In situ rumen degradation of amino acids from different feeds corrected for microbial contamination

[EN]: Variations of the amino acid profile produced by rumen actions were established for fish meal (FM), corn grain (CG), corn gluten feed (GF), dehydrated beet pulp (DBP), and lucerne hay (LH), using the nylon bag technique. The effects of degradation of soluble or insoluble proteins were measured...

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Detalles Bibliográficos
Autores: González, Javier, Centeno, Carmen, Lamrani, Farida, Rodríguez, Carlos Alberto
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/257110
Acceso en línea:http://hdl.handle.net/10261/257110
Access Level:acceso abierto
Palabra clave:Rumen degradation
Microbial contamination
Amino acid profile
Contamination microbienne
Dégradation ruminale
Profil des acides aminés
15N
id ES_c064edbb4825a8e136b6d772fcbeaaa6
oai_identifier_str oai:digital.csic.es:10261/257110
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
Dégradation ruminale in situ des acides aminés de différents aliments, corrigée par la contamination microbienne
title In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
spellingShingle In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
González, Javier
Rumen degradation
Microbial contamination
Amino acid profile
Contamination microbienne
Dégradation ruminale
Profil des acides aminés
15N
title_short In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
title_full In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
title_fullStr In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
title_full_unstemmed In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
title_sort In situ rumen degradation of amino acids from different feeds corrected for microbial contamination
dc.creator.none.fl_str_mv González, Javier
Centeno, Carmen
Lamrani, Farida
Rodríguez, Carlos Alberto
author González, Javier
author_facet González, Javier
Centeno, Carmen
Lamrani, Farida
Rodríguez, Carlos Alberto
author_role author
author2 Centeno, Carmen
Lamrani, Farida
Rodríguez, Carlos Alberto
author2_role author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Rumen degradation
Microbial contamination
Amino acid profile
Contamination microbienne
Dégradation ruminale
Profil des acides aminés
15N
topic Rumen degradation
Microbial contamination
Amino acid profile
Contamination microbienne
Dégradation ruminale
Profil des acides aminés
15N
description [EN]: Variations of the amino acid profile produced by rumen actions were established for fish meal (FM), corn grain (CG), corn gluten feed (GF), dehydrated beet pulp (DBP), and lucerne hay (LH), using the nylon bag technique. The effects of degradation of soluble or insoluble proteins were measured by rumen incubations at 0 h (washout value) and 16 h, respectively. Rumen incubations were performed on four cannulated wethers and the microbial contamination of incubated residues was corrected using a continuous 15N-intraruminal infusion and solid associated bacteria isolates as the reference sample. The results showed that rumen degradation of both soluble and insoluble proteins produced changes in the amino acid profile, which were not uniform among feeds. Differences between feed and insoluble protein amino acid composition were limited for FM and DBP, important for GF and LH, and intermediate for CG. Differences between insoluble and undegraded protein amino acid composition were also limited for FM, GF, DBP, and LH and important in CG. The results showed that the amino acid profile in the undegraded protein and in the whole feed should be close for FM, DBP and LH. On the contrary, in CG, important variations are expected, since both solubility and degradability effects produced changes with the same tendency for most of the essential amino acids. In particular, our results showed a great decrease in the lysine content of the undegraded protein, which increases the lysine deficiency of this feed. This decrease of lysine was also evidenced in GF.
publishDate 2001
dc.date.none.fl_str_mv 2001
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/257110
url http://hdl.handle.net/10261/257110
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv EDP Sciences
publisher.none.fl_str_mv EDP Sciences
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418474032332800
spelling In situ rumen degradation of amino acids from different feeds corrected for microbial contaminationDégradation ruminale in situ des acides aminés de différents aliments, corrigée par la contamination microbienneGonzález, JavierCenteno, CarmenLamrani, FaridaRodríguez, Carlos AlbertoRumen degradationMicrobial contaminationAmino acid profileContamination microbienneDégradation ruminaleProfil des acides aminés15N[EN]: Variations of the amino acid profile produced by rumen actions were established for fish meal (FM), corn grain (CG), corn gluten feed (GF), dehydrated beet pulp (DBP), and lucerne hay (LH), using the nylon bag technique. The effects of degradation of soluble or insoluble proteins were measured by rumen incubations at 0 h (washout value) and 16 h, respectively. Rumen incubations were performed on four cannulated wethers and the microbial contamination of incubated residues was corrected using a continuous 15N-intraruminal infusion and solid associated bacteria isolates as the reference sample. The results showed that rumen degradation of both soluble and insoluble proteins produced changes in the amino acid profile, which were not uniform among feeds. Differences between feed and insoluble protein amino acid composition were limited for FM and DBP, important for GF and LH, and intermediate for CG. Differences between insoluble and undegraded protein amino acid composition were also limited for FM, GF, DBP, and LH and important in CG. The results showed that the amino acid profile in the undegraded protein and in the whole feed should be close for FM, DBP and LH. On the contrary, in CG, important variations are expected, since both solubility and degradability effects produced changes with the same tendency for most of the essential amino acids. In particular, our results showed a great decrease in the lysine content of the undegraded protein, which increases the lysine deficiency of this feed. This decrease of lysine was also evidenced in GF.[FR]: Les variations produites par les actions ruminales sur le profil des acides aminés de la farine de poisson (FP), du grain de maïs (GM), du gluten feed de maïs (GF), de la pulpe de betterave deshydratée (PBD) et du foin de luzerne (FL) ont été déterminées par la méthode des sachets de nylon. Les effets de la dégradation des protéines solubles ou insolubles ont été mesurés en incubant dans le rumen les sachets contenant l’aliment à des temps de séjour de 0 (valeur de lavage) et 16 heures, respectivement. Les incubations ruminales ont été conduites sur quatre béliers porteurs de canules du rumen. Les résultats ont été corrigés en fonction de la contamination microbienne des résidus, moyennant l’infusion en continu de 15N dans le rumen et l’emploi comme référence d’un échantillon de bactéries adhérentes isolées du rumen. Les résultats montrent que la dégradation ruminale tant des protéines solubles que des protéines insolubles donne lieu à des changements du profil des acides aminés. Cependant ces changements ne sont pas uniformes parmi les aliments. La dégradation des protéines solubles a exercée un effet limité pour FP et PBD, alors qu’elle a été importante pour GF et FL et intermédiaire pour GM. Les effets de la dégradation des protéines insolubles ont été limités pour FP, GF, PBD et FL, mais importants pour GM. Les résultats ont montré que le profil des acides aminés des protéines non degradées est similaire à celui de la protéine d’origine pour FP, PBD et FL. Au contraire, pour GM on peut prévoir un important changement, puisque les variations produites par la dégradation des protéines solubles ou insolubles vont dans le même sens pour la plupart des acides aminés essentiels. En particulier, nos résultats montrent un fort appauvrisement de la teneur en lysine dans les protéines non degradées, ce qui accroît sa déficience dans l’aliment. Cette réduction de la teneur en lysine est démontrée aussi pour GF.This work has been supported by the CICYT funded Projects AGF 93-0549-CO2-01 and AGF 98-0842.Peer reviewedEDP SciencesComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212001info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/257110reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2571102026-05-22T06:33:51Z
score 15,812429

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