The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition

231 p.

Detalles Bibliográficos
Autor: Poucet, Théo Jean
Tipo de recurso: tesis doctoral
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/49822
Acceso en línea:http://hdl.handle.net/10810/49822
Access Level:acceso abierto
Palabra clave:molecular biology of plants
plant cytology
plant nutrition
biología molecular de plantas
citología vegetal
nutrición vegetal
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oai_identifier_str oai:addi.ehu.eus:10810/49822
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
title The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
spellingShingle The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
Poucet, Théo Jean
molecular biology of plants
plant cytology
plant nutrition
biología molecular de plantas
citología vegetal
nutrición vegetal
title_short The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
title_full The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
title_fullStr The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
title_full_unstemmed The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
title_sort The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutrition
dc.creator.none.fl_str_mv Poucet, Théo Jean
author Poucet, Théo Jean
author_facet Poucet, Théo Jean
author_role author
dc.contributor.none.fl_str_mv Marino Bilbao, Daniel
Dieuaide-Noubhani, Martine
dc.subject.none.fl_str_mv molecular biology of plants
plant cytology
plant nutrition
biología molecular de plantas
citología vegetal
nutrición vegetal
topic molecular biology of plants
plant cytology
plant nutrition
biología molecular de plantas
citología vegetal
nutrición vegetal
description 231 p.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/49822
url http://hdl.handle.net/10810/49822
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
(c)2020 THEO JEAN POUCET
eu_rights_str_mv openAccess
rights_invalid_str_mv (c)2020 THEO JEAN POUCET
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418633835315200
spelling The energy cost of primary metabolism vacuole expansion: central to shape toamto leaf development under ammonium nutritionPoucet, Théo Jeanmolecular biology of plantsplant cytologyplant nutritionbiología molecular de plantascitología vegetalnutrición vegetal231 p.Ammonium (NH4+) is a nitrogen source of great interest in the context of sustainable agriculture. Its application in the field together with nitrification inhibitors has been extensively proven efficient to limit detrimental N losses compared to the use of nitrate (N03). NH4+ is a common intermediate involved in numerous metabolic routes. However, high NH4 concentrations may lead to a stress situation provoking a set of symptoms collectively known as "ammonium syndrome" mainly characterized by growth retardation. Those symptoms are caused by a combination of, among others, a profound metabolic reprogramming, disruption of photosynthesis, pH deregulation and ion imbalance. Numerous studies have described the way plant copes to ammonium nutrition. However, the organ developmental stage has been generally neglected.To fill in this gap, in the first chapter we first aimed studying how the metabolism is adapted in function of the leaf position in the vertical axis of the tomato plants (Solanum lycopersicum) grown with NH4+, N03- or NH4N03 supply. To do so, we dissected leaf biomass composition and metabolism through a complete analysis of metabolites, ions and enzyme activities. The results showed that C and N metabolic adjustment in function of the nitrogen source was more intense in older leaves compared to younger ones. Importantly, we propose a trade-off between NH4+ accumulation and assimilation to preserve young leaves from ammonium stress. Besides, NH4+-fed plants exhibited a rearrangement of carbon skeletons with a higher energy cost respect to plants supplied with N03-. We explain such reallocation by the action of the biochemical pH-stat, to compensate the differential proton production that depends on the nitrogen form provided.Ammonium nutrition may limit cell expansion, suggesting that the cellular processes involved would be altered. Among others, cell growth is largely dependent of the internal pressure exerted on the cell wall by the vacuole. However, the role of the vacuole in ammonium stress has been rarely addressed. In the second chapter, we evaluated the effect of ammonium stress on leaf development with a special focus on vacuole expansion and metabolism. To carry out this aim, we monitored the leaf development from its appearance until its complete expansion in plants grown under NH4+ or NO/ as unique nitrogen source. Cytological analysis evidenced that the reduced cell expansion under ammonium nutrition was associated with smaller vacuole size. Besides, we reported an acidification of the vacuole of NH4+-fed plants compared to nitrate nutrition. Moreover, a model was built to predict the thermodynamic equilibrium of different soluble species across the tonoplast. The model was set up through an extensive reviewing of vacuolar transporters and integrated subcellular volumes, vacuolar electrochemical gradients and the formation of ionic complex in the vacuole to fit the subcellular concentration of ions, organic acids and sugars measured in the leaf. Further, predictions obtained with the model were cross validated with data from non-aqueous fractionation. Firstly, the entrance of solutes was higher in vacuoles of N03--fed leaves but was not associated with higher vacuolar osmolarity likely because of the adjustment of the vacuolar volume. In this sense, we proposed that the lack of malate in cells of ammonium-fed leaves was central in the limitation of vacuolar expansion. Secondly, we conclude that the energy cost of solute transport into the vacuole is higher under NH4+ based nutrition because of the higher electrochemical gradient generated by the proton pumps across tonoplast.This work highlights the importance of considering leaf phenological state when studying nitrogen metabolism. In addition, our integrated approach place cytosolic pH control and vacuole expansion in the center of tomato leaf adaptation to ammonium stress and pave the way for future studies in the field of ammonium nutrition.Marino Bilbao, DanielDieuaide-Noubhani, Martine2021202120202020info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10810/49822reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/semantics/openAccess(c)2020 THEO JEAN POUCEToai:addi.ehu.eus:10810/498222026-06-18T09:23:17Z
score 15,300719

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