Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity

As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad...

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Autores: Ran, Zhaoshou, Xu, Jilin, Liao, Kai, Monroig, Óscar, Navarro, Juan Carlos, Oboh, Angela, Jin, Min, Zhou, Qi-Cun, Zhou, Chengxu, Tocher, Douglas R., Yan, Xiaojun
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/186455
Acceso en línea:http://hdl.handle.net/10261/186455
Access Level:acceso abierto
Palabra clave:Docosahexaenoic acid biosynthesis
Fatty acyl desaturases
Elongation of very long-chain fatty acids protein
Long-chain polyunsaturated fatty acids
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spelling Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacityRan, ZhaoshouXu, JilinLiao, KaiMonroig, ÓscarNavarro, Juan CarlosOboh, AngelaJin, MinZhou, Qi-CunZhou, ChengxuTocher, Douglas R.Yan, XiaojunDocosahexaenoic acid biosynthesisFatty acyl desaturasesElongation of very long-chain fatty acids proteinLong-chain polyunsaturated fatty acidsAs an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Here, we further successfully characterize elongases of very long-chain fatty acids (Elovl) in this important bivalve species, including one Elovl2/5, two Elovl4 isoforms (a and b) and a novel Elovl (c) with Elovl4 activity. In addition, we also determined the desaturation activity of S. constricta Δ6 Fad toward 24:5n-3 to give 24:6n-3, a key intermediate in docosahexaenoic acid (DHA) biosynthesis. Therefore, S. constricta is the first marine mollusc reported to possess all Fad and Elovl activities required for LC-PUFA biosynthesis via the ‘Sprecher pathway’. This finding greatly increases our understanding of LC-PUFA biosynthesis in marine molluscs. Phylogenetic analysis by interrogating six marine molluscan genomes, and previously functionally characterized Elovl and Fad from marine molluscs, suggested that DHA biosynthetic ability was limited to a few species, due to the general lack of Δ4 or Δ6 Fad in most molluscs.This research was supported by the National Key Research and Development Program of China (2018YFD0900702), Ningbo Science and Technology Research Projects, China (2017C110003), Zhejiang Major Science Project, China (2018C02G2201013), the Earmarked Fund for Modern Agro-industry Technology Research System, China (CARS-49), and was partly sponsored by K. C. Wong Magna Fund in Ningbo UniversityPeer reviewedElsevierNational Basic Research Program (China)Monroig, Óscar [0000-0001-8712-0440]Navarro, Juan Carlos [0000-0001-6976-6686]Tocher, Douglas R. [0000-0002-8603-9410]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/186455reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.bbalip.2019.04.004Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1864552026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
title Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
spellingShingle Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
Ran, Zhaoshou
Docosahexaenoic acid biosynthesis
Fatty acyl desaturases
Elongation of very long-chain fatty acids protein
Long-chain polyunsaturated fatty acids
title_short Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
title_full Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
title_fullStr Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
title_full_unstemmed Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
title_sort Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
dc.creator.none.fl_str_mv Ran, Zhaoshou
Xu, Jilin
Liao, Kai
Monroig, Óscar
Navarro, Juan Carlos
Oboh, Angela
Jin, Min
Zhou, Qi-Cun
Zhou, Chengxu
Tocher, Douglas R.
Yan, Xiaojun
author Ran, Zhaoshou
author_facet Ran, Zhaoshou
Xu, Jilin
Liao, Kai
Monroig, Óscar
Navarro, Juan Carlos
Oboh, Angela
Jin, Min
Zhou, Qi-Cun
Zhou, Chengxu
Tocher, Douglas R.
Yan, Xiaojun
author_role author
author2 Xu, Jilin
Liao, Kai
Monroig, Óscar
Navarro, Juan Carlos
Oboh, Angela
Jin, Min
Zhou, Qi-Cun
Zhou, Chengxu
Tocher, Douglas R.
Yan, Xiaojun
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Basic Research Program (China)
Monroig, Óscar [0000-0001-8712-0440]
Navarro, Juan Carlos [0000-0001-6976-6686]
Tocher, Douglas R. [0000-0002-8603-9410]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Docosahexaenoic acid biosynthesis
Fatty acyl desaturases
Elongation of very long-chain fatty acids protein
Long-chain polyunsaturated fatty acids
topic Docosahexaenoic acid biosynthesis
Fatty acyl desaturases
Elongation of very long-chain fatty acids protein
Long-chain polyunsaturated fatty acids
description As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Here, we further successfully characterize elongases of very long-chain fatty acids (Elovl) in this important bivalve species, including one Elovl2/5, two Elovl4 isoforms (a and b) and a novel Elovl (c) with Elovl4 activity. In addition, we also determined the desaturation activity of S. constricta Δ6 Fad toward 24:5n-3 to give 24:6n-3, a key intermediate in docosahexaenoic acid (DHA) biosynthesis. Therefore, S. constricta is the first marine mollusc reported to possess all Fad and Elovl activities required for LC-PUFA biosynthesis via the ‘Sprecher pathway’. This finding greatly increases our understanding of LC-PUFA biosynthesis in marine molluscs. Phylogenetic analysis by interrogating six marine molluscan genomes, and previously functionally characterized Elovl and Fad from marine molluscs, suggested that DHA biosynthetic ability was limited to a few species, due to the general lack of Δ4 or Δ6 Fad in most molluscs.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/186455
url http://hdl.handle.net/10261/186455
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.bbalip.2019.04.004
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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