Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)

We have designed a set of exon-primed intron-crossing (EPIC) PCR primers to amplify introns at the genes TBP and SRP54 in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus), and also one intron at a histone 3 homologous gene in the Manila clam. The primers we...

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Authors: Cordero, David, Peña, Juan B., Saavedra, Carlos Felipe
Format: article
Publication Date:2008
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/71637
Online Access:http://hdl.handle.net/10261/71637
Access Level:Open access
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spelling Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)Cordero, DavidPeña, Juan B.Saavedra, Carlos FelipeWe have designed a set of exon-primed intron-crossing (EPIC) PCR primers to amplify introns at the genes TBP and SRP54 in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus), and also one intron at a histone 3 homologous gene in the Manila clam. The primers were developed by using >universal> EPIC primers available in the literature and by searching for intron locations in cDNA sequences taken from public databases. The identity of the amplified products was checked by sequencing. The three introns of the Manila clam, and one in the carpet shell clam (TBP), exhibited length polymorphisms. The number of alleles was two at the TBP locus of the grooved carpet-shell clam, and ranged from three to five in the three loci of the Manila clam. The locus without length polymorphism in the carpet-shell clam exhibited polymorphism when digested with the restriction enzyme EcoR I (4 haplotypes). The variability of the markers was examined in two population samples in each species.This work has been funded by research grants AGL2003-04143 and AGL2006-08944 to J.B.P., and by the ‘‘Ramón y Cajal’’ program (C.S.), from the Spanish Ministry of Education and Science.Peer ReviewedNational Shellfisheries Association2013201320082013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/71637reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/716372026-05-22T06:33:51Z
dc.title.none.fl_str_mv Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
title Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
spellingShingle Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
Cordero, David
title_short Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
title_full Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
title_fullStr Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
title_full_unstemmed Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
title_sort Polymorphisms at three introns in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus)
dc.creator.none.fl_str_mv Cordero, David
Peña, Juan B.
Saavedra, Carlos Felipe
author Cordero, David
author_facet Cordero, David
Peña, Juan B.
Saavedra, Carlos Felipe
author_role author
author2 Peña, Juan B.
Saavedra, Carlos Felipe
author2_role author
author
description We have designed a set of exon-primed intron-crossing (EPIC) PCR primers to amplify introns at the genes TBP and SRP54 in the Manila clam (Ruditapes philippinarum) and the grooved carpet-shell clam (R. decussatus), and also one intron at a histone 3 homologous gene in the Manila clam. The primers were developed by using >universal> EPIC primers available in the literature and by searching for intron locations in cDNA sequences taken from public databases. The identity of the amplified products was checked by sequencing. The three introns of the Manila clam, and one in the carpet shell clam (TBP), exhibited length polymorphisms. The number of alleles was two at the TBP locus of the grooved carpet-shell clam, and ranged from three to five in the three loci of the Manila clam. The locus without length polymorphism in the carpet-shell clam exhibited polymorphism when digested with the restriction enzyme EcoR I (4 haplotypes). The variability of the markers was examined in two population samples in each species.
publishDate 2008
dc.date.none.fl_str_mv 2008
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/71637
url http://hdl.handle.net/10261/71637
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Shellfisheries Association
publisher.none.fl_str_mv National Shellfisheries Association
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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