Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences, represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA v...

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Detalles Bibliográficos
Autores: Almazán, Fernando, Márquez-Jurado, Silvia, Nogales, Aitor, Enjuanes Sánchez, Luis
Tipo de recurso: otro
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/204175
Acceso en línea:http://hdl.handle.net/10261/204175
Access Level:acceso abierto
Palabra clave:Coronavirus
MERS
Reverse genetics
Infectious clones
Bacterial artificial chromosomes
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spelling Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial ChromosomesAlmazán, FernandoMárquez-Jurado, SilviaNogales, AitorEnjuanes Sánchez, LuisCoronavirusMERSReverse geneticsInfectious clonesBacterial artificial chromosomesThe large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences, represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last thirteen years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.This work was supported by grants from the Ministry of Science and Innovation of Spain (MCINN) (BIO2010-16705), the European Community's Seventh Framework Programme (FP7/2007-2013) under the project “EMPERIE” (HEALTH-F3-2009-223498), and the National Institute of Health (NIH) of USA (2P01AI060699-06A1). S. M. received a predoctoral fellowship from the National Institute of Health (ISCIII) of Spain.Humana PressSpringer NatureMinisterio de Ciencia e Innovación (España)European CommissionNational Institutes of Health (US)Instituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020152020info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248info:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/204175reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/223498https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726977/Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2041752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
spellingShingle Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
Almazán, Fernando
Coronavirus
MERS
Reverse genetics
Infectious clones
Bacterial artificial chromosomes
title_short Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_full Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_fullStr Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_full_unstemmed Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
title_sort Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
dc.creator.none.fl_str_mv Almazán, Fernando
Márquez-Jurado, Silvia
Nogales, Aitor
Enjuanes Sánchez, Luis
author Almazán, Fernando
author_facet Almazán, Fernando
Márquez-Jurado, Silvia
Nogales, Aitor
Enjuanes Sánchez, Luis
author_role author
author2 Márquez-Jurado, Silvia
Nogales, Aitor
Enjuanes Sánchez, Luis
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
National Institutes of Health (US)
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Coronavirus
MERS
Reverse genetics
Infectious clones
Bacterial artificial chromosomes
topic Coronavirus
MERS
Reverse genetics
Infectious clones
Bacterial artificial chromosomes
description The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences, represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last thirteen years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.
publishDate 2015
dc.date.none.fl_str_mv 2015
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/204175
url http://hdl.handle.net/10261/204175
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/223498
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726977/
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Humana Press
Springer Nature
publisher.none.fl_str_mv Humana Press
Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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