Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells
Breast cancer accounts for up to 10% of the newly diagnosed cancer cases worldwide, making it the most common cancer found in women. The use of superparamagnetic iron oxide nanoparticles (SPIONs) has been beneficial in the advancement of contrast agents and magnetic hyperthermia (MH) for the diagnos...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/269715 |
| Acceso en línea: | http://hdl.handle.net/10261/269715 https://api.elsevier.com/content/abstract/scopus_id/85128156627 |
| Access Level: | acceso abierto |
| Palabra clave: | Iron-oxide nanoparticles Particle-size Citric-acid Hyperthermia |
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Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| title |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| spellingShingle |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells Mekseriwattana, Wid Iron-oxide nanoparticles Particle-size Citric-acid Hyperthermia |
| title_short |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| title_full |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| title_fullStr |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| title_full_unstemmed |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| title_sort |
Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells |
| dc.creator.none.fl_str_mv |
Mekseriwattana, Wid Guardia, Pablo Torres Herrero, Beatriz Fuente, Jesús M. de la Kuhakarn, Chutima Roig Serra, Anna Katewongsa, Kanlaya Prapainop |
| author |
Mekseriwattana, Wid |
| author_facet |
Mekseriwattana, Wid Guardia, Pablo Torres Herrero, Beatriz Fuente, Jesús M. de la Kuhakarn, Chutima Roig Serra, Anna Katewongsa, Kanlaya Prapainop |
| author_role |
author |
| author2 |
Guardia, Pablo Torres Herrero, Beatriz Fuente, Jesús M. de la Kuhakarn, Chutima Roig Serra, Anna Katewongsa, Kanlaya Prapainop |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Mahidol University National Research Council of Thailand Ministry of Higher Education, Science, Research and Innovation (Thailand) Ministerio de Ciencia, Innovación y Universidades (España) European Commission Center of Excellence for Innovation in Chemistry (Thailand) Ministerio de Ciencia, Innovación y Universidades (España) Diputación General de Aragón Agencia Estatal de Investigación (España) Mekseriwattana, Wid [0000-0002-0966-6293] Torres Herrero, Beatriz 0000-0003-4648-8972] Kuhakarn, Chutima [0000-0003-4638-4356] Roig Serra, Anna [0000-0001-6464-7573] Katewongsa, Kanlaya Prapainop [0000-0001-6417-4921] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Iron-oxide nanoparticles Particle-size Citric-acid Hyperthermia |
| topic |
Iron-oxide nanoparticles Particle-size Citric-acid Hyperthermia |
| description |
Breast cancer accounts for up to 10% of the newly diagnosed cancer cases worldwide, making it the most common cancer found in women. The use of superparamagnetic iron oxide nanoparticles (SPIONs) has been beneficial in the advancement of contrast agents and magnetic hyperthermia (MH) for the diagnosis and treatment of cancers. To achieve delivery of SPIONs to cancer cells, surface functionalization with specific ligands are required. Riboflavin carrier protein (RCP) has been identified as an alternative target for breast cancer cells. Here, we report a novel riboflavin (Rf)-based ligand that provides SPIONs with enhanced colloidal stability and high uptake potential in breast cancer cells. This is achieved by synthesizing an Rf-citrate ligand. The ligand was tested in a multicore SPION system, and affinity to RCP was assessed by isothermal titration calorimetry which showed a specific, entropy-driven binding. MRI and MH responses of the coated Rf-SPIONs were tested to evaluate the suitability of this system as a theranostic platform. Finally, interaction of the Rf-SPIONs with breast cancer cells was evaluated by in vitro cellular uptake in MCF-7 breast cancer cells. The overall characterization of the Rf-SPIONs highlighted the excellent performance of this platform for theranostic applications in breast cancer. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/269715 https://api.elsevier.com/content/abstract/scopus_id/85128156627 |
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http://hdl.handle.net/10261/269715 https://api.elsevier.com/content/abstract/scopus_id/85128156627 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Royal Society of Chemistry (UK) |
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Royal Society of Chemistry (UK) |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869418648049811456 |
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Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cellsMekseriwattana, WidGuardia, PabloTorres Herrero, BeatrizFuente, Jesús M. de laKuhakarn, ChutimaRoig Serra, AnnaKatewongsa, Kanlaya PrapainopIron-oxide nanoparticlesParticle-sizeCitric-acidHyperthermiaBreast cancer accounts for up to 10% of the newly diagnosed cancer cases worldwide, making it the most common cancer found in women. The use of superparamagnetic iron oxide nanoparticles (SPIONs) has been beneficial in the advancement of contrast agents and magnetic hyperthermia (MH) for the diagnosis and treatment of cancers. To achieve delivery of SPIONs to cancer cells, surface functionalization with specific ligands are required. Riboflavin carrier protein (RCP) has been identified as an alternative target for breast cancer cells. Here, we report a novel riboflavin (Rf)-based ligand that provides SPIONs with enhanced colloidal stability and high uptake potential in breast cancer cells. This is achieved by synthesizing an Rf-citrate ligand. The ligand was tested in a multicore SPION system, and affinity to RCP was assessed by isothermal titration calorimetry which showed a specific, entropy-driven binding. MRI and MH responses of the coated Rf-SPIONs were tested to evaluate the suitability of this system as a theranostic platform. Finally, interaction of the Rf-SPIONs with breast cancer cells was evaluated by in vitro cellular uptake in MCF-7 breast cancer cells. The overall characterization of the Rf-SPIONs highlighted the excellent performance of this platform for theranostic applications in breast cancer.This project was supported by Mahidol University under a New Discovery and Frontier Research Grant to K. P. K. This project is funded by National Research Council of Thailand (NRCT) and Mahidol University: N42A650357. Partial support was also provided by a Central Instrument Facility (CIF) grant and the Center for Nanoimaging, Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation, Faculty of Science, Mahidol University. W. M. was supported by the Science Achievement Scholarship of Thailand. The authors would like to thank Associate Professor Nuttawee Niamsiri, Department of Biotechnology, Faculty of Science, Mahidol University, for the use of the Zetasizer instrument. This research was also funded by the Spanish Government, through the “Severo Ochoa” Programme for Centers of Excellence in R&D (FUNFUTURE, CEX2019-000917 S) and DGA, through the “Fondo Social Europeo” (DGA E15_20R). B. T. H. is grateful for a predoctoral fellowship FPU (FPU19/01311). P. G. acknowledges financial support from the Spanish government (MICIU) through the Ramon y Cajal research program (RyC2019-028414-I).Peer reviewedRoyal Society of Chemistry (UK)Mahidol UniversityNational Research Council of ThailandMinistry of Higher Education, Science, Research and Innovation (Thailand)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionCenter of Excellence for Innovation in Chemistry (Thailand)Ministerio de Ciencia, Innovación y Universidades (España)Diputación General de AragónAgencia Estatal de Investigación (España)Mekseriwattana, Wid [0000-0002-0966-6293]Torres Herrero, Beatriz 0000-0003-4648-8972]Kuhakarn, Chutima [0000-0003-4638-4356]Roig Serra, Anna [0000-0001-6464-7573]Katewongsa, Kanlaya Prapainop [0000-0001-6417-4921]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/269715https://api.elsevier.com/content/abstract/scopus_id/85128156627reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//CEX2019-000917-Sinfo:eu-repo/grantAgreement/AEI//RyC2019-028414-INanoscale AdvancesThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1039/d2na00015fhttp://dx.doi.org/10.1039/d2na00015fSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2697152026-05-22T06:33:51Z |
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15,812429 |