Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study

Surveillance in wild birds is essential for the timely detection of high pathogenicity avian influenza (HPAI) strains. As flocks congregate in large numbers in wetlands and may potentially contaminate the environment with pathogens, the monitoring of such water bodies represents an attractive opport...

Descripción completa

Detalles Bibliográficos
Autores: Mulatti, Paolo, Terregino, Calogero, Panzarin, Valentina, Crimaudo, Marika, Bonfante, Francesco, Marciano, Sabrina, Berto, Paola, Bofill Mas, Silvia, Rusiñol Arantegui, Marta, Mazzetto, Eva, Bortolami, Alessio, Fornasiero, Diletta, Martelli, Luca
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/226747
Acceso en línea:https://hdl.handle.net/2445/226747
Access Level:acceso abierto
Palabra clave:Grip aviària
Seguiment ambiental
Zones humides
Avian influenza
Environmental monitoring
Wetlands
id ES_c19b237523f65d4064ed7b49484a6484
oai_identifier_str oai:diposit.ub.edu:2445/226747
network_acronym_str ES
network_name_str España
repository_id_str
spelling Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation StudyMulatti, PaoloTerregino, CalogeroPanzarin, ValentinaCrimaudo, MarikaBonfante, FrancescoMarciano, SabrinaBerto, PaolaBofill Mas, SilviaRusiñol Arantegui, MartaMazzetto, EvaBortolami, AlessioFornasiero, DilettaMartelli, LucaGrip aviàriaSeguiment ambientalZones humidesAvian influenzaEnvironmental monitoringWetlandsSurveillance in wild birds is essential for the timely detection of high pathogenicity avian influenza (HPAI) strains. As flocks congregate in large numbers in wetlands and may potentially contaminate the environment with pathogens, the monitoring of such water bodies represents an attractive opportunity to complement animal testing and to improve surveillance for avian influenza. To increase sensitivity, water concentration is often required but available methods based on (ultra)filtration and precipitation are mostly limited by the use of pumping equipment and by the need to identify the representative sample volumes. In contrast, passive samplers (PS) offer a cost-effective and scalable solution that requires basic devices for the deployment of adsorbent materials and minimal training for their installation in the field. This study evaluated nine materials for their virus adsorption efficiency in brackish and freshwater. Cotton gauze, nitrocellulose, and nylon showed the best performance across different deployment times, with the highest recovery after 24 h. Shorter (3 h) and longer (7 days) deployments also proved effective, accommodating different sampling regimens according to the logistical needs. Importantly, PS revealed their efficacy in adsorbing also deteriorated virions or in dynamic ecosystems subjected to changes in water volumes. Field trials in wetlands corroborated laboratory findings and demonstrated that PS allowed detecting avian influenza virus (AIV, including HPAI strains) genome in water bodies, yielding consistent results with active surveillance in wild birds. By offering a simple, cost-effective, and versatile solution, PS represent a promising tool for environmental AI monitoring and can successfully complement existing avian influenza surveillance activities.Springer Verlag2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/226747Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1007/s12560-025-09649-zFood And Environmental Virology, 2025, vol. 17, num.3, p. 1-19https://doi.org/10.1007/s12560-025-09649-zcc-by-nc-nd (c) Mulatti, Paolo et al., 2025http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2267472026-05-27T06:46:51Z
dc.title.none.fl_str_mv Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
title Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
spellingShingle Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
Mulatti, Paolo
Grip aviària
Seguiment ambiental
Zones humides
Avian influenza
Environmental monitoring
Wetlands
title_short Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
title_full Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
title_fullStr Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
title_full_unstemmed Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
title_sort Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study
dc.creator.none.fl_str_mv Mulatti, Paolo
Terregino, Calogero
Panzarin, Valentina
Crimaudo, Marika
Bonfante, Francesco
Marciano, Sabrina
Berto, Paola
Bofill Mas, Silvia
Rusiñol Arantegui, Marta
Mazzetto, Eva
Bortolami, Alessio
Fornasiero, Diletta
Martelli, Luca
author Mulatti, Paolo
author_facet Mulatti, Paolo
Terregino, Calogero
Panzarin, Valentina
Crimaudo, Marika
Bonfante, Francesco
Marciano, Sabrina
Berto, Paola
Bofill Mas, Silvia
Rusiñol Arantegui, Marta
Mazzetto, Eva
Bortolami, Alessio
Fornasiero, Diletta
Martelli, Luca
author_role author
author2 Terregino, Calogero
Panzarin, Valentina
Crimaudo, Marika
Bonfante, Francesco
Marciano, Sabrina
Berto, Paola
Bofill Mas, Silvia
Rusiñol Arantegui, Marta
Mazzetto, Eva
Bortolami, Alessio
Fornasiero, Diletta
Martelli, Luca
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Grip aviària
Seguiment ambiental
Zones humides
Avian influenza
Environmental monitoring
Wetlands
topic Grip aviària
Seguiment ambiental
Zones humides
Avian influenza
Environmental monitoring
Wetlands
description Surveillance in wild birds is essential for the timely detection of high pathogenicity avian influenza (HPAI) strains. As flocks congregate in large numbers in wetlands and may potentially contaminate the environment with pathogens, the monitoring of such water bodies represents an attractive opportunity to complement animal testing and to improve surveillance for avian influenza. To increase sensitivity, water concentration is often required but available methods based on (ultra)filtration and precipitation are mostly limited by the use of pumping equipment and by the need to identify the representative sample volumes. In contrast, passive samplers (PS) offer a cost-effective and scalable solution that requires basic devices for the deployment of adsorbent materials and minimal training for their installation in the field. This study evaluated nine materials for their virus adsorption efficiency in brackish and freshwater. Cotton gauze, nitrocellulose, and nylon showed the best performance across different deployment times, with the highest recovery after 24 h. Shorter (3 h) and longer (7 days) deployments also proved effective, accommodating different sampling regimens according to the logistical needs. Importantly, PS revealed their efficacy in adsorbing also deteriorated virions or in dynamic ecosystems subjected to changes in water volumes. Field trials in wetlands corroborated laboratory findings and demonstrated that PS allowed detecting avian influenza virus (AIV, including HPAI strains) genome in water bodies, yielding consistent results with active surveillance in wild birds. By offering a simple, cost-effective, and versatile solution, PS represent a promising tool for environmental AI monitoring and can successfully complement existing avian influenza surveillance activities.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/226747
url https://hdl.handle.net/2445/226747
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1007/s12560-025-09649-z
Food And Environmental Virology, 2025, vol. 17, num.3, p. 1-19
https://doi.org/10.1007/s12560-025-09649-z
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Mulatti, Paolo et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Mulatti, Paolo et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418573746667520
score 15.812429

Similares en LA Referencia