Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles

Background: Morin flavonoid exerts neuroprotective effects with potential interest in neurodegenerative disorders. For this, the use of surface-modified polymeric nanoparticles loaded with morin is an interesting approach. Objective: To develop and validate an HPLC method for the quantification of m...

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Autores: Alonso González, Mario, Barcia Hernández, Emilia María, Córdoba Díaz, Manuel, Negro Álvarez, María Sofía Elisa, Córdoba Díaz, Damián, Fernández Carballido, Ana María
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/115519
Acceso en línea:https://hdl.handle.net/20.500.14352/115519
Access Level:acceso abierto
Palabra clave:615.4
HPLC
morin
PLGA
flavonoids
nanoparticles
phenilalanine
Tecnología farmaceútica
3209.01 Análisis de Medicamentos
3209.08 Preparación de Medicamentos
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oai_identifier_str oai:docta.ucm.es:20.500.14352/115519
network_acronym_str ES
network_name_str España
repository_id_str
spelling Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA NanoparticlesAlonso González, MarioBarcia Hernández, Emilia MaríaCórdoba Díaz, ManuelNegro Álvarez, María Sofía ElisaCórdoba Díaz, DamiánFernández Carballido, Ana María615.4HPLCmorinPLGAflavonoidsnanoparticlesphenilalanineTecnología farmaceútica3209.01 Análisis de Medicamentos3209.08 Preparación de MedicamentosBackground: Morin flavonoid exerts neuroprotective effects with potential interest in neurodegenerative disorders. For this, the use of surface-modified polymeric nanoparticles loaded with morin is an interesting approach. Objective: To develop and validate an HPLC method for the quantification of morin released from a new delivery system consisting of poly lactic-co-glycolic (PLGA) nanoparticles functionalized with the dipeptide phe-phe (phenylalanine-phenylalanine) used to facilitate their access to the CNS. Methods: The HPLC procedure was developed and validated with morin hydrate dissolved either in methanol (method A) or in methanol: 0.1N HCl (50:50, v/v, method B). Two new nanoparticle formulations were developed and characterized: morin-loaded PLGA nanoparticles (formulation F1), and morin-loaded PLGA phe-phe nanoparticles (formulation F2). Results: Method A was linear within the concentration range of 5-30 μg.mL-1 and, 1-30 μg mL-1 for method B. LOD and LOQ with method A were 1.23 μg.mL-1 and 3.90 μg.mL-1, respectively, and 0.481 μg.mL-1 and 1.458 μg.mL-1, respectively for method B. The average amount of phe-phe bound to formulation F2 (50 mg of NPs) was 431.33 μg. The encapsulation efficiency of morin within PLGA nanoparticles was around 80%. After functionalization, this value decreased significantly. Conclusion: Method B showed better sensitivity, accuracy, and precision for the quantification of morin. The procedure used to functionalize the nanoparticles was adequate for linking the dipeptide to their surfaces, but this procedure is not adequate when encapsulating water-soluble compounds.Bentham Science PublishersUniversidad Complutense de Madrid20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/115519reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1155192026-06-02T12:44:21Z
dc.title.none.fl_str_mv Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
title Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
spellingShingle Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
Alonso González, Mario
615.4
HPLC
morin
PLGA
flavonoids
nanoparticles
phenilalanine
Tecnología farmaceútica
3209.01 Análisis de Medicamentos
3209.08 Preparación de Medicamentos
title_short Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
title_full Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
title_fullStr Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
title_full_unstemmed Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
title_sort Development and Validation of an HPLC Method for the Quantification of Morin Flavonoid Encapsulated within PLGA Nanoparticles
dc.creator.none.fl_str_mv Alonso González, Mario
Barcia Hernández, Emilia María
Córdoba Díaz, Manuel
Negro Álvarez, María Sofía Elisa
Córdoba Díaz, Damián
Fernández Carballido, Ana María
author Alonso González, Mario
author_facet Alonso González, Mario
Barcia Hernández, Emilia María
Córdoba Díaz, Manuel
Negro Álvarez, María Sofía Elisa
Córdoba Díaz, Damián
Fernández Carballido, Ana María
author_role author
author2 Barcia Hernández, Emilia María
Córdoba Díaz, Manuel
Negro Álvarez, María Sofía Elisa
Córdoba Díaz, Damián
Fernández Carballido, Ana María
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.4
HPLC
morin
PLGA
flavonoids
nanoparticles
phenilalanine
Tecnología farmaceútica
3209.01 Análisis de Medicamentos
3209.08 Preparación de Medicamentos
topic 615.4
HPLC
morin
PLGA
flavonoids
nanoparticles
phenilalanine
Tecnología farmaceútica
3209.01 Análisis de Medicamentos
3209.08 Preparación de Medicamentos
description Background: Morin flavonoid exerts neuroprotective effects with potential interest in neurodegenerative disorders. For this, the use of surface-modified polymeric nanoparticles loaded with morin is an interesting approach. Objective: To develop and validate an HPLC method for the quantification of morin released from a new delivery system consisting of poly lactic-co-glycolic (PLGA) nanoparticles functionalized with the dipeptide phe-phe (phenylalanine-phenylalanine) used to facilitate their access to the CNS. Methods: The HPLC procedure was developed and validated with morin hydrate dissolved either in methanol (method A) or in methanol: 0.1N HCl (50:50, v/v, method B). Two new nanoparticle formulations were developed and characterized: morin-loaded PLGA nanoparticles (formulation F1), and morin-loaded PLGA phe-phe nanoparticles (formulation F2). Results: Method A was linear within the concentration range of 5-30 μg.mL-1 and, 1-30 μg mL-1 for method B. LOD and LOQ with method A were 1.23 μg.mL-1 and 3.90 μg.mL-1, respectively, and 0.481 μg.mL-1 and 1.458 μg.mL-1, respectively for method B. The average amount of phe-phe bound to formulation F2 (50 mg of NPs) was 431.33 μg. The encapsulation efficiency of morin within PLGA nanoparticles was around 80%. After functionalization, this value decreased significantly. Conclusion: Method B showed better sensitivity, accuracy, and precision for the quantification of morin. The procedure used to functionalize the nanoparticles was adequate for linking the dipeptide to their surfaces, but this procedure is not adequate when encapsulating water-soluble compounds.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/115519
url https://hdl.handle.net/20.500.14352/115519
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418523159166976
score 15,81155

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