Gibberellin Regulation of Fruit-Set and Growth in Tomato

The role of gibberellins (GAs) in tomato (Solanum lycopersicum L.) fruit development was 3 investigated. Two different inhibitors of GA biosynthesis (LAB 198999 and paclobutrazol) 4 decreased fruit growth and fruit-set, an effect reversed by GA3 application. LAB 198999 5 reduced GA1 and GA8 content,...

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Autores: Serrani, Juan Carlos, Sanjuán, Rafael, Ruiz-Rivero, Omar, Fos, Mariano, García-Martínez, José-Luis
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/12353
Acceso en línea:http://hdl.handle.net/10261/12353
Access Level:acceso abierto
Palabra clave:Fruit-set
Gibberellins
Tomato
Micro-Tom
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spelling Gibberellin Regulation of Fruit-Set and Growth in TomatoSerrani, Juan CarlosSanjuán, RafaelRuiz-Rivero, OmarFos, MarianoGarcía-Martínez, José-LuisFruit-setGibberellinsTomatoMicro-TomThe role of gibberellins (GAs) in tomato (Solanum lycopersicum L.) fruit development was 3 investigated. Two different inhibitors of GA biosynthesis (LAB 198999 and paclobutrazol) 4 decreased fruit growth and fruit-set, an effect reversed by GA3 application. LAB 198999 5 reduced GA1 and GA8 content, but increased that of their precursors GA53, GA44, GA19 and 6 GA20 in pollinated fruits. This supports the hypothesis that GA1 is the active GA for tomato fruit 7 growth. Unpollinated ovaries developed parthenocarpically in response to GA3 > GA1 = GA4 > 8 GA20, but not to GA19, suggesting that GA 20-oxidase activity was limiting in unpollinated 9 ovaries. This was confirmed by analyzing the effect of pollination on transcript levels of SlCPS, 10 SlGA20ox1, -2 and -3, and SlGA3ox1 and -2, encoding enzymes of GA biosynthesis. Pollination 11 increased transcript content of SlGA20ox1, -2, and -3, and SlCPS, but not of SlGA3ox1 and -2. 12 To investigate whether pollination also altered GA inactivation, full length cDNA clones of 13 genes encoding enzymes catalyzing GA 2-oxidases (SlGA2ox1, -2, -3-, -4 and -5) were isolated 14 and characterized. Transcript levels of these genes did not decrease early after pollination (5-d15 old fruits), but transcript content reduction of all of them, mainly of SlGA2ox2, was found later 16 (from 10 d after anthesis). We conclude that pollination mediates fruit-set by activating GA 17 biosynthesis mainly through up regulation of GA20ox. Finally, the phylogenetic reconstruction 18 of the GA2ox familiy clearly showed the existence of three gene subfamilies, and the 19 phylogenetic position of SlGA2ox1, -2, -3, -4 and -5 was established.This work was supported by grants from the Ministerio de Ciencia y Tecnologia of Spain 3 (BIO2003-00151 and BIO2006-13437)Peer reviewedAmerican Society of Plant Physiologists200920092007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65011778808 bytesapplication/pdfhttp://hdl.handle.net/10261/12353reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1104/pp.107.098335info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/123532026-05-22T06:33:51Z
dc.title.none.fl_str_mv Gibberellin Regulation of Fruit-Set and Growth in Tomato
title Gibberellin Regulation of Fruit-Set and Growth in Tomato
spellingShingle Gibberellin Regulation of Fruit-Set and Growth in Tomato
Serrani, Juan Carlos
Fruit-set
Gibberellins
Tomato
Micro-Tom
title_short Gibberellin Regulation of Fruit-Set and Growth in Tomato
title_full Gibberellin Regulation of Fruit-Set and Growth in Tomato
title_fullStr Gibberellin Regulation of Fruit-Set and Growth in Tomato
title_full_unstemmed Gibberellin Regulation of Fruit-Set and Growth in Tomato
title_sort Gibberellin Regulation of Fruit-Set and Growth in Tomato
dc.creator.none.fl_str_mv Serrani, Juan Carlos
Sanjuán, Rafael
Ruiz-Rivero, Omar
Fos, Mariano
García-Martínez, José-Luis
author Serrani, Juan Carlos
author_facet Serrani, Juan Carlos
Sanjuán, Rafael
Ruiz-Rivero, Omar
Fos, Mariano
García-Martínez, José-Luis
author_role author
author2 Sanjuán, Rafael
Ruiz-Rivero, Omar
Fos, Mariano
García-Martínez, José-Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fruit-set
Gibberellins
Tomato
Micro-Tom
topic Fruit-set
Gibberellins
Tomato
Micro-Tom
description The role of gibberellins (GAs) in tomato (Solanum lycopersicum L.) fruit development was 3 investigated. Two different inhibitors of GA biosynthesis (LAB 198999 and paclobutrazol) 4 decreased fruit growth and fruit-set, an effect reversed by GA3 application. LAB 198999 5 reduced GA1 and GA8 content, but increased that of their precursors GA53, GA44, GA19 and 6 GA20 in pollinated fruits. This supports the hypothesis that GA1 is the active GA for tomato fruit 7 growth. Unpollinated ovaries developed parthenocarpically in response to GA3 > GA1 = GA4 > 8 GA20, but not to GA19, suggesting that GA 20-oxidase activity was limiting in unpollinated 9 ovaries. This was confirmed by analyzing the effect of pollination on transcript levels of SlCPS, 10 SlGA20ox1, -2 and -3, and SlGA3ox1 and -2, encoding enzymes of GA biosynthesis. Pollination 11 increased transcript content of SlGA20ox1, -2, and -3, and SlCPS, but not of SlGA3ox1 and -2. 12 To investigate whether pollination also altered GA inactivation, full length cDNA clones of 13 genes encoding enzymes catalyzing GA 2-oxidases (SlGA2ox1, -2, -3-, -4 and -5) were isolated 14 and characterized. Transcript levels of these genes did not decrease early after pollination (5-d15 old fruits), but transcript content reduction of all of them, mainly of SlGA2ox2, was found later 16 (from 10 d after anthesis). We conclude that pollination mediates fruit-set by activating GA 17 biosynthesis mainly through up regulation of GA20ox. Finally, the phylogenetic reconstruction 18 of the GA2ox familiy clearly showed the existence of three gene subfamilies, and the 19 phylogenetic position of SlGA2ox1, -2, -3, -4 and -5 was established.
publishDate 2007
dc.date.none.fl_str_mv 2007
2009
2009
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/12353
url http://hdl.handle.net/10261/12353
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1104/pp.107.098335
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1778808 bytes
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dc.publisher.none.fl_str_mv American Society of Plant Physiologists
publisher.none.fl_str_mv American Society of Plant Physiologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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