Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]

[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Curre...

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Autores: Pan, Kyra van der, Khatri, Indu, Jager, Anniek L. de, Louis, Alesha, Kassem, Sara, Naber, Brigitta A. E., Laat, Inge F. de, Hameetman, Marjolijn, Comans, Suzanne, Orfao, Alberto, Dongen, J. J. M. van, Díez, Paula, Teodosio, Cristina
Tipo de documento: conjunto de datos
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/360921
Acesso em linha:http://hdl.handle.net/10261/360921
Access Level:Acceso aberto
Palavra-chave:Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
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spelling Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]Pan, Kyra van derKhatri, InduJager, Anniek L. deLouis, AleshaKassem, SaraNaber, Brigitta A. E.Laat, Inge F. deHameetman, MarjolijnComans, SuzanneOrfao, AlbertoDongen, J. J. M. vanDíez, PaulaTeodosio, CristinaSpectral flow cytometryMyeloid cellsImmunophenotypingcyTOFMass cytometry[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations.[Methods]: We evaluated the performance of two SFC and MC panels, which contained 21 common markers, for the identification and subsetting of blood IMC populations. Based on unsupervised clustering analysis, we systematically identified 24 leukocyte populations, including 21 IMC subsets, regardless of the cytometry technique.[Results]: Overall, comparable results were observed between the two technologies regarding the relative distribution of these cell populations and the staining resolution of individual markers (Pearson’s ρ=0.99 and 0.55, respectively). However, minor differences were observed between the two techniques regarding intra-measurement variability (median coefficient of variation of 42.5% vs. 68.0% in SFC and MC, respectively; p<0.0001) and reproducibility, which were most likely due to the significantly longer acquisition times (median 16 min vs. 159 min) and lower recovery rates (median 53.1% vs. 26.8%) associated with SFC vs. MC.[Discussion]: Altogether, our results show a good correlation between SFC and MC for the identification, enumeration and characterization of IMC in blood, based on large panels (>20) of antibody reagents.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionimage/tiffhttp://hdl.handle.net/10261/360921reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina. Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations. https://doi.org/10.3389/fimmu.2023.1191992 . http://hdl.handle.net/10261/348256https://doi.org/10.3389/fimmu.2023.1191992.s002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3609212026-05-22T06:33:51Z
dc.title.none.fl_str_mv Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
title Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
spellingShingle Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
Pan, Kyra van der
Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
title_short Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
title_full Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
title_fullStr Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
title_full_unstemmed Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
title_sort Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]
dc.creator.none.fl_str_mv Pan, Kyra van der
Khatri, Indu
Jager, Anniek L. de
Louis, Alesha
Kassem, Sara
Naber, Brigitta A. E.
Laat, Inge F. de
Hameetman, Marjolijn
Comans, Suzanne
Orfao, Alberto
Dongen, J. J. M. van
Díez, Paula
Teodosio, Cristina
author Pan, Kyra van der
author_facet Pan, Kyra van der
Khatri, Indu
Jager, Anniek L. de
Louis, Alesha
Kassem, Sara
Naber, Brigitta A. E.
Laat, Inge F. de
Hameetman, Marjolijn
Comans, Suzanne
Orfao, Alberto
Dongen, J. J. M. van
Díez, Paula
Teodosio, Cristina
author_role author
author2 Khatri, Indu
Jager, Anniek L. de
Louis, Alesha
Kassem, Sara
Naber, Brigitta A. E.
Laat, Inge F. de
Hameetman, Marjolijn
Comans, Suzanne
Orfao, Alberto
Dongen, J. J. M. van
Díez, Paula
Teodosio, Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
topic Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
description [Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/360921
url http://hdl.handle.net/10261/360921
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina. Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations. https://doi.org/10.3389/fimmu.2023.1191992 . http://hdl.handle.net/10261/348256
https://doi.org/10.3389/fimmu.2023.1191992.s002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv image/tiff
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418588678389760
score 15,81155

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