Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae
Phages and bacteria have acquired resistance mechanisms for protection. In this context, the aims of the present study were to analyze the proteins isolated from 21 novel lytic phages of Klebsiella pneumoniae in search of defense mechanisms against bacteria and also to determine the infective capaci...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/168258 |
| Acceso en línea: | https://hdl.handle.net/11441/168258 https://doi.org/10.1128/spectrum.03974-22 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacteriophage Bacteriophage evolution Defense mechanism Klebsiella Klebsiella pneumoniae Lytic phage Phage-host interaction Plasmid Prophage Virus-host interactions |
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Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella PneumoniaeBleriot, InésBlasco, LucíaPacios, OlgaFernández García, LauraLópez, MaríaOrtiz Cartagena, ConchaBarrio Pujante, AntonioFernández Cuenca, Felipe ManuelPascual Hernández, ÁlvaroMartínez Martínez, LuisOteo Iglesias, JesúsTomás, MaríaBacteriophageBacteriophage evolutionDefense mechanismKlebsiellaKlebsiella pneumoniaeLytic phagePhage-host interactionPlasmidProphageVirus-host interactionsPhages and bacteria have acquired resistance mechanisms for protection. In this context, the aims of the present study were to analyze the proteins isolated from 21 novel lytic phages of Klebsiella pneumoniae in search of defense mechanisms against bacteria and also to determine the infective capacity of the phages. A proteomic study was also conducted to investigate the defense mechanisms of two clinical isolates of K. pneumoniae infected by phages. For this purpose, the 21 lytic phages were sequenced and de novo assembled. The host range was determined in a collection of 47 clinical isolates of K. pneumoniae, revealing the variable infective capacity of the phages. Genome sequencing showed that all of the phages were lytic phages belonging to the order Caudovirales. Phage sequence analysis revealed that the proteins were organized in functional modules within the genome. Although most of the proteins have unknown functions, multiple proteins were associated with defense mechanisms against bacteria, including the restriction-modification system, the toxin-antitoxin system, evasion of DNA degradation, blocking of host restriction and modification, the orphan CRISPR-Cas system, and the anti-CRISPR system. Proteomic study of the phage-host interactions (i.e., between isolates K3574 and K3320, which have intact CRISPR-Cas systems, and phages vB_KpnS-VAC35 and vB_KpnM-VAC36, respectively) revealed the presence of several defense mechanisms against phage infection (prophage, defense/virulence/resistance, oxidative stress and plasmid proteins) in the bacteria, and of the Acr candidate (anti-CRISPR protein) in the phages.Ministerio de Ciencia, Innovación y Universidades PI19/00878, PI22/00323Instituto de Salud Carlos III RD16/0016/0006, RD16/0016/0008, FI20/00302Centro de Investigación Biomédica en Red Enfermedades Infecciosas CIBER21/13/00012, CB21/13/00049, CIBER21/13/00084, CIBER21/13/00095European Union PMP/00092Xunta de Galicia IN606A-2020/035, IN606B-2021/013, IN606C-2022/002American Society for MicrobiologyMicrobiologíaMinisterio de Ciencia, Innovación y Universidades (MICINN). EspañaInstituto de Salud Carlos IIICentro de Investigación Biomédica en Red Enfermedades Infecciosas (CIBERINFEC)European Union (UE)Xunta de Galicia2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/168258https://doi.org/10.1128/spectrum.03974-22reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMicrobiology Spectrum, 11 (2), e03974-22.PI19/00878PI22/00323RD16/0016/0006RD16/0016/0008FI20/00302CIBER21/13/00012CB21/13/00049CIBER21/13/00084CIBER21/13/00095PMP/00092IN606A-2020/035IN606B-2021/013IN606C-2022/002https://doi.org/10.1128/spectrum.03974-22info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1682582026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| title |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| spellingShingle |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae Bleriot, Inés Bacteriophage Bacteriophage evolution Defense mechanism Klebsiella Klebsiella pneumoniae Lytic phage Phage-host interaction Plasmid Prophage Virus-host interactions |
| title_short |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| title_full |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| title_fullStr |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| title_full_unstemmed |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| title_sort |
Proteomic Study of the Interactions between Phages and the Bacterial Host Klebsiella Pneumoniae |
| dc.creator.none.fl_str_mv |
Bleriot, Inés Blasco, Lucía Pacios, Olga Fernández García, Laura López, María Ortiz Cartagena, Concha Barrio Pujante, Antonio Fernández Cuenca, Felipe Manuel Pascual Hernández, Álvaro Martínez Martínez, Luis Oteo Iglesias, Jesús Tomás, María |
| author |
Bleriot, Inés |
| author_facet |
Bleriot, Inés Blasco, Lucía Pacios, Olga Fernández García, Laura López, María Ortiz Cartagena, Concha Barrio Pujante, Antonio Fernández Cuenca, Felipe Manuel Pascual Hernández, Álvaro Martínez Martínez, Luis Oteo Iglesias, Jesús Tomás, María |
| author_role |
author |
| author2 |
Blasco, Lucía Pacios, Olga Fernández García, Laura López, María Ortiz Cartagena, Concha Barrio Pujante, Antonio Fernández Cuenca, Felipe Manuel Pascual Hernández, Álvaro Martínez Martínez, Luis Oteo Iglesias, Jesús Tomás, María |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Microbiología Ministerio de Ciencia, Innovación y Universidades (MICINN). España Instituto de Salud Carlos III Centro de Investigación Biomédica en Red Enfermedades Infecciosas (CIBERINFEC) European Union (UE) Xunta de Galicia |
| dc.subject.none.fl_str_mv |
Bacteriophage Bacteriophage evolution Defense mechanism Klebsiella Klebsiella pneumoniae Lytic phage Phage-host interaction Plasmid Prophage Virus-host interactions |
| topic |
Bacteriophage Bacteriophage evolution Defense mechanism Klebsiella Klebsiella pneumoniae Lytic phage Phage-host interaction Plasmid Prophage Virus-host interactions |
| description |
Phages and bacteria have acquired resistance mechanisms for protection. In this context, the aims of the present study were to analyze the proteins isolated from 21 novel lytic phages of Klebsiella pneumoniae in search of defense mechanisms against bacteria and also to determine the infective capacity of the phages. A proteomic study was also conducted to investigate the defense mechanisms of two clinical isolates of K. pneumoniae infected by phages. For this purpose, the 21 lytic phages were sequenced and de novo assembled. The host range was determined in a collection of 47 clinical isolates of K. pneumoniae, revealing the variable infective capacity of the phages. Genome sequencing showed that all of the phages were lytic phages belonging to the order Caudovirales. Phage sequence analysis revealed that the proteins were organized in functional modules within the genome. Although most of the proteins have unknown functions, multiple proteins were associated with defense mechanisms against bacteria, including the restriction-modification system, the toxin-antitoxin system, evasion of DNA degradation, blocking of host restriction and modification, the orphan CRISPR-Cas system, and the anti-CRISPR system. Proteomic study of the phage-host interactions (i.e., between isolates K3574 and K3320, which have intact CRISPR-Cas systems, and phages vB_KpnS-VAC35 and vB_KpnM-VAC36, respectively) revealed the presence of several defense mechanisms against phage infection (prophage, defense/virulence/resistance, oxidative stress and plasmid proteins) in the bacteria, and of the Acr candidate (anti-CRISPR protein) in the phages. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/168258 https://doi.org/10.1128/spectrum.03974-22 |
| url |
https://hdl.handle.net/11441/168258 https://doi.org/10.1128/spectrum.03974-22 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Microbiology Spectrum, 11 (2), e03974-22. PI19/00878 PI22/00323 RD16/0016/0006 RD16/0016/0008 FI20/00302 CIBER21/13/00012 CB21/13/00049 CIBER21/13/00084 CIBER21/13/00095 PMP/00092 IN606A-2020/035 IN606B-2021/013 IN606C-2022/002 https://doi.org/10.1128/spectrum.03974-22 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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American Society for Microbiology |
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American Society for Microbiology |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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