression during mouse pregnancy, supporting a role of IL6 in the central regulation of body fat in this physiological state.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.11940/3685
url http://hdl.handle.net/20.500.11940/3685
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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