RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage

Cytopathic viruses have developed successful strategies to block or, at least, to attenuate host interference with their replication. Here, we have analyzed the effects of poliovirus 2A protease on RNA nuclear export. 2A protease interferes with trafficking of mRNAs, rRNAs and U snRNAs from the nucl...

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Detalles Bibliográficos
Autores: Castelló, Alfredo, Izquierdo, José M., Welnowska, Ewelina, Carrasco Llamas, Luis
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24243
Acceso en línea:http://hdl.handle.net/10261/24243
Access Level:acceso abierto
Palabra clave:2A protease
RNA nuclear export
Nucleoporin
Interferon
Gene Expression Control
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spelling RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavageCastelló, AlfredoIzquierdo, José M.Welnowska, EwelinaCarrasco Llamas, Luis2A proteaseRNA nuclear exportNucleoporinInterferonGene Expression ControlCytopathic viruses have developed successful strategies to block or, at least, to attenuate host interference with their replication. Here, we have analyzed the effects of poliovirus 2A protease on RNA nuclear export. 2A protease interferes with trafficking of mRNAs, rRNAs and U snRNAs from the nucleus to the cytoplasm, without any apparent effect on tRNA transport. Traffic of newly produced mRNAs is more strongly affected than traffic of other mRNAs over-represented in the cytoplasm, such as mRNA encoding -actin. Inhibition of RNA nuclear export in HeLa cells expressing 2A protease is concomitant with the cleavage of Nup98, Nup153, Nup62 and their subsequent subcellular redistribution. The expression of an inactive 2A protease failed to interfere with RNA nuclear export. In addition, other related proteases, such as poliovirus 3C or foot and mouth disease virus Lpro did not affect mRNA distribution or Nup98 integrity. Treatment of HeLa cells with interferon (IFN)- increased the relative amount of Nup98. Under such conditions, the cleavage of Nup98 induced by 2A protease is partial, and thus IFN- prevents the inhibition of RNA nuclear export. Taken together, these results are consistent with a specific proteolysis of Nup98 by 2A protease to prevent de novo mRNA traffic in poliovirus-infected cells.Peer reviewedCompany of Biologists201020102009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65012413332 bytesapplication/pdfhttp://hdl.handle.net/10261/24243reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/doi:10.1242/jcs.055988info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/242432026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
title RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
spellingShingle RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
Castelló, Alfredo
2A protease
RNA nuclear export
Nucleoporin
Interferon
Gene Expression Control
title_short RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
title_full RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
title_fullStr RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
title_full_unstemmed RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
title_sort RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
dc.creator.none.fl_str_mv Castelló, Alfredo
Izquierdo, José M.
Welnowska, Ewelina
Carrasco Llamas, Luis
author Castelló, Alfredo
author_facet Castelló, Alfredo
Izquierdo, José M.
Welnowska, Ewelina
Carrasco Llamas, Luis
author_role author
author2 Izquierdo, José M.
Welnowska, Ewelina
Carrasco Llamas, Luis
author2_role author
author
author
dc.subject.none.fl_str_mv 2A protease
RNA nuclear export
Nucleoporin
Interferon
Gene Expression Control
topic 2A protease
RNA nuclear export
Nucleoporin
Interferon
Gene Expression Control
description Cytopathic viruses have developed successful strategies to block or, at least, to attenuate host interference with their replication. Here, we have analyzed the effects of poliovirus 2A protease on RNA nuclear export. 2A protease interferes with trafficking of mRNAs, rRNAs and U snRNAs from the nucleus to the cytoplasm, without any apparent effect on tRNA transport. Traffic of newly produced mRNAs is more strongly affected than traffic of other mRNAs over-represented in the cytoplasm, such as mRNA encoding -actin. Inhibition of RNA nuclear export in HeLa cells expressing 2A protease is concomitant with the cleavage of Nup98, Nup153, Nup62 and their subsequent subcellular redistribution. The expression of an inactive 2A protease failed to interfere with RNA nuclear export. In addition, other related proteases, such as poliovirus 3C or foot and mouth disease virus Lpro did not affect mRNA distribution or Nup98 integrity. Treatment of HeLa cells with interferon (IFN)- increased the relative amount of Nup98. Under such conditions, the cleavage of Nup98 induced by 2A protease is partial, and thus IFN- prevents the inhibition of RNA nuclear export. Taken together, these results are consistent with a specific proteolysis of Nup98 by 2A protease to prevent de novo mRNA traffic in poliovirus-infected cells.
publishDate 2009
dc.date.none.fl_str_mv 2009
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24243
url http://hdl.handle.net/10261/24243
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/doi:10.1242/jcs.055988
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2413332 bytes
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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