Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)

A mapping F2 population from the cross ‘Piel de Sapo’6PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shap...

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Autores: DÍaz, Aurora, Zarouri, Belkacem, Fergany, Mohamed, Eduardo, Iban, Álvarez, José M., Picó, Belén, Monforte, Antonio J.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/3239
Acceso en línea:http://hdl.handle.net/20.500.12327/3239
https://doi.org/10.1371/journal.pone.0104188
Access Level:acceso abierto
Palabra clave:575
633
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spelling Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)DÍaz, AuroraZarouri, BelkacemFergany, MohamedEduardo, IbanÁlvarez, José M.Picó, BelénMonforte, Antonio J.575633A mapping F2 population from the cross ‘Piel de Sapo’6PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in ‘Piel de Sapo’ background which yields round melons.info:eu-repo/semantics/publishedVersionPublic Library of ScienceProducció VegetalGenòmica i Biotecnologia2014info:eu-repo/semantics/article12http://hdl.handle.net/20.500.12327/3239https://doi.org/10.1371/journal.pone.0104188reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS ONEMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-12698-C02-02/ES/El Control Genetico De La Forma Del Fruto Y Domesticacion Del Melon En Varias Fuentes De Germoplasma/MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-40130-C02-02/ES/DESCIFRANDO LA BASE GENETICA DE LA MORFOLOGIA DEL FRUTO Y LA DOMESTICACION DE MELON/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/32392026-05-29T05:05:01Z
dc.title.none.fl_str_mv Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
title Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
spellingShingle Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
DÍaz, Aurora
575
633
title_short Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
title_full Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
title_fullStr Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
title_full_unstemmed Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
title_sort Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
dc.creator.none.fl_str_mv DÍaz, Aurora
Zarouri, Belkacem
Fergany, Mohamed
Eduardo, Iban
Álvarez, José M.
Picó, Belén
Monforte, Antonio J.
author DÍaz, Aurora
author_facet DÍaz, Aurora
Zarouri, Belkacem
Fergany, Mohamed
Eduardo, Iban
Álvarez, José M.
Picó, Belén
Monforte, Antonio J.
author_role author
author2 Zarouri, Belkacem
Fergany, Mohamed
Eduardo, Iban
Álvarez, José M.
Picó, Belén
Monforte, Antonio J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Vegetal
Genòmica i Biotecnologia
dc.subject.none.fl_str_mv 575
633
topic 575
633
description A mapping F2 population from the cross ‘Piel de Sapo’6PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in ‘Piel de Sapo’ background which yields round melons.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/3239
https://doi.org/10.1371/journal.pone.0104188
url http://hdl.handle.net/20.500.12327/3239
https://doi.org/10.1371/journal.pone.0104188
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-12698-C02-02/ES/El Control Genetico De La Forma Del Fruto Y Domesticacion Del Melon En Varias Fuentes De Germoplasma/
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-40130-C02-02/ES/DESCIFRANDO LA BASE GENETICA DE LA MORFOLOGIA DEL FRUTO Y LA DOMESTICACION DE MELON/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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