'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure

The main scope of this manuscript is to analyse the dynamics of mitochondrial activity in boar sperm subjected to 'in vitro' capacitation (IVC) and subsequent progesterone-induced 'in vitro' acrosome reaction (IVAR). This was determined after analysis of the rhythm of O(2) consum...

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Autores: Ramió-Lluch, Laura, Fernández Novell, Josep M.|||0000-0002-5349-8303, Peña, Alejandro, Colás, C., Cebrián-Pérez, José A.|||0000-0001-9842-6197, Muiño-Blanco, T., Ramírez, A., Concha, Ilona I.|||0000-0001-6345-1878, Rigau i Mas, Teresa|||0000-0002-2688-0275, Rodríguez Gil, Joan Enric|||0000-0002-1112-9884
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:catalán
OAI Identifier:oai:ddd.uab.cat:123264
Acceso en línea:https://ddd.uab.cat/record/123264
https://dx.doi.org/urn:doi:10.1111/j.1439-0531.2010.01655.x
Access Level:acceso abierto
Palabra clave:Senglars
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spelling 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structureRamió-Lluch, LauraFernández Novell, Josep M.|||0000-0002-5349-8303Peña, AlejandroColás, C.Cebrián-Pérez, José A.|||0000-0001-9842-6197Muiño-Blanco, T.Ramírez, A.Concha, Ilona I.|||0000-0001-6345-1878Rigau i Mas, Teresa|||0000-0002-2688-0275Rodríguez Gil, Joan Enric|||0000-0002-1112-9884SenglarsThe main scope of this manuscript is to analyse the dynamics of mitochondrial activity in boar sperm subjected to 'in vitro' capacitation (IVC) and subsequent progesterone-induced 'in vitro' acrosome reaction (IVAR). This was determined after analysis of the rhythm of O(2) consumption and concomitant changes in the mitochondria activity-specific JC-1 staining. Results showed that IVC, and especially IVAR, was concomitant with a peak in O(2) consumption (from 1.61 ± 0.08 nmol O(2)/min/10(7) viable sperm at 0 h of incubation to 2.62 ± 0.12 nmol O(2) /min/10(7) viable sperm after 5 min of IVAR induction). These results were accompanied by parallel changes in the mean intensity of JC-1 staining. Based on JC-1, mitochondrial activation followed a nucleated pattern, with specific, activation starting points at the midpiece from which mitochondrial activation was spread. Moreover, four separate sperm subpopulations were detected following the JC-1 orange-red/green ratio, and the observed changes in the mean JC-1 staining during IVC and IVAR were related to concomitant changes in both the orange-red/green JC-1 ratio and the percentage of sperm included in each subpopulation. All of these results indicate that IVC and the first minutes of IVAR are accompanied by a progressive increase in mitochondrial activity, which reached a peak coincidental with the achievement of IVAR. Moreover, results suggest the presence of separate sperm subpopulations, which show a different mitochondrial sensitivity to IVC and IVAR. Finally, mitochondrial activation, at least under JC-1 staining, seems to originate in concrete nucleation points at the midpiece, thus suggesting thus a well-coordinated pattern in boar-sperm mitochondrial activity modulation. 22010-01-0120102010-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/123264https://dx.doi.org/urn:doi:10.1111/j.1439-0531.2010.01655.xreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaCataláncatMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2004-04756-C02/02/GANMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2008-01792GANopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1232642026-06-06T12:50:31Z
dc.title.none.fl_str_mv 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
title 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
spellingShingle 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
Ramió-Lluch, Laura
Senglars
title_short 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
title_full 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
title_fullStr 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
title_full_unstemmed 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
title_sort 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
dc.creator.none.fl_str_mv Ramió-Lluch, Laura
Fernández Novell, Josep M.|||0000-0002-5349-8303
Peña, Alejandro
Colás, C.
Cebrián-Pérez, José A.|||0000-0001-9842-6197
Muiño-Blanco, T.
Ramírez, A.
Concha, Ilona I.|||0000-0001-6345-1878
Rigau i Mas, Teresa|||0000-0002-2688-0275
Rodríguez Gil, Joan Enric|||0000-0002-1112-9884
author Ramió-Lluch, Laura
author_facet Ramió-Lluch, Laura
Fernández Novell, Josep M.|||0000-0002-5349-8303
Peña, Alejandro
Colás, C.
Cebrián-Pérez, José A.|||0000-0001-9842-6197
Muiño-Blanco, T.
Ramírez, A.
Concha, Ilona I.|||0000-0001-6345-1878
Rigau i Mas, Teresa|||0000-0002-2688-0275
Rodríguez Gil, Joan Enric|||0000-0002-1112-9884
author_role author
author2 Fernández Novell, Josep M.|||0000-0002-5349-8303
Peña, Alejandro
Colás, C.
Cebrián-Pérez, José A.|||0000-0001-9842-6197
Muiño-Blanco, T.
Ramírez, A.
Concha, Ilona I.|||0000-0001-6345-1878
Rigau i Mas, Teresa|||0000-0002-2688-0275
Rodríguez Gil, Joan Enric|||0000-0002-1112-9884
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Senglars
topic Senglars
description The main scope of this manuscript is to analyse the dynamics of mitochondrial activity in boar sperm subjected to 'in vitro' capacitation (IVC) and subsequent progesterone-induced 'in vitro' acrosome reaction (IVAR). This was determined after analysis of the rhythm of O(2) consumption and concomitant changes in the mitochondria activity-specific JC-1 staining. Results showed that IVC, and especially IVAR, was concomitant with a peak in O(2) consumption (from 1.61 ± 0.08 nmol O(2)/min/10(7) viable sperm at 0 h of incubation to 2.62 ± 0.12 nmol O(2) /min/10(7) viable sperm after 5 min of IVAR induction). These results were accompanied by parallel changes in the mean intensity of JC-1 staining. Based on JC-1, mitochondrial activation followed a nucleated pattern, with specific, activation starting points at the midpiece from which mitochondrial activation was spread. Moreover, four separate sperm subpopulations were detected following the JC-1 orange-red/green ratio, and the observed changes in the mean JC-1 staining during IVC and IVAR were related to concomitant changes in both the orange-red/green JC-1 ratio and the percentage of sperm included in each subpopulation. All of these results indicate that IVC and the first minutes of IVAR are accompanied by a progressive increase in mitochondrial activity, which reached a peak coincidental with the achievement of IVAR. Moreover, results suggest the presence of separate sperm subpopulations, which show a different mitochondrial sensitivity to IVC and IVAR. Finally, mitochondrial activation, at least under JC-1 staining, seems to originate in concrete nucleation points at the midpiece, thus suggesting thus a well-coordinated pattern in boar-sperm mitochondrial activity modulation.
publishDate 2010
dc.date.none.fl_str_mv 2
2010-01-01
2010
2010-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/123264
https://dx.doi.org/urn:doi:10.1111/j.1439-0531.2010.01655.x
url https://ddd.uab.cat/record/123264
https://dx.doi.org/urn:doi:10.1111/j.1439-0531.2010.01655.x
dc.language.none.fl_str_mv Catalán
cat
language_invalid_str_mv Catalán
language cat
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2004-04756-C02/02/GAN
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2008-01792GAN
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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