Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus
Lager-style beers constitute the vast majority of the beer market, and yet, the genetic origin of the yeast strains that brew them has been shrouded in mystery and controversy. Unlike ale-style beers, which are generally brewed with Saccharomyces cerevisiae, lagers are brewed at colder temperatures...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/378165 |
| Acceso en línea: | http://hdl.handle.net/10261/378165 https://api.elsevier.com/content/abstract/scopus_id/84982847704 |
| Access Level: | acceso abierto |
| Palabra clave: | Yeasts Saccharomyces eubayanus Lager brewing Genetic variation Phylogenetics |
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| dc.title.none.fl_str_mv |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| title |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| spellingShingle |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus Peris Navarro, David Yeasts Saccharomyces eubayanus Lager brewing Genetic variation Phylogenetics |
| title_short |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| title_full |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| title_fullStr |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| title_full_unstemmed |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| title_sort |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanus |
| dc.creator.none.fl_str_mv |
Peris Navarro, David Langdon, Quinn K. Moriarty, Ryan V. Sylvester, Kayla Bontrager, Martin Charron, Guillaume Leducq, Jean-Baptiste Landry, Christian R. Libkind, Diego Hittinger, Chris Todd |
| author |
Peris Navarro, David |
| author_facet |
Peris Navarro, David Langdon, Quinn K. Moriarty, Ryan V. Sylvester, Kayla Bontrager, Martin Charron, Guillaume Leducq, Jean-Baptiste Landry, Christian R. Libkind, Diego Hittinger, Chris Todd |
| author_role |
author |
| author2 |
Langdon, Quinn K. Moriarty, Ryan V. Sylvester, Kayla Bontrager, Martin Charron, Guillaume Leducq, Jean-Baptiste Landry, Christian R. Libkind, Diego Hittinger, Chris Todd |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
National Science Foundation (US) Natural Sciences and Engineering Research Council of Canada Agencia Nacional de Promoción Científica y Tecnológica (Argentina) Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) National Institute of Food and Agriculture (US) Great Lakes Bioenergy Research Center (US) Peris Navarro, David [0000-0001-9912-8802] Moriarty, Ryan V. [0000-0001-7100-1906] Bontrager, Martin [0000-0003-3013-6155] |
| dc.subject.none.fl_str_mv |
Yeasts Saccharomyces eubayanus Lager brewing Genetic variation Phylogenetics |
| topic |
Yeasts Saccharomyces eubayanus Lager brewing Genetic variation Phylogenetics |
| description |
Lager-style beers constitute the vast majority of the beer market, and yet, the genetic origin of the yeast strains that brew them has been shrouded in mystery and controversy. Unlike ale-style beers, which are generally brewed with Saccharomyces cerevisiae, lagers are brewed at colder temperatures with allopolyploid hybrids of Saccharomyces eubayanus x S. cerevisiae. Since the discovery of S. eubayanus in 2011, additional strains have been isolated from South America, North America, Australasia, and Asia, but only interspecies hybrids have been isolated in Europe. Here, using genome sequence data, we examine the relationships of these wild S. eubayanus strains to each other and to domesticated lager strains. Our results support the existence of a relatively low-diversity (π = 0.00197) lineage of S. eubayanus whose distribution stretches across the Holarctic ecozone and includes wild isolates from Tibet, new wild isolates from North America, and the S. eubayanus parents of lager yeasts. This Holarctic lineage is closely related to a population with higher diversity (π = 0.00275) that has been found primarily in South America but includes some widely distributed isolates. A second diverse South American population (π = 0.00354) and two early-diverging Asian subspecies are more distantly related. We further show that no single wild strain from the Holarctic lineage is the sole closest relative of lager yeasts. Instead, different parts of the genome portray different phylogenetic signals and ancestry, likely due to outcrossing and incomplete lineage sorting. Indeed, standing genetic variation within this wild Holarctic lineage of S. eubayanus is responsible for genetic variation still segregating among modern lager-brewing hybrids. We conclude that the relationships among wild strains of S. eubayanus and their domesticated hybrids reflect complex biogeographical and genetic processes. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/378165 https://api.elsevier.com/content/abstract/scopus_id/84982847704 |
| url |
http://hdl.handle.net/10261/378165 https://api.elsevier.com/content/abstract/scopus_id/84982847704 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
PLoS genetics The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1371/journal.pgen.1006155 https://doi.org/10.1371/journal.pgen.1006155 No |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Public Library of Science |
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Public Library of Science |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869418571628544000 |
| spelling |
Complex Ancestries of Lager-Brewing Hybrids Were Shaped by Standing Variation in the Wild Yeast Saccharomyces eubayanusPeris Navarro, DavidLangdon, Quinn K.Moriarty, Ryan V.Sylvester, KaylaBontrager, MartinCharron, GuillaumeLeducq, Jean-BaptisteLandry, Christian R.Libkind, DiegoHittinger, Chris ToddYeastsSaccharomyces eubayanusLager brewingGenetic variationPhylogeneticsLager-style beers constitute the vast majority of the beer market, and yet, the genetic origin of the yeast strains that brew them has been shrouded in mystery and controversy. Unlike ale-style beers, which are generally brewed with Saccharomyces cerevisiae, lagers are brewed at colder temperatures with allopolyploid hybrids of Saccharomyces eubayanus x S. cerevisiae. Since the discovery of S. eubayanus in 2011, additional strains have been isolated from South America, North America, Australasia, and Asia, but only interspecies hybrids have been isolated in Europe. Here, using genome sequence data, we examine the relationships of these wild S. eubayanus strains to each other and to domesticated lager strains. Our results support the existence of a relatively low-diversity (π = 0.00197) lineage of S. eubayanus whose distribution stretches across the Holarctic ecozone and includes wild isolates from Tibet, new wild isolates from North America, and the S. eubayanus parents of lager yeasts. This Holarctic lineage is closely related to a population with higher diversity (π = 0.00275) that has been found primarily in South America but includes some widely distributed isolates. A second diverse South American population (π = 0.00354) and two early-diverging Asian subspecies are more distantly related. We further show that no single wild strain from the Holarctic lineage is the sole closest relative of lager yeasts. Instead, different parts of the genome portray different phylogenetic signals and ancestry, likely due to outcrossing and incomplete lineage sorting. Indeed, standing genetic variation within this wild Holarctic lineage of S. eubayanus is responsible for genetic variation still segregating among modern lager-brewing hybrids. We conclude that the relationships among wild strains of S. eubayanus and their domesticated hybrids reflect complex biogeographical and genetic processes.This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1256259 to QKL and MB; QKL and MB were also supported by the Predoctoral Training Program in Genetics, funded by the National Institutes of Health (5 T32 GM007133-40). CRL was funded by a Discovery grant from the Natural Sciences and Engineering Research Council of Canada (NSERC). CRL holds the Canada Research Chair in Evolutionary Cell and Systems Biology. DL was funded by ANPCyT (PICT2011-1814, and PICT2014-2542), UNComahue (B171), CONICET (11220130100392CO), and NSF-CONICET Bilateral Cooperation Projects. This material is based upon work supported by the National Science Foundation under Grant No. DEB-1253634 to CTH, by USDA National Institute of Food and Agriculture Hatch Project 1003258, and funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494).Peer reviewedPublic Library of ScienceNational Science Foundation (US)Natural Sciences and Engineering Research Council of CanadaAgencia Nacional de Promoción Científica y Tecnológica (Argentina)Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)National Institute of Food and Agriculture (US)Great Lakes Bioenergy Research Center (US)Peris Navarro, David [0000-0001-9912-8802]Moriarty, Ryan V. [0000-0001-7100-1906]Bontrager, Martin [0000-0003-3013-6155]202520252016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/378165https://api.elsevier.com/content/abstract/scopus_id/84982847704reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPLoS geneticsThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1371/journal.pgen.1006155https://doi.org/10.1371/journal.pgen.1006155Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3781652026-05-22T06:33:51Z |
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15,811543 |