Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.

Blasco Mínguez, Josefina; Vélez, Emilio J.; Perelló-Amorós, Miquel; Azizi, Sheida; Capilla Campos, Encarnación; Fernández Borrás, J. (Jaume); Gutiérrez Fruitós, Joaquín

Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.

Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter. An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthea...

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Autores:	Blasco Mínguez, Josefina, Vélez, Emilio J., Perelló-Amorós, Miquel, Azizi, Sheida, Capilla Campos, Encarnación, Fernández Borrás, J. (Jaume), Gutiérrez Fruitós, Joaquín
Formato:	artículo
Estado:	Versión publicada
Fecha de publicación:	2021
País:	España
Recursos:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/181764
Acesso em linha:	https://hdl.handle.net/2445/181764
Access Level:	acceso abierto
Palavra-chave:	Metabolisme Citocrom c Enzims Orada Metabolism Cytochrome c Enzymes Sparus aurata

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spelling	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.Blasco Mínguez, JosefinaVélez, Emilio J.Perelló-Amorós, MiquelAzizi, SheidaCapilla Campos, EncarnaciónFernández Borrás, J. (Jaume)Gutiérrez Fruitós, JoaquínMetabolismeCitocrom cEnzimsOradaMetabolismCytochrome cEnzymesSparus aurataGrowth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter. An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses. GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation. In white and red muscle reserves, rBGH increased glycogen while reducing lipid. The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect. The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group. Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes. The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream. There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.MDPI2021202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion19 p.application/pdfhttps://hdl.handle.net/2445/181764Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/ijms222313107International Journal of Molecular Sciences, 2021, vol. 22, num. 23, p. 1-19https://doi.org/10.3390/ijms222313107cc-by (c) Blasco Mínguez, Josefina et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1817642026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
title	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
spellingShingle	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics. Blasco Mínguez, Josefina Metabolisme Citocrom c Enzims Orada Metabolism Cytochrome c Enzymes Sparus aurata
title_short	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
title_full	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
title_fullStr	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
title_full_unstemmed	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
title_sort	Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.
dc.creator.none.fl_str_mv	Blasco Mínguez, Josefina Vélez, Emilio J. Perelló-Amorós, Miquel Azizi, Sheida Capilla Campos, Encarnación Fernández Borrás, J. (Jaume) Gutiérrez Fruitós, Joaquín
author	Blasco Mínguez, Josefina
author_facet	Blasco Mínguez, Josefina Vélez, Emilio J. Perelló-Amorós, Miquel Azizi, Sheida Capilla Campos, Encarnación Fernández Borrás, J. (Jaume) Gutiérrez Fruitós, Joaquín
author_role	author
author2	Vélez, Emilio J. Perelló-Amorós, Miquel Azizi, Sheida Capilla Campos, Encarnación Fernández Borrás, J. (Jaume) Gutiérrez Fruitós, Joaquín
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Metabolisme Citocrom c Enzims Orada Metabolism Cytochrome c Enzymes Sparus aurata
topic	Metabolisme Citocrom c Enzims Orada Metabolism Cytochrome c Enzymes Sparus aurata
description	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter. An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses. GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation. In white and red muscle reserves, rBGH increased glycogen while reducing lipid. The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect. The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group. Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes. The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream. There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
publishDate	2021
dc.date.none.fl_str_mv	2021 2021 2021 2021
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/181764
url	https://hdl.handle.net/2445/181764
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.3390/ijms222313107 International Journal of Molecular Sciences, 2021, vol. 22, num. 23, p. 1-19 https://doi.org/10.3390/ijms222313107
dc.rights.none.fl_str_mv	cc-by (c) Blasco Mínguez, Josefina et al., 2021 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Blasco Mínguez, Josefina et al., 2021 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	19 p. application/pdf
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.812429

Recombinant bovine growth hormone-induced metabolic remodelling enhances growth of gilthead sea-bream (Sparus aurata): insights from stable isotopes composition and proteomics.

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