Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA

[EN] Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the potential of these ribozymes for targeting longer and structured RNAs in vitro a...

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Autores: CARBONELL, ALBERTO|||0000-0001-5628-6632, Flores Pedauye, Ricardo, Gago Zachert, Selma Persida
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/28303
Acceso en línea:https://riunet.upv.es/handle/10251/28303
Access Level:acceso abierto
Palabra clave:Hammerhead
Viroid
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spelling Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNACARBONELL, ALBERTO|||0000-0001-5628-6632Flores Pedauye, RicardoGago Zachert, Selma PersidaHammerheadViroid[EN] Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the potential of these ribozymes for targeting longer and structured RNAs in vitro and in vivo has not been examined. Here, we report the in vitro cleavage of short RNAs and of a 464-nt highly structured RNA from potato spindle tuber viroid (PSTVd) by hammerheads with discontinuous and extended formats at submillimolar Mg2+. Under these conditions, hammerheads derived from eggplant latent viroid and peach latent mosaic viroid (PLMVd) with discontinuous and extended formats, respectively, where the most active. Furthermore, a PLMVd-derived hammerhead with natural TSMs showed activity in vivo against the same long substrate and interfered with systemic PSTVd infection, thus reinforcing the idea that this class of ribozymes has potential to control pathogenic RNA replicons.Ministerio de Ciencia e Innovacion (BFU2008-03154/BMC to R.F.); Generalidad Valenciana (ACOMP/2010/278 to R.F.); Ministerio de Educacion y Ciencia (Predoctoral fellowship to A.C.); and Consejo Superior de Investigaciones Cientificas (Postdoctoral I3P contract to S.G.) of Spain. Funding for open access charge: Ministerio de Ciencia e Innovacion (BFU2008-03154/BMC to R.F.); Generalidad Valenciana (ACOMP/2010/278 to R.F.).Oxford University Press (OUP): Policy C - Option BInstituto Universitario Mixto de Biología Molecular y Celular de PlantasMinisterio de Ciencia e InnovaciónGeneralitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20112011-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/28303reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2008-03154 INTERACCIONES VIROIDE-HUESPED: PAPEL DE LAS RIBOZIMAS, DEL SILENCIAMIENTO MEDIADO POR RNA, Y DE LA RECOMBINACION DE RNAGeneralitat Valenciana https://doi.org/10.13039/501100003359 ACOMP%2F2010%2F 278open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial (by-nc) http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/283032026-06-13T07:49:27Z
dc.title.none.fl_str_mv Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
title Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
spellingShingle Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
CARBONELL, ALBERTO|||0000-0001-5628-6632
Hammerhead
Viroid
title_short Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
title_full Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
title_fullStr Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
title_full_unstemmed Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
title_sort Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA
dc.creator.none.fl_str_mv CARBONELL, ALBERTO|||0000-0001-5628-6632
Flores Pedauye, Ricardo
Gago Zachert, Selma Persida
author CARBONELL, ALBERTO|||0000-0001-5628-6632
author_facet CARBONELL, ALBERTO|||0000-0001-5628-6632
Flores Pedauye, Ricardo
Gago Zachert, Selma Persida
author_role author
author2 Flores Pedauye, Ricardo
Gago Zachert, Selma Persida
author2_role author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Ministerio de Ciencia e Innovación
Generalitat Valenciana
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Hammerhead
Viroid
topic Hammerhead
Viroid
description [EN] Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the potential of these ribozymes for targeting longer and structured RNAs in vitro and in vivo has not been examined. Here, we report the in vitro cleavage of short RNAs and of a 464-nt highly structured RNA from potato spindle tuber viroid (PSTVd) by hammerheads with discontinuous and extended formats at submillimolar Mg2+. Under these conditions, hammerheads derived from eggplant latent viroid and peach latent mosaic viroid (PLMVd) with discontinuous and extended formats, respectively, where the most active. Furthermore, a PLMVd-derived hammerhead with natural TSMs showed activity in vivo against the same long substrate and interfered with systemic PSTVd infection, thus reinforcing the idea that this class of ribozymes has potential to control pathogenic RNA replicons.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/28303
url https://riunet.upv.es/handle/10251/28303
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2008-03154 INTERACCIONES VIROIDE-HUESPED: PAPEL DE LAS RIBOZIMAS, DEL SILENCIAMIENTO MEDIADO POR RNA, Y DE LA RECOMBINACION DE RNA
Generalitat Valenciana https://doi.org/10.13039/501100003359 ACOMP%2F2010%2F 278
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press (OUP): Policy C - Option B
publisher.none.fl_str_mv Oxford University Press (OUP): Policy C - Option B
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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