The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol

14 pag.- 8 fig.- 5 tab.

Detalles Bibliográficos
Autores: Escapa, Isabel F., Del Cerro, Carlos, García, José Luis, Prieto, María Auxiliadora
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/126478
Acceso en línea:http://hdl.handle.net/10261/126478
Access Level:acceso abierto
Palabra clave:Biotechnology
Gene expression regulation
Glycerol
Pseudomonas putida
Repressor proteins
id ES_bfee7f97074e1b7c88a3a200a9754513
oai_identifier_str oai:digital.csic.es:10261/126478
network_acronym_str ES
network_name_str España
repository_id_str
spelling The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerolEscapa, Isabel F.Del Cerro, CarlosGarcía, José LuisPrieto, María AuxiliadoraBiotechnologyGene expression regulationGlycerolPseudomonas putidaRepressor proteins14 pag.- 8 fig.- 5 tab.Pseudomonas putida KT2440 has evolved a tightly regulated system for metabolizing glycerol implying a prolonged growth lag-phase. We have learnt that this fact can be avoided by the addition of small amounts of some growth precursors. The addition of 1mM octanoic acid as co-feeder completely eliminated the lag-phase, resulting in an improvement, in terms of invested time, of both growth and polyhydroxyalkanoates (PHA) accumulation. To investigate this phenomenon, we have followed co-metabolic approaches combined with mutations of the specific and global regulatory networks that connect glycerol catabolism and PHA synthesis. By using mutant strains in metabolic genes from the PHA and tricarboxylic acid (TCA) cycles, we have demonstrated that the co-feeding effect is independent of PHA accumulation, but driven on active glyoxylate shunt and Entner-Doudoroff (ED) routes. These findings suggested that the effect of octanoate on glycerol metabolism could rely, either on a global activation of the cell energy state, or on the generation of specific metabolites or cofactors needed for the activation of glycerol metabolism. Our results addressed GlpR as the key factor controlling the efficient utilization of glycerol as growth precursor in P.putida KT2440. Accordingly, a glpR knockout mutant of P.putida KT2440 showed an elimination of the lag-phase when cultured on glycerol in the absence of co-feeder. Besides, the production of PHA in this strain was increased near twofold, resulting in a higher final yield in terms of PHA accumulation. The repressor activity of the GlpR protein over the glp genes in the absence of glycerol was finally demonstrated by qRT-PCR. This work contributed to unravel the physiological causes of the long lag-phase produced by glycerol in the model strain P.putida KT2440 that hinders its use as carbon source in biotechnological applications for generating valuable products.This work was supported by 597 grants from the Comisión Interministerial de Ciencia y Tecnología and CSIC598 (BIO2010-21049 and 201120E092), and by the European Union Grant (NMP2-CT-599 2007-026515). Isabel F. Escapa is a recipient of CSIC-I3P predoctoral fellowship.Peer reviewedJohn Wiley & SonsEuropean CommissionComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/126478reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Español#PLACEHOLDER_PARENT_METADATA_VALUE#NMP2-CT-599 2007-026515http://dx.doi.org/doi:10.1111/j.1462-2920.2012.02790.xSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1264782026-05-22T06:33:51Z
dc.title.none.fl_str_mv The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
title The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
spellingShingle The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
Escapa, Isabel F.
Biotechnology
Gene expression regulation
Glycerol
Pseudomonas putida
Repressor proteins
title_short The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
title_full The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
title_fullStr The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
title_full_unstemmed The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
title_sort The role of GlpR repressor in Pseudomonas putida KT2440 growth and PHA production from glycerol
dc.creator.none.fl_str_mv Escapa, Isabel F.
Del Cerro, Carlos
García, José Luis
Prieto, María Auxiliadora
author Escapa, Isabel F.
author_facet Escapa, Isabel F.
Del Cerro, Carlos
García, José Luis
Prieto, María Auxiliadora
author_role author
author2 Del Cerro, Carlos
García, José Luis
Prieto, María Auxiliadora
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biotechnology
Gene expression regulation
Glycerol
Pseudomonas putida
Repressor proteins
topic Biotechnology
Gene expression regulation
Glycerol
Pseudomonas putida
Repressor proteins
description 14 pag.- 8 fig.- 5 tab.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/126478
url http://hdl.handle.net/10261/126478
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
NMP2-CT-599 2007-026515
http://dx.doi.org/doi:10.1111/j.1462-2920.2012.02790.x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418433688371200
score 15,812429

Similares en LA Referencia