Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model

Age-related macular degeneration; Neovascular; Retina

Detalles Bibliográficos
Autores: Salas Torras, Anna, Badia Pérez, Anna, Fontrodona Montals, Laura, Zapata, Miguel Angel, Garcia-Arumi, Jose, Duarri, Anna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Departament de Salut de la Generalitat de Catalunya (DS)
Repositorio:Scientia. Dipòsit d'Informació Digital del Departament de Salut
OAI Identifier:oai:scientiasalut.gencat.cat:11351/10414
Acceso en línea:https://hdl.handle.net/11351/10414
Access Level:acceso abierto
Palabra clave:Ratolins
Degeneració macular
Angiogènesi
Coroide - Malalties
ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice
DISEASES::Eye Diseases::Uveal Diseases::Choroid Diseases::Choroidal Neovascularization
DISEASES::Eye Diseases::Retinal Diseases::Retinal Degeneration::Macular Degeneration
ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones
ENFERMEDADES::oftalmopatías::enfermedades de la úvea::enfermedades de la coroides::neovascularización de la coroides
ENFERMEDADES::oftalmopatías::enfermedades de la retina::degeneración retiniana::degeneración macular
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oai_identifier_str oai:scientiasalut.gencat.cat:11351/10414
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
title Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
spellingShingle Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
Salas Torras, Anna
Ratolins
Degeneració macular
Angiogènesi
Coroide - Malalties
ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice
DISEASES::Eye Diseases::Uveal Diseases::Choroid Diseases::Choroidal Neovascularization
DISEASES::Eye Diseases::Retinal Diseases::Retinal Degeneration::Macular Degeneration
ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones
ENFERMEDADES::oftalmopatías::enfermedades de la úvea::enfermedades de la coroides::neovascularización de la coroides
ENFERMEDADES::oftalmopatías::enfermedades de la retina::degeneración retiniana::degeneración macular
title_short Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
title_full Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
title_fullStr Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
title_full_unstemmed Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
title_sort Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model
dc.creator.none.fl_str_mv Salas Torras, Anna
Badia Pérez, Anna
Fontrodona Montals, Laura
Zapata, Miguel Angel
Garcia-Arumi, Jose
Duarri, Anna
author Salas Torras, Anna
author_facet Salas Torras, Anna
Badia Pérez, Anna
Fontrodona Montals, Laura
Zapata, Miguel Angel
Garcia-Arumi, Jose
Duarri, Anna
author_role author
author2 Badia Pérez, Anna
Fontrodona Montals, Laura
Zapata, Miguel Angel
Garcia-Arumi, Jose
Duarri, Anna
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Salas A, Badia A, Fontrodona L, Duarri A] Grup de Recerca Oftalmològica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Zapata M, García-Arumí J] Grup de Recerca Oftalmològica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Oftalmologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Ratolins
Degeneració macular
Angiogènesi
Coroide - Malalties
ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice
DISEASES::Eye Diseases::Uveal Diseases::Choroid Diseases::Choroidal Neovascularization
DISEASES::Eye Diseases::Retinal Diseases::Retinal Degeneration::Macular Degeneration
ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones
ENFERMEDADES::oftalmopatías::enfermedades de la úvea::enfermedades de la coroides::neovascularización de la coroides
ENFERMEDADES::oftalmopatías::enfermedades de la retina::degeneración retiniana::degeneración macular
topic Ratolins
Degeneració macular
Angiogènesi
Coroide - Malalties
ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice
DISEASES::Eye Diseases::Uveal Diseases::Choroid Diseases::Choroidal Neovascularization
DISEASES::Eye Diseases::Retinal Diseases::Retinal Degeneration::Macular Degeneration
ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones
ENFERMEDADES::oftalmopatías::enfermedades de la úvea::enfermedades de la coroides::neovascularización de la coroides
ENFERMEDADES::oftalmopatías::enfermedades de la retina::degeneración retiniana::degeneración macular
description Age-related macular degeneration; Neovascular; Retina
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/10414
url https://hdl.handle.net/11351/10414
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biomedicines;11(9)
https://doi.org/10.3390/biomedicines11092445
info:eu-repo/grantAgreement/ES/PE2013-2016/PI18%2F00219
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Scientia
reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut
instname:Departament de Salut de la Generalitat de Catalunya (DS)
instname_str Departament de Salut de la Generalitat de Catalunya (DS)
reponame_str Scientia. Dipòsit d'Informació Digital del Departament de Salut
collection Scientia. Dipòsit d'Informació Digital del Departament de Salut
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418635450122240
spelling Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse ModelSalas Torras, AnnaBadia Pérez, AnnaFontrodona Montals, LauraZapata, Miguel Angel Garcia-Arumi, JoseDuarri, AnnaRatolinsDegeneració macularAngiogènesiCoroide - MalaltiesORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::MiceDISEASES::Eye Diseases::Uveal Diseases::Choroid Diseases::Choroidal NeovascularizationDISEASES::Eye Diseases::Retinal Diseases::Retinal Degeneration::Macular DegenerationORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratonesENFERMEDADES::oftalmopatías::enfermedades de la úvea::enfermedades de la coroides::neovascularización de la coroidesENFERMEDADES::oftalmopatías::enfermedades de la retina::degeneración retiniana::degeneración macularAge-related macular degeneration; Neovascular; RetinaDegeneración macular asociada a la edad; Neovascular; RetinaDegeneració macular associada a l'edat; Neovascular; RetinaThe mouse model of laser-induced choroidal neovascularization (LI-CNV) has been widely used to study neovascular age-related macular degeneration; however, it still lacks a comprehensive characterization. Here, CNV was induced in the eyes of 12-week-old C57BL/6J male mice by argon laser irradiation. We studied the CNV lesion progression of an LI-CNV mouse cohort by using multimodal imaging (color fundus, optical coherence tomography (OCT), and fluorescence angiography, focal electroretinography features for 14 days, and related cytokines, angiogenic factors, and reactive gliosis for 5 days. CNV lesions involving the rupture of the Bruch’s membrane were confirmed using funduscopy and OCT after laser photocoagulation. During the initial stage, from the CNV induction until day 7, CNV lesions presented leakage observed by using fluorescence angiography and a typical hyperreflective area with cell infiltration, subretinal leakage, and degeneration of photoreceptors observed through OCT. This correlated with decreased retinal responses to light. Moreover, inflammatory and angiogenic markers were reduced to basal levels in the first 5 days of CNV progression. In contrast, reactive gliosis and the VEGF expression in retinal sections were sustained, with infiltration of endothelial cells in the subretinal space. In the second stage, between days 7 and 14 post-induction, we observed stabilization of the CNV lesions, a hyperfluorescent area corresponding to the formation of fibrosis, and a partial rescue of retinal function. These findings suggest that the LI-CNV lesion development goes through an acute phase during the first seven days following induction, and then the CNV lesion stabilizes. According to these results, this model is suitable for screening anti-inflammatory and anti-angiogenic drugs in the early stages of LI-CNV. At the same time, it is more convenient for screening anti-fibrotic compounds in the later stages.This research was funded by Instituto de Salud Carlos III, grant number PI13/0096 and PI18/00219, a predoctoral grant from Fundació Josep Palau Francàs.MDPIInstitut Català de la Salut[Salas A, Badia A, Fontrodona L, Duarri A] Grup de Recerca Oftalmològica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Zapata M, García-Arumí J] Grup de Recerca Oftalmològica, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’Oftalmologia, Vall d’Hebron Hospital Universitari, Barcelona, SpainVall d'Hebron Barcelona Hospital Campus202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/10414Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésBiomedicines;11(9)https://doi.org/10.3390/biomedicines11092445info:eu-repo/grantAgreement/ES/PE2013-2016/PI18%2F00219Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/104142026-06-12T09:38:37Z
score 15,81155

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