Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

This study examined the effect of konjac glucomannan (KGM) supplementation in diets with different lipid levels on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in juvenile pompano (Trachinotus ovatus). Triplicate groups of fish (12.07 ± 0.21 g) were fed eight expe...

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Autores: Li, Yang, Liang, Shusheng, Shao, Yiru, Li, Yuanyou, Chen, Cuiying, You, Cuihong, Monroig, Óscar, Rahimnejad, Samad, Tocher, Douglas R., Wang, Shuqi
Tipo de documento: artigo
Estado:Versión enviada para evaluación y publicación
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/267098
Acesso em linha:http://hdl.handle.net/10261/267098
Access Level:Acceso aberto
Palavra-chave:Konjac glucomannan
High-fat diets
Antioxidant capacity
Hepatic lipid metabolism
Inflammatory response
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
title Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
spellingShingle Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
Li, Yang
Konjac glucomannan
High-fat diets
Antioxidant capacity
Hepatic lipid metabolism
Inflammatory response
title_short Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
title_full Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
title_fullStr Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
title_full_unstemmed Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
title_sort Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet
dc.creator.none.fl_str_mv Li, Yang
Liang, Shusheng
Shao, Yiru
Li, Yuanyou
Chen, Cuiying
You, Cuihong
Monroig, Óscar
Rahimnejad, Samad
Tocher, Douglas R.
Wang, Shuqi
author Li, Yang
author_facet Li, Yang
Liang, Shusheng
Shao, Yiru
Li, Yuanyou
Chen, Cuiying
You, Cuihong
Monroig, Óscar
Rahimnejad, Samad
Tocher, Douglas R.
Wang, Shuqi
author_role author
author2 Liang, Shusheng
Shao, Yiru
Li, Yuanyou
Chen, Cuiying
You, Cuihong
Monroig, Óscar
Rahimnejad, Samad
Tocher, Douglas R.
Wang, Shuqi
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv China Agricultural University
Guangdong Science and Technology Department
Natural Science Foundation of Guangdong Province
Southern Marine Science and Engineering Guangdong Laboratory
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Konjac glucomannan
High-fat diets
Antioxidant capacity
Hepatic lipid metabolism
Inflammatory response
topic Konjac glucomannan
High-fat diets
Antioxidant capacity
Hepatic lipid metabolism
Inflammatory response
description This study examined the effect of konjac glucomannan (KGM) supplementation in diets with different lipid levels on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in juvenile pompano (Trachinotus ovatus). Triplicate groups of fish (12.07 ± 0.21 g) were fed eight experimental diets containing two lipid levels of 9% (basal-fat diet, BFD) and 18% (high-fat diet, HFD), and four levels of KGM (0, 0.5, 1 and 2%) for 60 days. Pompano fed HFD exhibited markedly lower growth and feed efficiency than fish fed BFD, and KGM supplementation, particularly at 1% inclusion, significantly improved growth performance and feed efficiency in fish fed both BFD and HFD. In addition, Pompano fed HFD showed higher whole-body lipid and lower protein contents than fish fed BFD. Irrespective of dietary lipid level, whole body, dorsal and ventral muscle were reduced by dietary KGM supplementation up to 1% while the opposite was found for whole body protein content. Dietary KGM also significantly reduced the HSI, liver lipid contents, as well as lipid droplets in the liver. Supplementation of KGM to both BFD and HFD significantly reduced serum total cholesterol, triglyceride, low-density lipoprotein cholesterol and free fatty acids levels. Furthermore, irrespective of dietary lipid level, increasing dietary KGM supplementation enhanced serum IgM level, lysozyme and alkaline phosphatase activities, and reduced serum alanine aminotransferase and aspartate aminotransferase activities. Dietary KGM also increased liver total antioxidant capacity, superoxide dismutase and catalase activities, and decreased malondialdehyde concentration in fish fed both HFD and BFD. Dietary KGM significantly increased serum glutathione peroxidase, superoxide dismutase and catalase activities and reduced malondialdehyde concentrations in fish fed BFD, and enhanced serum total antioxidant capacity and glutathione peroxidase activity in fish fed HFD. Supplementation of KGM increased the expression of genes associated with lipid catabolism (pparα, cpt1, hsl) and lipid transport (fabp1, apoB100 and cd36), and decreased the expression of lipid anabolism related genes (srebp1, pparγ, fas and acc). Moreover, the expression of pro-inflammatory cytokines including tumor necrosis factor α and interleukin-8 were down-regulated, while expression of the anti-inflammatory cytokine transforming growth factor β1 was increased by KGM supplementation. In conclusion, supplementation of KGM at 1% of diet improved growth, feed efficiency, antioxidant capacity, hepatic lipid metabolism and inflammatory responses in juvenile pompano, and can mitigate excessive deposition of lipid in tissues of farmed fish.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/267098
url http://hdl.handle.net/10261/267098
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.aquaculture.2021.737113
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat dietLi, YangLiang, ShushengShao, YiruLi, YuanyouChen, CuiyingYou, CuihongMonroig, ÓscarRahimnejad, SamadTocher, Douglas R.Wang, ShuqiKonjac glucomannanHigh-fat dietsAntioxidant capacityHepatic lipid metabolismInflammatory responseThis study examined the effect of konjac glucomannan (KGM) supplementation in diets with different lipid levels on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in juvenile pompano (Trachinotus ovatus). Triplicate groups of fish (12.07 ± 0.21 g) were fed eight experimental diets containing two lipid levels of 9% (basal-fat diet, BFD) and 18% (high-fat diet, HFD), and four levels of KGM (0, 0.5, 1 and 2%) for 60 days. Pompano fed HFD exhibited markedly lower growth and feed efficiency than fish fed BFD, and KGM supplementation, particularly at 1% inclusion, significantly improved growth performance and feed efficiency in fish fed both BFD and HFD. In addition, Pompano fed HFD showed higher whole-body lipid and lower protein contents than fish fed BFD. Irrespective of dietary lipid level, whole body, dorsal and ventral muscle were reduced by dietary KGM supplementation up to 1% while the opposite was found for whole body protein content. Dietary KGM also significantly reduced the HSI, liver lipid contents, as well as lipid droplets in the liver. Supplementation of KGM to both BFD and HFD significantly reduced serum total cholesterol, triglyceride, low-density lipoprotein cholesterol and free fatty acids levels. Furthermore, irrespective of dietary lipid level, increasing dietary KGM supplementation enhanced serum IgM level, lysozyme and alkaline phosphatase activities, and reduced serum alanine aminotransferase and aspartate aminotransferase activities. Dietary KGM also increased liver total antioxidant capacity, superoxide dismutase and catalase activities, and decreased malondialdehyde concentration in fish fed both HFD and BFD. Dietary KGM significantly increased serum glutathione peroxidase, superoxide dismutase and catalase activities and reduced malondialdehyde concentrations in fish fed BFD, and enhanced serum total antioxidant capacity and glutathione peroxidase activity in fish fed HFD. Supplementation of KGM increased the expression of genes associated with lipid catabolism (pparα, cpt1, hsl) and lipid transport (fabp1, apoB100 and cd36), and decreased the expression of lipid anabolism related genes (srebp1, pparγ, fas and acc). Moreover, the expression of pro-inflammatory cytokines including tumor necrosis factor α and interleukin-8 were down-regulated, while expression of the anti-inflammatory cytokine transforming growth factor β1 was increased by KGM supplementation. In conclusion, supplementation of KGM at 1% of diet improved growth, feed efficiency, antioxidant capacity, hepatic lipid metabolism and inflammatory responses in juvenile pompano, and can mitigate excessive deposition of lipid in tissues of farmed fish.This research was supported by the China Agricultural Research System (CARS-47), Guangdong Agriculture Research System (2021KJ150), Natural Science Foundation of Guangdong Province (2020A1515011252), Innovation and Strong School Projects in Guangdong Province (2020ZDZXlO14) and Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0606).ElsevierChina Agricultural UniversityGuangdong Science and Technology DepartmentNatural Science Foundation of Guangdong ProvinceSouthern Marine Science and Engineering Guangdong LaboratoryConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/267098reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.aquaculture.2021.737113Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2670982026-05-22T06:33:51Z
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